Rat brain guanosine binding site

Traversa, U.; Bombi, Giulia; Camaioni, Emidio; Macchiarulo, Antonio; Costantino, Gabriele; Palmieri, Clara; Caciagli, Francesco; Pellicciari, Roberto

doi:10.1016/j.bmc.2003.09.043

Cited by 62 publications

(33 citation statements)

References 24 publications

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“…Indeed, a G-protein-coupled site seems to be important for the neuroprotective effects of this nucleoside, namely, during the guanosine-induced stimulation of glutamate uptake and the release of trophic factors by astrocytes [39,82,86]. In support of this hypothesis, binding sites for guanosine, distinct from the well-characterized P1 and P2 receptors, were found in rat brain membranes [87][88][89]. In addition, experimental evidence suggests that this binding site has an important role in signal transduction mediated by this nucleoside [39,82,86].…”

Section: Metabolism and Intracellular Signaling Pathways Triggered Bysupporting

confidence: 59%

“…In particular, further elucidation of the interaction between guanosine and the adenosinergic system is warranted. In addition, even though recent evidence has proposed the existence of a putative G-protein-linked cell surface receptor that may be modulating the effects of guanosine [15,82,87,88], such receptor has not yet been cloned and characterized. Answering these questions will certainly enhance our understanding of how guanosine modulates distinct signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, this nucleoside also stimulates the astrocytic release of trophic factors and activates neuroprotective mechanisms including antiapoptotic, antiinflammatory, and antioxidant responses. [94] ↓ Seizures (through a mechanism distinct from that of MK-801) [119] ↓ Oxidative stress; ↑ endogenous antioxidants [87] Stroke (hypoxia/ischemia) In vitro…”

Section: Protective Effects Of Guanosine Against Hypoxia/ischemiamentioning

confidence: 99%

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Guanosine and its role in neuropathologies

Bettio

Gil-Mohapel

Rodrigues

2016

Purinergic Signalling

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Guanosine is a purine nucleoside thought to have neuroprotective properties. It is released in the brain under physiological conditions and even more during pathological events, reducing neuroinflammation, oxidative stress, and excitotoxicity, as well as exerting trophic effects in neuronal and glial cells. In agreement, guanosine was shown to be protective in several in vitro and/or in vivo experimental models of central nervous system (CNS) diseases including ischemic stroke, Alzheimer's disease, Parkinson's disease, spinal cord injury, nociception, and depression. The mechanisms underlying the neurobiological properties of guanosine seem to involve the activation of several intracellular signaling pathways and a close interaction with the adenosinergic system, with a consequent stimulation of neuroprotective and regenerative processes in the CNS. Within this context, the present review will provide an overview of the current literature on the effects of guanosine in the CNS. The elucidation of the complex signaling events underlying the biochemical and cellular effects of this nucleoside may further establish guanosine as a potential therapeutic target for the treatment of several neuropathologies.

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Section: Metabolism and Intracellular Signaling Pathways Triggered Bysupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Protective Effects Of Guanosine Against Hypoxia/ischemiamentioning

confidence: 99%

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Guanosine and its role in neuropathologies

Bettio

Gil-Mohapel

Rodrigues

2016

Purinergic Signalling

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“…However, many of the effects of guanine-based purines persist in the presence of P1 and/or P2 purinoreceptor antagonists 17 . In addition, several of the effects of guanosine may be mediated through G protein-dependent signalling pathways involving cyclic nucleotides or MAP kinase pathway 19 , and a specific binding site for guanosine has been detected on membrane preparations from rat brain 20,21 . Thus, the possibility of the existence of distinct receptors for guaninebased purines has been raised.…”

Section: Theoretical Survey On Tautomerism Of Thioguanine Tautomers Bmentioning

confidence: 99%

Theoretical Survey on Tautomerism of Thioguanine Tautomers by Polarisable Continuum Method (PCM)

Heidarnezhad¹,

Heidarnezhad²,

Ганиев³

et al. 2013

Orientjchem.

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Computational calculations at B3LYP/CC-PVDZ level were employed in the study of tautomers of Thioguanine (TG) in the gas phase and selected solvents such as benzene , tetrahydrofuran (THF), methanol, Dimethyl sulfoxide (DMSO) and water using PCM model. All tautomers are optimized at this level. In addition, stability of the tautomers in different solvents shows interesting results. In the gas phase, benzene and THF, TG1 form is more stable than the other forms but in polarisable solvents (methanol, DMSO and water) TG5 is the most stabilized form. Variation of dipole moments and NBO charges on atoms in the solvents were studied.

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“…The purines and pyrimidines were isolated from CSF by the solid phase extraction (SPE) using Supelco Preppy apparatus (Sigma-Aldrich, Europe) with Strata X column, 30 …”

Section: Apparatusmentioning

confidence: 99%