Rat bradykinin B2 receptor: Immunochemical characterization and immunovizualization in epithelial and smooth muscle cells

Figueroa, Carlos D.; Marchant, Alejandra; Menzel, Doris; Novoa, Ulises; González, Carlos B.; Müller‐Esterl, Werner

doi:10.1016/0162-3109(96)00016-1

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…B2 receptors are shown to be preferentially expressed in the distal nephron including cortical and medullary collecting duct (47,59). Here, B2 receptors are localized to both apical and basolateral membranes (13,14). Thus, locally generated kinins from both luminal and interstitial sides are able to stimulate B2 receptors.…”

Section: Discussionmentioning

confidence: 94%

Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron

Zaika

Mamenko

O’Neil

et al. 2011

American Journal of Physiology-Renal Physiology

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Activation of the renal kallikrein-kinin system results in natriuresis and diuresis, suggesting its possible role in renal tubular sodium transport regulation. Here, we used patch-clamp electrophysiology to directly assess the effects of bradykinin (BK) on the epithelial Na(+) channel (ENaC) activity in freshly isolated split-opened murine aldosterone-sensitive distal nephrons (ASDNs). BK acutely inhibits ENaC activity by reducing channel open probability (P(o)) in a dose-dependent and reversible manner. Inhibition of B2 receptors with icatibant (HOE-140) abolished BK actions on ENaC. In contrast, activation of B1 receptors with the selective agonist Lys-des-Arg(9)-BK failed to reproduce BK actions on ENaC. This is consistent with B2 receptors playing a critical role in mediating BK signaling to ENaC. BK has little effect on ENaC P(o) when G(q/11) was inhibited with Gp antagonist 2A. Moreover, inhibition of phospholipase C (PLC) with U73122, but not saturation of cellular cAMP levels with the membrane-permeable nonhydrolysable cAMP analog 8-cpt-cAMP, prevents BK actions on ENaC activity. This argues that BK stimulates B2 receptors with subsequent activation of G(q/11)-PLC signaling cascade to acutely inhibit ENaC activity. Activation of BK signaling acutely depletes apical PI(4,5)P(2) levels. However, inhibition of Ca(2+) pump SERCA of the endoplasmic reticulum with thapsigargin does not prevent BK signaling to ENaC. Furthermore, caffeine, while producing a similar rise in [Ca(2+)](i) as in response to BK stimulation, fails to recapitulate BK actions on ENaC. Therefore, we concluded that BK acutely inhibits ENaC P(o) in mammalian ASDN via stimulation of B2 receptors and following depletion of PI(4,5)P(2), but not increases in [Ca(2+)](i).

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Section: Discussionmentioning

confidence: 94%

Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron

Zaika

Mamenko

O’Neil

et al. 2011

American Journal of Physiology-Renal Physiology

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Tissue Kallikrein–Kinin System

Clements

2000

Comprehensive Physiology

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The sections in this article are: Historical Perspectives Kallikrein–Kinin Systems Plasma Kallikrein–Kinin System Tissue Kallikrein–Kinin System Components of the Tissue Kallikrein–Kinin System Tissue Kallikrein Kallikrein Inhibitors: Kallistatin Kininogens Kinins and Peptidase Metabolism Kinin Receptors Tissue Kallikrein–Kinin System and Electrolyte and Water Balance in the Kidney Localization of Kallikrein–Kinin System Components Interrelationship of Renin–Angiotensin and Kallikrein–Kinin Systems Regulatory and Mediatory Factors Kinins and Electrolyte Transport Prostaglandins and Other Intracellular Mediators Tissue Kallikrein–Kinin System and Electrolyte Transport in Other Tissues Colon and Intestinal Epithelia Other Epithelia Overview

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Intracarotid low dose bradykinin infusion selectively increases tumor permeability through activation of bradykinin B2 receptors in malignant gliomas

1998

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Rat bradykinin B2 receptor: Immunochemical characterization and immunovizualization in epithelial and smooth muscle cells

Cited by 5 publications

References 17 publications

Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron

Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron

Tissue Kallikrein–Kinin System

Intracarotid low dose bradykinin infusion selectively increases tumor permeability through activation of bradykinin B2 receptors in malignant gliomas

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Rat bradykinin B2 receptor: Immunochemical characterization and immunovizualization in epithelial and smooth muscle cells

Cited by 5 publications

References 17 publications

Bradykinin acutely inhibits activity of the epithelial Na+channel in mammalian aldosterone-sensitive distal nephron

Bradykinin acutely inhibits activity of the epithelial Na+channel in mammalian aldosterone-sensitive distal nephron

Tissue Kallikrein–Kinin System

Intracarotid low dose bradykinin infusion selectively increases tumor permeability through activation of bradykinin B2 receptors in malignant gliomas

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Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron

Bradykinin acutely inhibits activity of the epithelial Na⁺channel in mammalian aldosterone-sensitive distal nephron