Rat chondrocyte proliferation was regulated by natriuretic peptide C and its receptor, natriuretic peptide receptor-B, in autocrine manner

Hagiwara, Hiroaki; Sakaguchi, H; Itakura, Mitsuo; Inoue, Atsuto; Yoshimoto, T.; Furuya, Masako; Tanaka, Sayoko; Hirose, Shintaro

doi:10.1016/0928-4680(94)90979-2

Cited by 48 publications

(60 citation statements)

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“…Interestingly, cGMP accumulation in response to maximal concentrations of all three ligands was significantly greater in aT3-1 cells compared with LbT2 cells ( Fig. 5D; As several studies have reported a potent anti-proliferative effect of CNP (Hagiwara et al 1994, Tao et al 1999, Simpson et al 2002, we next examined whether CNP could alter proliferation or cell cycle distribution in aT3-1 or LbT2 cells. Cells were plated in low-serum medium (2% v/v) for 48 h in the absence or presence of the indicated concentrations of either CNP or GNRH prior to cell counting or FACS analyses.…”

Section: Functional Effects Of Natriuretic Peptides In Gonadotroph Cementioning

confidence: 99%

Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, αT3-1 and LβT2 gonadotroph cells

Thompson

Chand²,

Jonas

et al. 2009

Journal of Endocrinology

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In the pituitary, C-type natriuretic peptide (CNP) has been implicated as a gonadotroph-specific factor, yet expression of the CNP gene (Nppc) and CNP activity in gonadotrophs is poorly defined. Here, we examine the molecular expression and putative function of a local gonadotroph natriuretic peptide system. Nppc, along with all three natriuretic peptide receptors (Npr1, Npr2 and Npr3), was expressed in both aT3-1 and LbT2 cells and primary mouse pituitary tissue, yet the genes for atrial-(ANP) and B-type natriuretic peptides (Nppa and Nppb) were much less abundant. Putative processing enzymes of CNP were also expressed in aT3-1 cells and primary mouse pituitaries. Transcriptional analyses revealed that the proximal 50 bp of the murine Nppc promoter were sufficient for GNRH responsiveness, in an apparent protein kinase C and calcium-dependent manner. Electrophoretic mobility shift assays showed Sp1/Sp3 proteins form major complexes within this region of the Nppc promoter. CNP protein was detectable in rat anterior pituitaries, and electron microscopy detected CNP immunoreactivity in secretory granules of gonadotroph cells. Pharmacological analyses of natriuretic peptide receptor activity clearly showed ANP and CNP are potent activators of cGMP production. However, functional studies failed to reveal a role for CNP in regulating cell proliferation or LH secretion. Surprisingly, CNP potently stimulated the human glycoprotein hormone a-subunit promoter in LbT2 cells but not in aT3-1 cells. Collectively, these findings support a role for CNP as the major natriuretic peptide of the anterior pituitary, and for gonadotroph cells as the major source of CNP expression and site of action.

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Section: Functional Effects Of Natriuretic Peptides In Gonadotroph Cementioning

confidence: 99%

Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, αT3-1 and LβT2 gonadotroph cells

Thompson

Chand²,

Jonas

et al. 2009

Journal of Endocrinology

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“…CNP is certainly a neuropeptide that is synthesized in largest quantities in the brain, but its central actions, such as its antidipsogenic effect and the inhibition of AVP secretion, are much less potent than ANP and BNP. In the periphery, CNP seems to be involved in vascular remodeling and in bone/cartilage formation through its action as an anti-growth factor (32,39,79). CNP may act locally in Fig.…”

Section: Anp Is a Volume-regulating Hormone In Mammalsmentioning

confidence: 99%

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

Takei

Hirose

2002

American Journal of Physiology-Regulatory, Integrative and Comp

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Takei, Yoshio, and Shigehisa Hirose. The natriuretic peptide system in eels: a key endocrine system for euryhalinity? Am J Physiol Regulatory Integrative Comp Physiol 282: R940-R951, 2002; 10.1152/ajpregu. 00389.2001.-The natriuretic peptide system of a euryhaline teleost, the Japanese eel (Anguilla japonica), consists of three types of hormones [atrial natriuretic peptide (ANP), ventricular natriuretic peptide (VNP), and Ctype natriuretic peptide (CNP)] and four types of receptors [natriuretic peptide receptors (NPR)-A, -B, -C, and -D]. Although ANP is recognized as a volume-regulating hormone that extrudes both Na ϩ and water in mammals, ANP more specifically extrudes Na ϩ in eels. Accumulating evidence shows that ANP is secreted in response to hypernatremia and acts to inhibit the uptake and to stimulate the excretion of Na ϩ but not water, thereby promoting seawater (SW) adaptation. In fact, ANP is secreted immediately after transfer of eels to SW and ameliorates sudden increases in plasma Na ϩ concentration through inhibition of drinking and intestinal absorption of NaCl. ANP also stimulates the secretion of cortisol, a long-acting hormone for SW adaptation, whereas ANP itself disappears quickly from the circulation. Thus ANP is a primary hormone responsible for the initial phase of SW adaptation. By contrast, CNP appears to be a hormone involved in freshwater (FW) adaptation. Recent data show that the gene expression of CNP and its specific receptor, NPR-B, is much enhanced in FW eels. In fact, CNP infusion increases 22 Na uptake from the environment in FW eels. These results show that ANP and CNP, despite high sequence identity, have opposite effects on salinity adaptation in eels. This difference apparently originates from the difference in their specific receptors, ANP for NPR-A and CNP for NPR-B. VNP may compensate the effects of ANP and CNP for adaptation to respective media, because it has high affinity to both receptors. On the basis of these data, the authors suggest that the natriuretic peptide system is a key endocrine system that allows this euryhaline fish to adapt to diverse osmotic environments, particularly in the initial phase of adaptation. atrial natriuretic peptide; ventricular natriuretic peptide; C-type natriuretic peptide; membrane guanylyl cyclase; water and electrolyte balance; molecular evolution; functional evolution; volume regulation; sodium regulation; Anguilla japonica ATRIAL NATRIURETIC PEPTIDE (ANP) was the first member of the natriuretic peptide (NP) family identified in mammals (17,63,69,86). Subsequent to the isolation and sequencing of ANP from the rat atria in 1983, brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP) were isolated from the porcine brain in 1988 and 1990, respectively, and they were identified as members of the NP family (Fig. 1). Whereas ANP and BNP are circulating hormones secreted from the heart, CNP is principally a paracrine factor in the brain and periphery (71). The basic structure of the NP peptides consists of an intramolecular...

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“…The dwarfism of CNP null mice is completely rescued by targeted over-expression of CNP in the growth plate chondrocytes under the control of the murine pro-al(II) collagen promoter [174]. It has been shown that CNP is secreted from primary cultures of chondrocytes [190] (Fig. 6).…”

Section: F Bnp Transgenic Micementioning

confidence: 98%

The Regulation and Physiological Roles of the Guanylyl Cyclase Receptors.

2001

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Rat chondrocyte proliferation was regulated by natriuretic peptide C and its receptor, natriuretic peptide receptor-B, in autocrine manner

Cited by 48 publications

References 0 publications

Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, αT3-1 and LβT2 gonadotroph cells

Molecular characterisation and functional interrogation of a local natriuretic peptide system in rodent pituitaries, αT3-1 and LβT2 gonadotroph cells

The natriuretic peptide system in eels: a key endocrine system for euryhalinity?

The Regulation and Physiological Roles of the Guanylyl Cyclase Receptors.

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