Rat bone marrow mesenchymal stem cells differentiate into hepatocytesin vitro

Abstract

The method combining gradient density centrifugation with plastic adherence can isolate MSCs. Rat MSCs may be differentiated into hepatocytes by FGF-4 and HGF. Cytokines may play a more important role in differentiation from rat MSCs into hepatocytes.

Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes

“…Schwartz et al [14] . and Kang et al [15] . reported that FGF-4 and HGF could induce differentiation of bone marrow MSCs into functional hepatocyte-like cells.…”

“…It has been reported that bone marrowderived MSCs can differentiate into mesoderm cells such as osteoblasts [1][2][3] , adipocytes [4][5][6] , neural and brain cells [7][8][9][10] ,cardiomyocytes [11][12][13] and hepatocytes [14,15] in vivo or in vitro. However, it has been demonstrated that the number and the differentiating potential of bone marrow MSCs decrease with age [16].…”

A Comparative Study of Proliferation and Hepatic Differentiation of Human Adipose-Derived Stem Cells

“…One medium (medium A) was supplemented with hepatocyte growth factor (HGF), a mesenchymally derived potent mitogen for parenchymal hepatocyte cells which acts via the HGF receptor c-MET, a transmembrane tyrosine kinase protein [Schaffler and Buchler, 2007]. As is well known, stem cells derived from human liver and rat bone marrow differentiate in mature hepatocytes when cultured in vitro in the presence of HGF and fibroblast growth factor 4, as indicated by the expression of functional cytochrome P450, albumin and urea production [Kang et al, 2005;Herrera et al, 2006]. In the second procedure, the me dium (medium B) was supplemented with oncostatin M (OSM), basic fibroblast growth factor (bFGF) and dimethyl sulfoxide (DMSO).…”

Liver Parenchyma Regeneration in Connection with Extended Surgical Procedure - Experiment on Large Animal

“…Most of these approaches used toxic models, in which the intrinsic selfrenewing capacity of the recipient liver was suppressed (Dahlke, 2003, Kang, 2005. Di Bonzo observed only 0,2% human hepatocytes after transplantation of human MSC into immunodeficient mice with chronic liver injury (Di Bonzo, 2008).…”