Rat hepatocyte cultures and co-cultures in biotransformation studies of xenobiotics

Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes

“…However, in rat hepatocytes, phase II enzymes in general showed a decrease in gene expression level after 72 h compared with gene expression in liver (Kienhuis et al, 2007). For rat hepatocytes, it is known that the phase II enzymes are better preserved in culture than phase I enzymes (Rogiers and Vercruysse, 1993;Kern et al, 1997). Our data confirm these findings, thus showing that mouse hepatocytes are also stable in expression of genes encoding phase II enzymes.…”

Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes

“…However, in rat hepatocytes, phase II enzymes in general showed a decrease in gene expression level after 72 h compared with gene expression in liver (Kienhuis et al, 2007). For rat hepatocytes, it is known that the phase II enzymes are better preserved in culture than phase I enzymes (Rogiers and Vercruysse, 1993;Kern et al, 1997). Our data confirm these findings, thus showing that mouse hepatocytes are also stable in expression of genes encoding phase II enzymes.…”

Effect of the Histone Deacetylase Inhibitor Trichostatin A on the Metabolome of Cultured Primary Hepatocytes

“…We isolated the aqueous intracellular metabolites from control and TSA treated primary cultured hepatocytes using a methanol/chloroform extraction (as described in methods section). The aqueous intracellular fractions were subjected to 1 H NMR spectroscopic and chemometric analysis.…”

Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow

“…The potential to induce CYP-dependent monooxygenases is considered as one of the most representative functional parameters to evaluate the adult hepatic phenotype [22,23]. Therefore, the responsiveness of both CYP1A1/2 and CYP2B6 to their respective prototype inducers 3-methylcholantrene (MC) and phenobarbital (PB) was studied in parallel.…”

Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes

“…However, in rat hepatocytes, phase II enzymes in general showed a decrease in gene expression level after 72 h compared with gene expression in liver (Kienhuis et al, 2007). For rat hepatocytes, it is known that the phase II enzymes are better preserved in culture than phase I enzymes (Rogiers and Vercruysse, 1993;Kern et al, 1997). Our data confirm these findings, thus showing that mouse hepatocytes are also stable in expression of genes encoding phase II enzymes.…”

Effect of the Histone Deacetylase Inhibitor Trichostatin A on the Metabolome of Cultured Primary Hepatocytes

“…We isolated the aqueous intracellular metabolites from control and TSA treated primary cultured hepatocytes using a methanol/chloroform extraction (as described in methods section). The aqueous intracellular fractions were subjected to 1 H NMR spectroscopic and chemometric analysis.…”

Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow

“…The potential to induce CYP-dependent monooxygenases is considered as one of the most representative functional parameters to evaluate the adult hepatic phenotype [22,23]. Therefore, the responsiveness of both CYP1A1/2 and CYP2B6 to their respective prototype inducers 3-methylcholantrene (MC) and phenobarbital (PB) was studied in parallel.…”

Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes

“…However, in rat hepatocytes, phase II enzymes in general showed a decrease in gene expression level after 72 h compared with gene expression in liver (Kienhuis et al, 2007). For rat hepatocytes, it is known that the phase II enzymes are better preserved in culture than phase I enzymes (Rogiers and Vercruysse, 1993;Kern et al, 1997). Our data confirm these findings, thus showing that mouse hepatocytes are also stable in expression of genes encoding phase II enzymes.…”

Effect of the Histone Deacetylase Inhibitor Trichostatin A on the Metabolome of Cultured Primary Hepatocytes

“…We isolated the aqueous intracellular metabolites from control and TSA treated primary cultured hepatocytes using a methanol/chloroform extraction (as described in methods section). The aqueous intracellular fractions were subjected to 1 H NMR spectroscopic and chemometric analysis.…”

Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow

“…The potential to induce CYP-dependent monooxygenases is considered as one of the most representative functional parameters to evaluate the adult hepatic phenotype [22,23]. Therefore, the responsiveness of both CYP1A1/2 and CYP2B6 to their respective prototype inducers 3-methylcholantrene (MC) and phenobarbital (PB) was studied in parallel.…”