Rat small intestine expresses the reelin–Disabled‐1 signalling pathway

García‐Miranda, Pablo; Peral, María J.; Ilundáin, A.

doi:10.1113/expphysiol.2009.050682

Cited by 29 publications

(59 citation statements)

References 34 publications

(53 reference statements)

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“…6 We previously proposed that in the rat small intestine the reelin released by the myofibroblasts might regulate the crypt-villus unit homeostasis 9 and the current data support this point of view. The results also show that the epithelial cell renewal processes vary with age, as previously reported for control mice, 15 and that reelin does not modify the pattern of this age-related changes.…”

Section: Discussionsupporting

confidence: 76%

“…9 Briefly, the small intestine was rapidly removed and washed with an ice-cold saline solution. One centimeter intestinal segments were incubated at room temperature in the phosphate-buffered saline (PBS) buffer containing 1 mM dithiothreitol for 15 min, followed by a 30-min incubation period at 37°C in a calciumand magnesium-free PBS buffer containing 1 mM EDTA and 2 mM glucose.…”

Section: Isolation Of Enterocytes and Myofibroblatsmentioning

confidence: 99%

“…Immunostaining assays were performed on intact small intestine (either 10-mm cryosections or 5-mm paraffin sections) and on isolated intestinal cells, as previously. 9 The slides containing either the intestinal sections or the isolated cells were incubated with the indicated primary antibody at 4°C, overnight. Antibody binding was visualized with biotinylated anti IgG antibodies, at dilution 1:100, followed by immunoperoxidase staining.…”

Section: Immunostaining Analysismentioning

confidence: 99%

“…9 In brain, the reelin secreted by the CajalRetzius cells is critical for the positioning of migrating neurons during the development of the central nervous system. 10 Because differentiation of the intestinal epithelial cells requires their migration along the crypt-villus axis, we reasoned that the reelin released by the myofibroblasts to the ECM might regulate epithelial dynamics.…”

Section: Introductionmentioning

confidence: 99%

“…10 Because differentiation of the intestinal epithelial cells requires their migration along the crypt-villus axis, we reasoned that the reelin released by the myofibroblasts to the ECM might regulate epithelial dynamics. 9 The present work explores in vivo whether reelin is involved in the crypt-villus unit homeostasis. For that, we have examined the consequences of reelin gene disruption on cell proliferation, migration, differentiation, and apoptosis in the epithelium of mice small intestine.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Reelin Is Involved in the Crypt-Villus Unit Homeostasis

García‐Miranda

Vázquez‐Carretero

Sesma

et al. 2013

Tissue Engineering Part A

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Intestinal myofibroblasts secrete substances that control organogenesis and wound repair of the intestine. The myofibroblasts of the rat small intestine express reelin and the present work explores whether reelin regulates crypt-villus unit homeostasis using normal mice and mice with the reelin gene disrupted (reeler). The results reveal that mouse small intestine expresses reelin, its receptors apolipoprotein E receptor 2 (ApoER2) and very low-density lipoprotein receptor (VldlR) and the reelin effector protein Disabled-1 (Dab1) and that reelin expression is restricted to myofibroblasts. The absence of reelin significantly reduces epithelial cell proliferation, migration, and apoptosis and the number of Paneth cells. These effects are observed during the suckling, weaning, and adult periods. The number of Goblet cells is increased in the 2-month-old reeler mice. The absence of reelin also expands the extracellular space of the adherens junctions and desmosomes without significantly affecting either the tight-junction structure or the epithelial paracellular permeability. In conclusion, this is the first in vivo work showing that the absence of reelin alters intestinal epithelium homeostasis.

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Section: Discussionsupporting

confidence: 76%

Section: Isolation Of Enterocytes and Myofibroblatsmentioning

confidence: 99%

Section: Immunostaining Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reelin Is Involved in the Crypt-Villus Unit Homeostasis

García‐Miranda

Vázquez‐Carretero

Sesma

et al. 2013

Tissue Engineering Part A

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Nonneuronal roles for the reelin signaling pathway

Khialeeva

Carpenter

2016

Developmental Dynamics

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The reelin signaling pathway has been established as an important regulator of cell migration during development of the central nervous system, and disruptions in reelin signaling alter the positioning of many types of neurons. Reelin is a large extracellular matrix glycoprotein and governs cell migration through activation of multiple intracellular signaling events by means of the receptors ApoE receptor 2 (ApoER2) and very low density lipoprotein receptor (VLDLR), and the intracellular adaptor protein Disabled-1 (Dab1). Earlier studies reported expression of reelin in nonneuronal tissues, but the functions of this signaling pathway outside of the nervous system have not been studied until recently. A large body of evidence now suggests that reelin functions during development and disease of multiple nonneuronal tissues. This review addresses recent advances in the field of nonneuronal reelin signaling. Developmental Dynamics 246:217-226,

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Regulation of Dab2 expression in intestinal and renal epithelia by development

Vázquez‐Carretero

García‐Miranda

Calonge

et al. 2011

J of Cellular Biochemistry

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Disabled-2 (Dab2) is an intracellular adaptor protein proposed to function in endocytosis. Here, we investigate the intestinal and renal Dab2 expression versus maturation. Dab2 mRNA levels measured by RT-PCR are greater in the small than in the large intestine. Immunological studies localize Dab2 to the terminal web domain of the enterocytes and reveal the presence of a 96-kDa Dab2 isoform in the apical membrane of the jejunum, ileum, and renal cortex of the suckling and adult rat. A 69-kDa Dab2 isoform is only observed in the apical membranes of the suckling ileum. During the suckling period, the Dab2 mRNA levels measured in the enterocytes and crypts and those of the 96-kDa Dab2 isoform are greater in the ileum than in the jejunum. No segmental differences are observed in the adult intestine. In the intestine, the levels of Dab2 mRNA and those of the 96-kDa Dab2 isoform decrease to adult values at weaning, whereas in the kidney they increase with development. Weaning the pups on a commercial milk diet slows the periweaning decline in the levels of Dab2 mRNA in the crypts and of those of the 96-kDa isoform. This is the first report showing that the 96-kDa Dab2 isoform is expressed at the apical domain of rat small intestine, that ontogeny regulates Dab2 gene expression in intestine and kidney and that retarding weaning affects intestinal Dab2 gene expression.

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Rat small intestine expresses the reelin–Disabled‐1 signalling pathway

Cited by 29 publications

References 34 publications

Reelin Is Involved in the Crypt-Villus Unit Homeostasis

Reelin Is Involved in the Crypt-Villus Unit Homeostasis

Nonneuronal roles for the reelin signaling pathway

Regulation of Dab2 expression in intestinal and renal epithelia by development

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