Rat model of mild stenosis of the common bile duct

Rodríguez-Garay, Emilio A.; Agüero, Rut M.; Pisani, G; Trbojevich, Raúl; A, Farroni; Viglianco, Ricardo A.

doi:10.1007/bf02576832

Cited by 12 publications

(10 citation statements)

References 21 publications

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“…The present study of bile secretion in rats with MSBD was stimulated by the observation reported previously that bile flow and bile acid secretion remained normal at 7-10 days after the surgical procedure, despite the slight but significant increase of the biliary pressure and ductular proliferation [41]. Therefore, in order to detect adaptive changes in bile secretion developed with time, we considered of interest the comparison of bile secretory events at shorter (24 h) and longer (7-12 days) periods after inducing stenosis.…”

Section: Discussionmentioning

confidence: 96%

“…In the sham-operated rats, the common bile duct was identified and manipulated. Under such conditions the maximal biliary secretion pressure of MSBD rats increased by 13% over that of sham-operated controls [41]. After surgery, the animals were individually housed under the conditions described above.…”

Section: Animal Groupsmentioning

confidence: 99%

“…Under these conditions, practically no changes were observed in distal bile secretion collected 7-10 days postoperatively, with a limited repercussion of humoral effects despite a small increase of biliary pressure and ductular proliferation [41]. Therefore, the model was considered useful to investigate the adaptive mechanisms that regulate bile secretion, as well as to understand the scarcity of symptoms in strictures or compressions of the common bile duct in man, which are seen rather frequently but unpredictably [5,33].…”

Section: Introductionmentioning

confidence: 99%

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Adaptive hepatic changes in mild stenosis of the common bile duct in the rat

et al. 1998

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Adaptive hepatic changes were investigated in rats with mild stenosis of the common bile duct and in sham-operated controls. The studies were performed 24 h and 7-12 days postoperatively. A continuous intravenous infusion of taurocholic acid at stepwise-increasing rates was performed to explore the responses to bile acid effects. During the infusion, bile flow and the outputs of bile acids, phospholipids, cholesterol, alkaline phosphatase and gamma glutamyl transpeptidase were studied. At the end of the infusion, hepatic morphometric measurements were performed. In other experimental sets, biliary excretions of horseradish peroxidase, a marker of microtubule-dependent vesicular transport in the hepatocyte, and sulphobromophthalein, a well-known organic anion model, were studied. In other rats, bile acid pool size and composition were determined by depletion of bile. The results in rats with mild stenosis maintained for 24 h showed a greater susceptibility to the toxicity of taurocholic acid, as revealed by the abrupt decrement in bile flow at high rates of infusion, and increased outputs of phospholipids and canalicular enzymes. Conversely, rats with mild stenosis maintained for 7-12 days showed decreased bile acid maximum secretory rate and biliary outputs of phospholipids and canalicular enzymes, as well as hepatocyte hypertrophy. These findings may explain the limited hepatic and systemic repercussion of experimental mild stenosis of the common bile duct and help us to understand the early stages of constriction of the common bile duct in man.

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Section: Discussionmentioning

confidence: 96%

Section: Animal Groupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adaptive hepatic changes in mild stenosis of the common bile duct in the rat

et al. 1998

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“…Bile duct ligation (BDL) is the most common surgery-based model that induces biliary fibrosis and cholestatic injury, which is based on the double ligation of the bile duct (79). In this way, increased biliary pressure is generated together with inflammation and cytokine secretion, resulting in cholestasis and liver damage.…”

Section: Surgery-based Modelsmentioning

confidence: 99%

Liver fibrosis

Aydin¹,

Akçalı²

2018

Turk J Gastroenterol

291

175

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“…After median laparotomy, the common bile duct was exposed. Mild stenosis of the common bile duct was achieved according to a method described by RodriguezGaray et al [22]. Briefly, a polyethylene catheter (Implantofix, Braun, Melsungen, Germany) was placed along the common bile duct, a double ligature was placed and the catheter removed.…”

Section: Methodsmentioning

confidence: 99%

Long‐Term Evaluation of a Rat Model of Chronic Cholangitis Resembling Human Primary Sclerosing Cholangitis

Goetz

Lehr²,

Galle

et al. 2003

Scand J Immunol

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Primary sclerosing cholangitis (PSC) is a chronic cholestatic disorder with a presumed autoimmune aetiopathogenesis. We have recently described a novel organspecific rat model of fibrosing cholangitis induced by intrabiliary administration of the hapten-reagent 2,4,6-trinitrobenzenesulfonic acid (TNBS) with similarities to human PSC. In the present report, we have evaluated the long-term outcome of TNBS-induced cholangitis in this model. Mild stenosis of the common bile duct of female Lewis rats (n ¼ 18) was achieved by subtotal ligation and cholangitis induced by TNBS injection (50 mg/kg) into the dilated bile duct after a second laparotomy. After 8 and 12 months, we found no evidence of cholangitis in serum chemistry or histology or retrograde cholangiography of TNBS-treated rats. Antineutrophil cytoplasmic antibodies were positive in 75% of animals but were not predictive of liver damage. Tumour necrosis factor-a levels were not elevated in serum or in mononuclear spleen cell supernatants. Our findings suggest that a single initial insult is not sufficient to trigger chronic progressive inflammation. Rather, perpetuation of inflammation probably requires additional stimuli.

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Rat model of mild stenosis of the common bile duct

Cited by 12 publications

References 21 publications

Adaptive hepatic changes in mild stenosis of the common bile duct in the rat

Adaptive hepatic changes in mild stenosis of the common bile duct in the rat

Liver fibrosis

Long‐Term Evaluation of a Rat Model of Chronic Cholangitis Resembling Human Primary Sclerosing Cholangitis

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