Rat Connexins 30.3 and 31 Are Expressed in the Kidney

Tucker, Mark A.; Barajas, Luciano

doi:10.1006/excr.1994.1194

Cited by 24 publications

(11 citation statements)

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“…Nine connexins have been recognized in the kidney namely, Cx26, Cx30.3, Cx31, Cx32, Cx37, Cx40, Cx43, Cx45, and Cx46 (Paul 1986;Zhang and Nicholson 484 Fig. In A, the transition in the staining between the media and the endothelium is not apparent due to orientation of the arteriole 1989; Paul et al 1991;Haefliger et al 1992;Hennemann et al 1992;Barajas et al 1994;Tucker and Barajas 1994;Hillis et al 1997;Guo et al 1998;Seul and Beyer 2000). Cx40 immunoreactivity occurred in the media of the juxtaglomerular part of the afferent arteriole and in mesangial cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles

Arensbak

Mikkelsen

Gustafsson

et al. 2001

Histochem Cell Biol

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Gap junctions allow direct intercellular coupling between many cells including those in the vascular wall. Studies of connexin expression in cells of the microcirculatory system are very few in number. However, cell-to-cell communication between cells of the arteriolar wall may be particularly important in microcirculatory control. We investigated the expression of connexins 43, 40, and 37 (Cx43, Cx40, Cx37) mRNA and proteins in primary cultures of smooth muscle cells (SMC) from rat renal preglomerular arterioles and in the aortic cell line A7r5. Furthermore protein expression in preglomerular arterioles in frozen sections was evaluated. SMC were isolated from kidneys using an iron oxide sieve method and explant technique. Total RNA from these cultures was tested by RT-PCR analysis for the expression of the three connexins mRNA. Using immunofluorescence we examined whether the expression pattern of connexin protein in the cell culture and frozen sections corresponded to the mRNA expression. The data show that A7r5 and preglomerular SMC express mRNA for Cx37 in addition to Cx43 and Cx40. In A7r5 cells the mRNA for Cx43, Cx40, and Cx37 are translated to protein, whereas cultured preglomerular SMC and the media of afferent arterioles in frozen sections only showed Cx40 immunoreactivity.

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Section: Discussionmentioning

confidence: 99%

Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles

Arensbak

Mikkelsen

Gustafsson

et al. 2001

Histochem Cell Biol

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“…GJB3 and GJB5 encoding the putative proteins Cx31 and Cx31.1, respectively, belong to the b-Cx family, and were detected in human epidermis by qualitative RT-PCR from total epidermal RNA, immunostaining and/or Northern blotting (23,110). Cx31 transcripts were also detected in skin, testis, kidney, placenta, and ear (22,58,(110)(111)(112). The predicted protein structure of both gap junctional proteins is similar to other Cx, including a conserved arrangement of 3 cysteine residues in each extracellular loop.…”

Section: Connexin Disorders Of the Skin Cx31 Defects In Erythrokeratomentioning

confidence: 99%

Connexins: a connection with the skin

Richard

2000

Experimental Dermatology

162

112

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The intercellular signaling system mediated by connexin chanInstitute of Molecular Medicine, Thomas nels is crucial for maintaining tissue homeostasis, growth control, developJefferson University, Philadelphia, PA, USA ment, and synchronized response of cells to stimuli. This review summarizes the structure, assembly, and properties of the components of the complex and diverse connexin system, and their biological functions in

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“…Thus, mutations within the M3 domain may produce different phenotypes, such as hearing or skin disorders. This is not surprising, since Cx30.3 mRNA expression is restricted to skin and kidney in rodents, whereas the gene for Cx31 (GJB3) is also expressed in the inner ear and in the CNS (Hoh et al 1991;Hennemann et al 1992;Tucker and Barajas 1994).…”

Section: Figurementioning

confidence: 99%

Mutation in the Gene for Connexin 30.3 in a Family with Erythrokeratodermia Variabilis

Macari¹,

Landau²,

Cousin³

et al. 2000

Am. J Hum. Genet.

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Erythrokeratodermia variabilis (EKV) is an autosomal dominant keratinization disorder characterized by migratory erythematous lesions and fixed keratotic plaques. All families with EKV show mapping to chromosome 1p34-p35, and mutations in the gene for connexin 31 (Cx31) have been reported in some but not all families. We studied eight affected and three healthy subjects in an Israeli family, of Kurdish origin, with EKV. After having mapped the disorder to chromosome 1p34-p35, we found no mutations in the genes for Cx31, Cx31.1, and Cx37. Further investigation revealed a heterozygous TrC transition leading to the missense mutation (F137L) in the human gene for Cx30.3 that colocalizes on chromosome 1p34-p35. This nucleotide change cosegregated with the disease and was not found in 200 alleles from normal individuals. This mutation concerns a highly conserved phenylalanine, in the third transmembrane region of the Cx30.3 molecule, known to be implicated in the wall formation of the gap-junction pore. Our results show that mutations in the gene for Cx30.3 can be causally involved in EKV and point to genetic heterogeneity of this disorder. Furthermore, we suggest that our family presents a new type of EKV because of the hitherto unreported association with erythema gyratum repens.Connexins (denoted by the prefix "Cx") are a family of polypeptides that form the subunits of the gap-junction channels. Members of the connexin family are characterized by four hydrophobic transmembrane domains (M1-M4) that are linked by one cytoplasmic and two extracellular (E1 and E2) loops. The N and C termini are located on the cytoplasmic membrane face. Extracellular-loop and transmembrane domains display the highest homology between the connexin family members, whereas the cytoplasmic loop and the C-terminal region are highly variable. Six connexin polypeptides assemble into a connexon, a hemichannel that interacts with its counterpart on adjacent cells to form a complete intercellular channel, thereby connecting the cytoplasm of neighboring cells (Yeager and Nicholson 1996). Gap junctions are composed of numerous aggregated con-

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Rat Connexins 30.3 and 31 Are Expressed in the Kidney

Cited by 24 publications

References 0 publications

Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles

Expression of connexin 37, 40, and 43 mRNA and protein in renal preglomerular arterioles

Connexins: a connection with the skin

Mutation in the Gene for Connexin 30.3 in a Family with Erythrokeratodermia Variabilis

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