Rates of glycogen synthesis and the cellular levels of ATP and FDP during exponential growth and the nitrogen-limited stationary phase of Escherichia coli W4597 (K)

Dietzler, David N.; Leckie, Mary P.; Lais, Cynthia J.

doi:10.1016/0003-9861(73)90321-4

Cited by 49 publications

(29 citation statements)

References 40 publications

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“…ADP-pyrophosphorylase is activated by fructose 1,6-bisphosphate Figure 9. Ef (Preiss et al, 1975), whose intracellular from Takahas level is high under glucose-excess and nitrogen source-limiting conditions (Dietzler et al, 1973). On the other hand, the ADP-glucose pyrophosphorylase is inhibited by ADP, AMP, and/or Pi (Preiss and Romeo, 1989), whose levels are expected to be high under glucose-limiting conditions.…”

Section: (Viic) Phmentioning

confidence: 99%

Glucose and Lactate Metabolism By Actinomyces Naeslundii

Takahashi

Yamada

1999

Critical Reviews in Oral Biology & Medicine

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Actinomyces are among the predominant bacteria in the oral microflora. This review discusses the glucose and lactate metabolism of Actinomyces naeslundii and its ecological significance in dental plaque. This bacterium has the EmbdenMeyerhof-Parnas (EMP) pathway as the main route to degrade glucose. The EMP pathway-derived metabolic intermediates, phosphoenolpyruvate (PEP) and pyruvate, are further converted into different end-products, depending on the environment. Under anaerobic conditions in the absence of bicarbonate, the pyruvate is converted into lactate by a lactate dehydrogenase. In the presence of bicarbonate, the PEP is combined with bicarbonate and then converted into succinate through the succinate pathway, while the pyruvate is converted into formate and acetate through the pyruvate formate-lyase pathway. Under aerobic conditions, the pyruvate liberates acetate and CO2 through a pathway initiated by a pyruvate dehydrogenase. A. naeslundii strains also degrade lactate, aerobically, to acetate and CO2 through the conversion of lactate into pyruvate by a NADindependent lactate dehydrogenase. These strains also synthesize glycogen from a glycolytic intermediate, glucose 6-phosphate. Besides atmospheric conditions and bicarbonate, the intracellular reduction-oxidation potential, carbohydrate concentration, and environmental pH also modulate the metabolism of A. naeslundii. Some of the phosphorylating enzymes involved in A. naeslundii metabolism-e.g., GTP/polyphosphate (PPn)-dependent glucokinase, pyrophosphate (PPi)-dependent phosphofructokinase, UDP-glucose pyrophosphorylase, and GDP/IDP-dependent PEP carboxykinase-are unique to A. naeslundii and have not been found in other oral bacteria. The utilization of PPn and PPi as phosphoryl donors, together with glycogen synthesis and lactate utilization, could contribute to the efficient energy metabolism found in A. naeslundii. Through this flexible and efficient metabolic capacity, A. naeslundii can adapt to fluctuating environments and compete with other bacteria in dental plaque. Further, this bacterium may modify the dental plaque environment and promote the microbial population shifts in dental plaque.

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Section: (Viic) Phmentioning

confidence: 99%

Glucose and Lactate Metabolism By Actinomyces Naeslundii

Takahashi

Yamada

1999

Critical Reviews in Oral Biology & Medicine

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“…The physiological energy charge range in various E. coli strains is found to be 0.74-0.9 (69,73). The physiological concentrations of fructose-P2 ranges between 0.71 and 3 m M (68,69). Under these conditions the C L l l 3 6 enzyme shows maximal activity and is not sensitive either to change in energy charge or to fluctuations of fructose-P2.…”

Section: Table Xi1mentioning

confidence: 99%

“…Dietzler et al (68,69) have shown that when the growth of E. coli W4597(K) ceased because of NH,+ depletion, the rate of glycogen accumulation increased about 3.3-to 4.2-fold. Concomitant with this, a decrease of fructose-P2 of about 76% (from 3.1 to 0.71 mM) and an increase in the energy charge from 0.74 in exponential phase to 0.87 in stationary phase occurred (68,69).…”

Section: B Kelationships Of Fructose-pz Concentrations and Energy Chmentioning

confidence: 99%

“…Concomitant with this, a decrease of fructose-P2 of about 76% (from 3.1 to 0.71 mM) and an increase in the energy charge from 0.74 in exponential phase to 0.87 in stationary phase occurred (68,69). The total concentration of the adenylate pool (ATP + AMP + ADP) in exponential and stationary phase was 3 mM.…”

Section: B Kelationships Of Fructose-pz Concentrations and Energy Chmentioning

confidence: 99%

“…As mcntioned previously, the total adenine nucleotide concentration is about 3 mM (69-71), the fructose-pz concentration ranges from 0.71 to 3.2 mM (68-71), the mass action ratio of the adenylate kinase reaction ranges from 0.37 to 0.69, and energy charge values lie between 0.74 and 0.9 (68)(69)(70)(71)(72)(73)98) in various E. coli strains grown in nitrogen-limiting minimal media, with glucose as the carbon source.…”

Section: B Kelationships Of Fructose-pz Concentrations and Energy Chmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of Adenosine Diphosphate Glucose Pyrophosphorylase

Preiss

1978

Advances in Enzymology - And Related Areas of Molecular Biology

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Fructose-1,6-bisphosphate, a regulator of metabolism

Kirtley

McKay

1977

Mol Cell Biochem

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Fructose-1,6-bisphosphate affects the rate of a large variety of enzyme reactions. In some instances its role as a physiologic effector is well documented. In many cases the effects of fructose bishosphate on particular enzymes have been demonstrated in vitro but the link to physiologic conditions has not yet been established. It is the purpose of this paper to summarize the scattered findings in fructose bisphosphate as an effector of enzyme reactions and to draw some conclusions about the role of the compound in metabolic regulation.

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Rates of glycogen synthesis and the cellular levels of ATP and FDP during exponential growth and the nitrogen-limited stationary phase of Escherichia coli W4597 (K)

Cited by 49 publications

References 40 publications

Glucose and Lactate Metabolism By Actinomyces Naeslundii

Glucose and Lactate Metabolism By Actinomyces Naeslundii

Regulation of Adenosine Diphosphate Glucose Pyrophosphorylase

Fructose-1,6-bisphosphate, a regulator of metabolism

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