Rates of chilling to 0°C: implications for the survival of microorganisms and relationship with membrane fluidity modifications

Cao-Hoang, Lan; Dumont, Frédéric; Marechal, Pierre-André; Lê-Thanh, Mai; Gervais, Patrick

doi:10.1007/s00253-007-1279-z

Cited by 39 publications

(21 citation statements)

References 32 publications

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“…This observation emphasizes the fact that yeast cells are adapted to cooler temperatures than the latter two cell types (Inouye and Phadtare, 2004), and could also be related to the presence of trehalose and high level of sterols in the membranes of yeast cells. These processes are indeed believed to underlie the resistance of cell membranes to cold stress (Cao-Hoang et al, 2008). On the other hand, leukemia cells were the most sensitive to cold stress.…”

Section: Time-dependent Loss Of Cell Viabilitymentioning

confidence: 99%

Cell inactivation and membrane damage after long‐term treatments at sub‐zero temperature in the supercooled and frozen states

Moussa¹,

Dumont²,

Perrier-Cornet³

et al. 2008

Biotech & Bioengineering

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The survival of cells subjected to cooling at sub-zero temperature is of paramount concern in cryobiology. The susceptibility of cells to cryopreservation processes, especially freeze-thawing, stimulated considerable interest in better understanding the mechanisms leading to cell injury and inactivation. In this study, we assessed the viability of cells subjected to cold stress, through long-term supercooling experiments, versus freeze-thawing stress. The viability of Escherichia coli, Saccharomyces cerevisiae, and leukemia cells were assessed over time. Supercooled conditions were maintained for 71 days at -10 degrees C, and for 4 h at -15 degrees C, and -20 degrees C, without additives or emulsification. Results showed that cells could be inactivated by the only action of sub-zero temperature, that is, without any water crystallization. The loss of cell viability upon exposure to sub-zero temperatures is suggested to be caused by exposure to cold shock which induced membrane damage. During holding time in the supercooled state, elevated membrane permeability results in uncontrolled mass transfer to and from the cell maintained at cold conditions and thus leads to a loss of viability. With water crystallization, cells shrink suddenly and thus are exposed to cold osmotic shock, which is suggested to induce abrupt loss of cell viability. During holding time in the frozen state, cells remain suspended in the residual unfrozen fraction of the liquid and are exposed to cold stress that would cause membrane damage and loss of viability over time. However, the severity of such a stress seems to be moderated by the cell type and the increased solute concentration in the unfrozen fraction of the cell suspension.

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Section: Time-dependent Loss Of Cell Viabilitymentioning

confidence: 99%

Cell inactivation and membrane damage after long‐term treatments at sub‐zero temperature in the supercooled and frozen states

Moussa¹,

Dumont²,

Perrier-Cornet³

et al. 2008

Biotech & Bioengineering

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show abstract

“…For this purpose, 3 ml of YPD/30ºC-grown cells (OD 600 0.5) were harvested, treated with DPH solution and analyzed with a spectrofluorometer (Fluorolog-3, Horiva Jobin Yvon, Edison, NJ), as previously described [11].…”

Section: Fluorescence Anisotropy Measurementsmentioning

confidence: 99%

Characterization of the S. cerevisiae inp51 mutant links phosphatidylinositol 4,5-bisphosphate levels with lipid content, membrane fluidity and cold growth

Córcoles-Sáez

Hernández

Martínez-Rivas

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…Growth of bacteria at temperatures below their optimum and particularly below the socalled "normal range" (10), requires a number of phenotypic changes. These include alterations in cell membranes by increasing unsaturated fatty acid content, changes in the transcriptional and translational machinery, and the production of cold shock and cold acclimation proteins (11,12). Bacteria respond to hyperosmotic stress (or low water activity, a w ) by activating osmoregulatory systems that cause accumulation of charged solutes (e.g.…”

mentioning

confidence: 99%

Integrated Transcriptomic and Proteomic Analysis of the Physiological Response of Escherichia coli O157:H7 Sakai to Steady-state Conditions of Cold and Water Activity Stress

Kocharunchitt

King

Gobius

et al. 2012

Molecular & Cellular Proteomics

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An integrated transcriptomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass chilling in cold air. The response of E. coli during exponential growth at 25°C a w 0.985, 14°C a w 0.985, 25°C a w 0.967, and 14°C a w 0.967 was compared with that of a reference culture (35°C a w 0.993). Gene and protein expression profiles of E. coli were more strongly affected by low water activity (a w 0.967) than by low temperature (14°C). Predefined group enrichment analysis revealed that a universal response of E. coli to all test conditions included activation of the master stress response regulator RpoS and the Rcs phosphorelay system involved in the biosynthesis of the exopolysaccharide colanic acid, as well as down-regulation of elements involved in chemotaxis and motility. However, colanic acid-deficient mutants were shown to achieve comparable growth rates to their wild-type parents under all conditions, indicating that colanic acid is not required for growth. In contrast to the transcriptomic data, the proteomic data revealed that several processes involved in protein synthesis were down-regulated in overall expression at 14°C a w 0.985, 25°C a w 0.967, and 14°C a w 0.967. This result suggests that during growth under these conditions, E. coli, although able to transcribe the required mRNA, may lack the cellular resources required for translation. Elucidating the global adaptive response of E. coli O157:H7 during exposure to chilling and water activity stress has provided a baseline of knowledge of the physiology of this pathogen.

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Rates of chilling to 0°C: implications for the survival of microorganisms and relationship with membrane fluidity modifications

Cited by 39 publications

References 32 publications

Cell inactivation and membrane damage after long‐term treatments at sub‐zero temperature in the supercooled and frozen states

Cell inactivation and membrane damage after long‐term treatments at sub‐zero temperature in the supercooled and frozen states

Characterization of the S. cerevisiae inp51 mutant links phosphatidylinositol 4,5-bisphosphate levels with lipid content, membrane fluidity and cold growth

Integrated Transcriptomic and Proteomic Analysis of the Physiological Response of Escherichia coli O157:H7 Sakai to Steady-state Conditions of Cold and Water Activity Stress

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