Rate of basement membrane biosynthesis as an index to angiogenesis

Maragoudakis, Michael E.; Panoutsacopoulou, M; Sarmonika, M

doi:10.1016/0040-8166(88)90055-9

Cited by 85 publications

(60 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The concept of an angiogenic profile encompassing several angiogenic pathways might be more useful and measures of capillary maturation should be considered as a potential part. This might become more important with the advent of anti-angiogenic agents directed against particular processes involved in vascular remodelling, such as EC migration, cell-matrix interactions and secretion of basement membrane (Ingber and Folkman, 1988;Maragoudakis et al, 1988;Nicosia and Bonano, 1991;Sakamoto et al, 1991;Kibbey et al, 1992;Grant et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Relationship of vascular maturation in breast cancer blood vessels to vascular density and metastasis, assessed by expression of a novel basement membrane component, LH39

et al. 2000

View full text Add to dashboard Cite

Angiogenesis, the formation of new vessels, has been demonstrated to be an indicator of prognosis in breast cancer patients. The extent of differentiation of the tumour vessels may affect access of peripheral white cells and egress or invasion of tumour cells. This has not been assessed in relation to tumour microvessel density or other variables and may be a marker of vascular remodelling. LH39 is a monoclonal antibody recognizing an epitope located at the lamina lucida of mature small veins and capillaries but not in newly formed vessels. To study vascular differentiation in breast tumours, we examined the vascular maturation index (VMI) in 12 normal and 50 breast carcinomas and this was correlated with different clinicopathological variables including angiogenesis. Mature vessels were defined by staining with antibodies to both LH39 and to CD31, using double immunohistochemistry, whereas immature vessels stained only for CD31. VMI was defined as the % fraction of mature vessels (LH39-positive) / total number of vessels (CD31-positive). The VMI was significantly higher in normal (54–68.5%; median 66.5%) than in tumours (0–47%; median 8.8%) ( P = 0.0005). There was a significant inverse correlation between the tumour VMI and nodal status (Fisher's exact test, P = 0.01) and between high VMI and low thymidine phosphorylase (TP) expression (Mann–Whitney U -test, P = 0.01). No significant association between VMI and tumour size, oestrogen receptor, epidermal growth factor receptor, grade, angiogenesis, patient age, or E-selectin was seen. There was a significant reduction in relapse-free survival ( P = 0.01) with high angiogenesis. These findings show that the VMI gives new information on the mechanism of tumour angiogenesis independently from microvessel quantitation, there is a wide variation in the differentiation of tumour vasculature but the degree of capillary differentiation is not associated with quantitative angiogenesis. The VMI identifies a subset of patients who have a high chance of regional node involvement. © 2000 Cancer Research Campaign

show abstract

Section: Discussionmentioning

confidence: 99%

Relationship of vascular maturation in breast cancer blood vessels to vascular density and metastasis, assessed by expression of a novel basement membrane component, LH39

et al. 2000

View full text Add to dashboard Cite

show abstract

“…British Journal of Pharmacology (1995Pharmacology ( ) 116, 1829Pharmacology ( 1834 Biochemical evaluation of angiogenesis Collagenous proteins represent 80% of the total basement membrane proteins formed by the CAM during the chick embryo development (Maragoudakis et al, 1988). The extent of their biosynthesis has been shown to correlate well with new vessel formation.…”

Section: Methodsmentioning

confidence: 99%

“…The in vivo CAM angiogenesis model, initially described by Folkman (1985) and modified as previously reported (Maragoudakis et al, 1988) was used. Briefly, fresh fertilized eggs were incubated for 4 days at 37°C when a window was opened on the egg shell exposing the CAM.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of angiogenesis, tumour growth and metastasis by the NO‐releasing vasodilators, isosorbide mononitrate and dinitrate

Pipili-Synetos¹,

Παπαγεωργίου²,

Sakkoula³

et al. 1995

British J Pharmacology

137

View full text Add to dashboard Cite

The effect of the nitric oxide (NO)‐producing nitrovasodilators isosorbide mononitrate (ISMN) and isosorbide dinitrate (ISDN) were assessed on (a) the in vivo model of angiogenesis of the chick chorioallantoic membrane (CAM) and (b) on the growth and metastatic properties of the Lewis Lung carcinoma (LLC) in mice Isosorbide 5‐mononitrate (ISMN) and isosorbide dinitrate (ISDN), inhibited angiogenesis in the CAM dose‐dependently. ISMN was more potent in inhibiting this process. Both compounds were capable of completely reversing the angiogenic effect of α‐thrombin. These effects of ISMN and ISDN on angiogenesis were comparable to those previously observed with sodium nitroprusside which generates NO non‐enzymatically Mice, implanted intramuscularly with LLC, received daily i.p. injections of ISMN for 14 days resulting in a significant decrease in the size of the primary tumour and a reduction in the number and size of metastatic foci in the lungs. ISDN had a similar but less pronounced effect than that observed with ISMN Addition of ISMN or ISDN to cultures of bovine, rabbit and human endothelial cells and to cultures of LLC cells had no effect on their growth characteristics These results indicate that ISMN and ISDN inhibit angiogenesis and tumour growth and metastasis in an animal tumour model. The possibility should therefore be considered that these nitrovasodilators which are widely used therapeutically and have well characterized pharmacological profiles, may also possess antitumour properties in the clinic.

show abstract

“…The CAM assay was performed as described by Maragoudakis et al [32] with some modifications. Briefly, fertilised eggs were incubated for 3 days at 37°C.…”

Section: Cam Assaymentioning

confidence: 99%

Soluble N-cadherin fragment promotes angiogenesis

Derycke

Morbidelli

Ziche

et al. 2006

Clin Exp Metastasis

View full text Add to dashboard Cite

Endothelial cells express two dependent intercellular adhesion molecules: vascular endothelial (VE)-cadherin, specific for endothelial cells, and Ncadherin, also present in neuronal, lens, skeletal and heart muscle cells, osteoblasts, pericytes and fibroblasts. While there exists a vast amount of evidence that VE-cadherin promotes angiogenesis, the role of N-cadherin still remains to be elucidated. We found that a soluble 90-kDa fragment N-cadherin promotes angiogenesis in the rabbit cornea assay and in the chorioallantoic assay when cleaved enzymatically from the extracellular domain of N-cadherin. Soluble N-cadherin stimulates migration of endothelial cells in the wound healing assay and stimulates phosphorylation of extracellular regulated kinase. In vitro experiments with PD173074 and knock-down of N-cadherin and fibroblast growth factor (FGF)-receptor, showed that the pro-angiogenic effect of soluble N-cadherin is N-cadherin-and FGF-receptor-dependent. Our results suggest that soluble N-cadherin stimulates migration of endothelial cells through the FGF-receptor.

show abstract

Rate of basement membrane biosynthesis as an index to angiogenesis

Cited by 85 publications

References 20 publications

Relationship of vascular maturation in breast cancer blood vessels to vascular density and metastasis, assessed by expression of a novel basement membrane component, LH39

Relationship of vascular maturation in breast cancer blood vessels to vascular density and metastasis, assessed by expression of a novel basement membrane component, LH39

Inhibition of angiogenesis, tumour growth and metastasis by the NO‐releasing vasodilators, isosorbide mononitrate and dinitrate

Soluble N-cadherin fragment promotes angiogenesis

Contact Info

Product

Resources

About