Rat islet culture in serum-free medium containing silk protein sericin

Morikawa, Mitsuhiro; Kimura, Toshihisa; Murakami, Makoto; Katayama, Kanji; Terada, Satoshi; Yamaguchi, Akio

doi:10.1007/s00534-009-0049-y

Cited by 31 publications

(24 citation statements)

References 18 publications

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“…Morikawa et al [123] used sericin or FBS (fetal bovine serum) as medium for rat islets culture and there were no observed significant differences in survival and insulin secretion for 14 days. Ogawa et al [83] tested the effect of sericin and FBS on islet cell survival and insulin production and, compared to Morikawa et al [123], on the 12th day, all islets were morphologically intact in FBS culture, while only 50% were intact in sericin culture.…”

Section: Sericin Properties and Biomedical Applicationsmentioning

confidence: 99%

Silkworm Sericin: Properties and Biomedical Applications

Kunz

Brancalhão

Ribeiro

et al. 2016

BioMed Research International

315

261

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Silk sericin is a natural polymer produced by silkworm, Bombyx mori, which surrounds and keeps together two fibroin filaments in silk thread used in the cocoon. The recovery and reuse of sericin usually discarded by the textile industry not only minimizes environmental issues but also has a high scientific and commercial value. The physicochemical properties of the molecule are responsible for numerous applications in biomedicine and are influenced by the extraction method and silkworm lineage, which can lead to variations in molecular weight and amino acid concentration of sericin. The presence of highly hydrophobic amino acids and its antioxidant potential make it possible for sericin to be applied in the food and cosmetic industry. The moisturizing power allows indications as a therapeutic agent for wound healing, stimulating cell proliferation, protection against ultraviolet radiation, and formulating creams and shampoos. The antioxidant activity associated with low digestibility of sericin that expands the application in the medical field, such as antitumour, antimicrobial and anti-inflammatory agent, anticoagulant, acts in colon health, improving constipation and protects the body from obesity through improved plasma lipid profile. In addition, the properties of sericin allow its application as a culture medium and cryopreservation, in tissue engineering and for drug delivery, demonstrating its effective use, as an important biomaterial.

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Section: Sericin Properties and Biomedical Applicationsmentioning

confidence: 99%

Silkworm Sericin: Properties and Biomedical Applications

Kunz

Brancalhão

Ribeiro

et al. 2016

BioMed Research International

315

261

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show abstract

“…In this study, the morphology of pancreatic islets was maintained during isolation by adding sericin to the isolation solution, and Morikawa et al (2009) and Ohnishi et al (2012) reported the maintenance of islets during culture and cryopreservation by the addition of sericin to culture medium and frozen liquid. The advantages of the addition of sericin during the isolation process and the use of sericin in cultures and cryopreservation in the preservation process could be to maintain the morphology of pancreatic islets and to increase the volume of transplantable pancreatic islets isolated from one donor.…”

Section: Discussionmentioning

confidence: 90%

“…There are numerous reports on the various qualities of sericin; it has anti-oxidant and anti-apoptotic properties, is protective against ultraviolet damage, can accelerate the proliferation of various cells, and provide cytoprotective effects (Sasaki et al 2000; Sato et al 2011; Takahashi et al 2003; Terada et al 2002; Zhaorigetu et al 2003, 2007). We previously performed culture preservation and cryopreservation of pancreatic islets to demonstrate the cytoprotective effect of sericin on pancreatic islets (Morikawa et al 2009; Ohnishi et al 2012). …”

Section: Introductionmentioning

confidence: 99%

“…In this study, we defined “isolation” as the process of isolating and purifying islets, as described in the first and second steps above. Morikawa et al (2009) and Onishi et al (2012) reported the effects of sericin in the preservation process; Morikawa et al (2009) added sericin to culture medium, while Onishi et al (2012) added it to frozen liquid. The objective of this study was to determine whether adding sericin to the isolation solution increased the quantity of pancreatic islets isolated.…”

Section: Introductionmentioning

confidence: 99%

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Sericin in the isolating solution improves the yield of islets isolated from the pancreas

et al. 2016

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Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet transplantation by increasing the yield of islets from the pancreas, both the number of islets and their size. Therefore, we used a sericin-containing isolating solution. Rat pancreatic islets were isolated by collagenase digestion and hand picking. We refer to islets isolated with or without sericin in the isolation solution as the sericin and control group, respectively. Volume yield, endocrine function, and islet morphology were compared between the groups. Histological distribution of sericin was evaluated by immunofluorescence staining to examine its mechanism of action in pancreatic islets. The pancreatic islet yield in the sericin group was significantly higher than that in the control group. The endocrine function of islets in the sericin group was comparable to that of islets isolated by conventional methods. Sericin adhered to the surface of isolated pancreatic islets and colocalized with E-cadherin, a cell membrane protein, which might explain the cytoprotective effects of sericin. The islet morphology tended to be better preserved in the sericin group. Sericin could prevent cytoarchitectural damage during the isolation and purification process, resulting in increased pancreatic islet yield. This suggests that sericin could contribute to islet therapy by enhancing the stability of islets.

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“…이러한 세리신은 구조적으로 크게 α-세리신과 β-세리신으로 구분되며, 용해도는 α-세리신이 β-세리신보다 우수한 것으로 알려져 있다. 실크의 천연 단백질은 생체적합성 및 기계적 물성이 우 수하며, 항산화 (Hwang et al 2011), 보습 (Hwang, et al 2009), 미백 (Jo et al 2011(Jo et al , kato et al 1998, 항노화 (Kim et al 2012, Mondal et al 2007) 및 세포사멸억제 (Morikawa et al 2009, Ohnishi et al 2012)와 관련된 효과가 우수한 것으로 알려져 있다 (Prasong et al 2009). 누에를 이용한 생체소재의 개발은 매우 오래전부터 이 루어져 왔다.…”

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Development of functional cosmetic powder using silkgland powder of silkworm

Chon¹,

Kweon²,

Jo³

et al. 2013

Korean Journal of Sericultural Science

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In this study, silkgland powder of silkworm were investigated to see possibility for cosmetic powder materials. To test possibility as a baby powder cosmetics, total content rate of amino acids, DPPH free radical scavenging assay, MTT assay, and clinical trial were done. According to the result of the analysis of the amino acids of silkgland powder, serin (26.77%) content was the highest and asparatic acid (15.47%), and glycine (9.62%) were followed. DPPH free radical scavenging activity of silkgland powder was lower than vitamin C by 82.3% and 97%, respectively, which is relatively good. And the moisture and elasticity effect were increased in silkgland powder compared to control cosmetics by 210% and 185%, relatively. Thus, these results suggest that silkgland powder of silkworm may have beneficial properties as a material for cosmeceuticals.

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Rat islet culture in serum-free medium containing silk protein sericin

Abstract: Serum-free medium containing sericin appears to be useful for islet culture.

Cited by 31 publications

References 18 publications

Silkworm Sericin: Properties and Biomedical Applications

Silkworm Sericin: Properties and Biomedical Applications

Sericin in the isolating solution improves the yield of islets isolated from the pancreas

Development of functional cosmetic powder using silkgland powder of silkworm

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