Rat gastric mucosal cAMP following cholinergic and histamine stimulation

Evidence for involvement of cyclic nucleotides in intrinsic factor secretion by isolated rabbit gastric mucosa.

“…We have now investigated the possibility that cyclic nucleotides may play a role in IF secretion. A phosphodiesterase inhibitor, 3-isobutyl methylxanthine (IBMX), stimulated IF secretion nearly fourfold while increasing tissue levels of both cyclic AMP (cAMP) and cyclic GMP (cGMP). Peak IF secretion in response to IBMX was not reached until after tissue cAMP levels were maximal.…”

“…Since Kimberg's comprehensive review (1) appeared in 1974, the results of numerous investigations have argued for (2)(3)(4)(5)(6)(7) and against (8,9) an important role for cyclic nucleotides in mammalian gastric secretion. These investigations have been concerned with hydrochloric acid secretion (6,8) and with adenylate cyclase activity and levels of adenosine 3,5'-cyclic AMP (cAMP)l in gastric mucosa (2)(3)(4)(5)(7)(8)(9).…”

“…These investigations have been concerned with hydrochloric acid secretion (6,8) and with adenylate cyclase activity and levels of adenosine 3,5'-cyclic AMP (cAMP)l in gastric mucosa (2)(3)(4)(5)(7)(8)(9). We are unaware of studies on the possible relationships between cyclic nucleotides and gastric secretion of macromolecules.…”

Hydrochloric Acid and Cyclic 3’.5’-Adenosine Monophosphate Content of Rodent Gastric Juice after Halothane Administration

“…The literature is replete with reports that cAMP may play a role in gastric acid secretion in different species, including rats (5,12,19,33), amphibians (17,26), guinea pigs (28), rabbits (14,36), dogs (7) and man (7,8). Bieck et al (7) reported that histamine and pentagastrin increased cAMP levels in both gastric mucosa and gastric juice prior to the onset of acid secretion in dog and man.…”

“…The injection of pentagastrin or histamine in rats in doses that stimulated acid secretion increased adenylase activity and cAMP content in gastric tissue (5,12,19,33). Since halothane is one of the most commonly administered volatile anesthetic agents, the influence of different doses of halothane (5, 10 and 20 mg/kg intra venously) on gastric acid secretion and cAMP content in pyloric and cardiac ligated rats was investigated.…”