1997
DOI: 10.1128/jb.179.10.3298-3303.1997
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Rates of spontaneous mutation in an archaeon from geothermal environments

Abstract: To estimate the efficacy of mechanisms which may prevent or repair thermal damage to DNA in thermophilic archaea, a quantitative assay of forward mutation at extremely high temperature was developed for Sulfolobus acidocaldarius, based on the selection of pyrimidine-requiring mutants resistant to 5-fluoro-orotic acid. Maximum-likelihood analysis of spontaneous mutant distributions in wild-type cultures yielded maximal estimates of (2.8 ؎ 0.7) ؋ 10 ؊7 and (1.5 ؎ 0.6) ؋ 10 ؊7 mutational events per cell per divis… Show more

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Cited by 82 publications
(55 citation statements)
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“…Data from the second f luctuation test were used to confirm the genic mutation rate realized under our conditions. The value (3.37 Ϯ 0.36) ϫ 10 Ϫ7 , agrees well with an average value of (3.29 Ϯ 1.35) ϫ 10 Ϫ7 determined previously for larger cultures under similar but not identical growth conditions (5). The 79 intragenic pyrE mutations from the first two tests were pooled and designated set I.…”
Section: Resultssupporting
confidence: 70%
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“…Data from the second f luctuation test were used to confirm the genic mutation rate realized under our conditions. The value (3.37 Ϯ 0.36) ϫ 10 Ϫ7 , agrees well with an average value of (3.29 Ϯ 1.35) ϫ 10 Ϫ7 determined previously for larger cultures under similar but not identical growth conditions (5). The 79 intragenic pyrE mutations from the first two tests were pooled and designated set I.…”
Section: Resultssupporting
confidence: 70%
“…Three corresponding estimates of the mutation rate per cell generation, , were calculated by using the relationship ϭ (ln2)(m͞N av ). There was no apparent bias in the rate estimates as a function of the quartile used, reinforcing direct experimental evidence that possible distortions caused by phenotypic lag or differences in growth rate between mutant and parent strains in uracil-supplemented media are negligible (5).…”
Section: Methodsmentioning
confidence: 72%
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“…This is especially noteworthy given that the primary process that leads to uracil in DNA, cytosine deamination, has an enhanced rate at high temperatures [61]. Yet these organisms do not display a higher mutation rate [62]. Might the presence of this cofactor help fulfill this greater requirement for repair?…”
Section: A Model For Damage Detection Through Dna Charge Transportmentioning
confidence: 99%