Rate of Ingestion and Proteolytic Activity in Digestive System of Juvenile White Shrimp,Penaeus vannamei, During Continual Feeding

Abstract

Juvenile white shrimp, Penaeus vannamei, were studied using tray feeders in relation to food ingestion and enzymatic activity, with data taken every two hours for five days. The feed ingestion was calculated by subtracting the uningested and the leached material from the food provided. Significant differences (P < 0.05) in the ingestion rate were found during the experiment. The greatest ingestion occurred between 2000 and 2200 hours. The largest feed ingestion coincided with the nocturnal activity of shrimp. Time-series analysis showed a cycle of increased ingestion before midnight and lower ingestion during daylight. Total protease, trypsin, and chymotrypsin activity were measured during the last 24 hours of the study and included both fed and starved organisms. The enzymatic pattern was similar for both groups. There was a decrease of the protease and trypsin activities before and after 1800 hours, just before the ingestion increased. The study of digestive enzymes provided evidence of a close association between behavior and regulation of the digestion system physiology. The results

Effect of feeding frequency on growth and enzymatic activity ofLitopenaeus vannameiduring nursery phase in biofloc system

“…Hernandez‐Cortes et al. () investigated the enzymatic profile of juvenile L. vannamei under continuous food intake and, in the period of 24 hr, found that there was an oscillation of the proteolytic (1–2 U/mg), trypsin (2–6 U/mg) and chymotrypsin activities (15–25 U/mg). These values, except for chymotrypsin, were lower than those found in the present study, but the oscillatory pattern of enzymatic activity was also observed throughout the culture period.…”

Influence of dietary protein levels on growth, digestibility, digestive enzyme activity and stress tolerance in white-leg shrimp, Litopenaeus vannamei (Boone, 1931), reared in high-density tank trials

“…Apparent digestibility coefficient (ADC) assessment of feedstuffs is an essential prerequisite in determining the nutrient requirements precisely and in the development of nutritionally adequate diets at the least cost (Irvin & Tabrett 2005; Irvin & Williams 2007; Luo, Tan, Chen, Wang & Zhou 2008). Studies in shrimp have indicated that the dietary protein (DP) level can alter the level of enzyme activity (Hernandez‐Cortes, Quadros, Navarrete, Portillo, Colado & Garcia‐Carreno 1999; Guzman, Gaxiola, Rosas & Torre‐Blanco 2001; Gamboa‐Delgado, Molina‐Poveda & Cahu 2003; Sainz & Cordova‐Murueta 2009). Lee, Smith and Lawrence (1984) observed an increase in the activity of enzymes from the digestive system of L. vannamei , whereas in northern white shrimp Litopenaeus setiferus (Linnaeus, 1767), enzyme activities were reduced as a consequence of increases in the protein concentration in the feed.…”

Effect of different diets on digestive enzyme activities, in vitro digestibility, and midgut gland structure in juvenile crayfish, Cherax quadricarinatus

“…Serine proteinases, such as trypsin and chymotrypsin, are proposed to be the most important crustacean digestive enzymes, believed to be responsible for 40–60% of total protein digestion in penaeids (Hernández‐Cortés et al . ). Teschke and Saborowski () demonstrated that cysteine proteinases were the predominant enzymes in the midgut gland of Crangon crangon and C. allmani , but their activity was the lowest in Euphausia superba .…”