Rat brain proteome in morphine dependence

Bierczyńska-Krzysik, Anna; Bonar, Emilia; Drabik, Anna; Noga, Marek; Suder, Piotr; Dyląg, Tomasz; Dubin, Adam; Kotlińska, Jolanta; Silberring, Jerzy

doi:10.1016/j.neuint.2006.01.024

Cited by 41 publications

(33 citation statements)

References 40 publications

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“…In this case, the cell culture assures significant simplification of the sample and may allow for observation of low abundance proteins. This feature is clearly visible when we compared results obtained for beta-actin during a morphinome study in the brain [2], striatum [4], nucleus accumbens [5], and on the neuronal striatal cell culture level from our study. Changes in protein quantity are undetectable in morphinome from brain and striatum, probably due to the complexity of the samples.…”

Section: Discussionmentioning

confidence: 60%

“…Due to this action, this protein could also be considered as a chaperone and in some neurodegenerative disorders such as ischemia the elevated level of this protein may play a neuroprotective role [43]. An up-regulated level of this protein was also found after morphine administration in the whole rat brain morphinome study [2].…”

Section: Proteins Involved In Protein Biosynthesismentioning

confidence: 99%

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Proteomic analysis of striatal neuronal cell cultures after morphine administration

Bodzoń‐Kułakowska

Suder²,

Mak

et al. 2009

J of Separation Science

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Using primary neuronal cell culture assays, combined with 2-D gel electrophoresis and capillary LC-MS, we identified differences in proteomes between control and morphine-treated cells. Statistically significant differences were observed among 26 proteins. Nineteen of them were up-regulated, while seven were down-regulated in morphine-treated cell populations. The identified proteins belong to classes involved in energy metabolism, associated with oxidative stress, linked with protein biosynthesis, cytoskeletal ones, and chaperones. The detected proteins demand further detailed studies of their biological roles in morphine addiction. It is crucial to confirm observed processes in vivo in order to reveal the nature and importance of the biological effect of proteome changes after morphine administration. Further investigations may lead to the discovery of new proteome-based effects of morphine on living organisms.

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Section: Discussionmentioning

confidence: 60%

Section: Proteins Involved In Protein Biosynthesismentioning

confidence: 99%

Proteomic analysis of striatal neuronal cell cultures after morphine administration

Bodzoń‐Kułakowska

Suder²,

Mak

et al. 2009

J of Separation Science

Self Cite

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“…Because of their importance in the development and maintenance of drug dependence, a few brain regions, such as the NAc [50], frontal cortex [12,51], hippocampus [12], and striatum [12,52], are the primary targets of proteomics analysis in the study of morphine, heroin, or butorphanol addiction. Whole brain [53] or components of the nervous system, such as the spinal cord [54], dorsal root ganglia [55], or cultured neuronal cells [56,57], also have been investigated. In some studies, proteins expressed in the synapse were extracted and analyzed directly via proteomics approaches [58][59][60][61].…”

Section: Morphine/heroin/butorphanolmentioning

confidence: 99%

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Wang

Yuan

2011

Mol Neurobiol

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Drug addiction is a chronic neuronal disease. In recent years, proteomics technology has been widely used to assess the protein expression in the brain tissues of both animals and humans exposed to addictive drugs. Through this approach, a large number of proteins potentially involved in the etiology of drug addictions have been identified, which provide a valuable resource to study protein function, biochemical pathways, and networks related to the molecular mechanisms underlying drug dependence. In this article, we summarize the recent application of proteomics to profiling protein expression patterns in animal or human brain tissues after the administration of alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine/heroin/butorphanol, or nicotine. From available reports, we compiled a list of 497 proteins associated with exposure to one or more addictive drugs, with 160 being related to exposure to at least two abused drugs. A number of biochemical pathways and biological processes appear to be enriched among these proteins, including synaptic transmission and signaling pathways related to neuronal functions. The data included in this work provide a summary and extension of the proteomics studies on drug addiction. Furthermore, the proteins and biological processes highlighted here may provide valuable insight into the cellular activities and biological processes in neurons in the development of drug addiction.

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“…Recently, 2D-PAGE, in combination with mass spectrometry has been widely used in the identification of specific biochemical markers for diagnostic, preventive and therapeutic intervention in drug addiction, including morphine [11][12][13][14][15], cocaine [16][17][18], nicotine [19,20], heroin [21], alcohol [22,23] and butorphanol [24]. The neurotoxic effects of drug abuse are often associated with oxidative stress, mitochondrial dysfunction, apoptosis and inhibition of neurogenesis, among other mechanisms [25].…”

Section: Introductionmentioning

confidence: 99%