Rat atrial natriuretic peptide elevates the level of cyclic GMP in astroglia-rich brain cell cultures

Friedl, Arno; Harmening, Christine; Schuricht, Barbara; Hamprecht, Bernd

doi:10.1016/0014-2999(85)90126-8

Cited by 38 publications

(10 citation statements)

References 3 publications

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“…The presence of ANF as well as its binding sites has recently been reported in various regions of brain (Quirion et al, 1984(Quirion et al, , 1986Stone and Glembotski, 1985). ANF has also been shown to stimulate the cyclic GMP level in cultured brain cells (Fried1 et al, 1985) and in brain slice preparations (Takayanagi et al, 1986). These findings have suggested that ANF may play a regulatory role in the CNS and are compatible with the present findings of the stimulation of intracellular cyclic GMP level by ANF and the presence of ANF receptor in these cultured neuroblastoma cells of ganglionic origin.…”

Section: Discussionmentioning

confidence: 88%

entaaification of Atrial Natriuretic Factor Receptor of Neuroblastoma N4TG1 Cells: Binding Characteristics and Photoaffinity Labeling

Pandey

Misono

Takayanagi

et al. 1987

Journal of Neurochemistry

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We have found specific receptors for atrial natriuretic factor (ANF) in cultured neuroblastoma cells (N4TG1) of peripheral ganglionic origin. Scatchard analysis of the displacement binding revealed noninteracting, single-class binding sites with a KD of 1 X 10(-10) M and a density (Bmax) of 110,000-150,000 sites/cell. The cell-bound 125I-ANF was displaced by unlabeled ANF in a dose-dependent manner. Hormones unrelated to ANF such as angiotensins, adrenocorticotropic hormone, or arginine vasopressin were ineffective in displacing the cell-bound radioactivity. Using azidobenzoyl-125I-ANF as a photoaffinity ligand, an ANF receptor with an apparent Mr of 138,000 was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The addition of unlabeled ANF (1 microM) to the incubation medium completely abolished the labeling of this protein band, but atriopeptin I (1 microM) or angiotensins I, II, and III (each 1 microM) were not effective in inhibiting the affinity labeling. The treatment of the neuroblastoma cells with ANF stimulated intracellular cyclic GMP levels in a dose-dependent manner with an EC50 of 5 nM. ANF (1 X 10(-7) M) stimulated cyclic GMP accumulation in less than 5 min by 30-fold as compared to the controls.

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Section: Discussionmentioning

confidence: 88%

entaaification of Atrial Natriuretic Factor Receptor of Neuroblastoma N4TG1 Cells: Binding Characteristics and Photoaffinity Labeling

Pandey

Misono

Takayanagi

et al. 1987

Journal of Neurochemistry

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“…During the preparation of this manuscript, Friedl et al (1985) published evidence that a rat atrial natriuretic peptide (28 amino acids) caused a very large (100to 200-fold) increase in cyclic GMP content in 14-day-old cultures of astroglia-rich brain cells derived from neonatal rat brain. They did not, however, report whether ANF stimulates the release of cyclic GMP from these cells.…”

Section: Discussionmentioning

confidence: 99%

Atrial Natriuretic Factors Stimulate Accumulation and Efflux of Cyclic GMP in C6–2B Rat Glioma and PC12 Rat Pheochromocytoma Cell Cultures

Fiscus

Robles

Waldman

et al. 1987

Journal of Neurochemistry

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Atrial natriuretic factors (ANFs) were tested for their effects on cyclic GMP production in two neurally derived cell lines, the C6-2B rat glioma cells and the PC12 rat pheochromocytoma cells. These cell lines were selected because both are known to possess high amounts of the particulate form of guanylate cyclase, a proposed target of ANF in peripheral organs. Previous studies from our laboratory have shown that ANF selectively activates particulate, but not soluble, guanylate cyclase in homogenates of a variety of rat tissues and that one class of ANF receptor appears to be the same glycoprotein as particulate guanylate cyclase. In the present study we found that four analogs of ANF stimulate cyclic GMP accumulation in both C6-2B and PC12 cells with the rank order of potency being atriopeptin III = atriopeptin II greater than human atrial natriuretic polypeptide greater than atriopeptin I. Atriopeptin II (100 nM) for 20 min elevated cyclic GMP content in C6-2B cells fourfold and in PC12 cells 12-fold. Atriopeptin II (100 nM) for 20 min also stimulated the efflux of cyclic GMP from both C6-2B cells (47-fold) and PC12 cells (12-fold). Accumulation of cyclic GMP in both cells and media was enhanced by preincubation with the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (250 microM). After 20 min of exposure to atriopeptin II, cyclic GMP amounts in the media were equal to or greater than the amounts in the cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Using cul¬ tured rat brain cells, Friedl, Harmening, Schuricht & Hamprecht (1985) and Friedl, Harmening & Hamprecht (1986) have shown that ANP stimulates the formation of intracellular cyclic GMP in primary astrocyte and glial cell lines. In addition, Fiscus, Robles, Waldman & Murad (1987) have demon¬ strated accumulation and efflux of cyclic GMP in the rat C6-2B glioma and PC 12 rat phaeochromocytoma cells.…”

Section: Introductionmentioning

confidence: 98%

“…Incubations were terminated, after removal of the medium, by the addition of 2 ml methanol (Friedl et al 1985). The methanolic extracts were dried under nitrogen, dissolved in acetate buffer (50 mmol/1, pH 6-2; 1 ml) and cyclic GMP was deter¬ mined by radioimmunoassay.…”

Section: Introductionmentioning

confidence: 99%

Specific binding of atrial natriuretic peptide increases cyclic GMP levels in human astrocytoma cells

Lyall

Balmforth²,

Morton³

1988

Journal of Endocrinology

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Specific high-affinity binding sites (dissociation constant 100 pmol/l) for atrial natriuretic peptide (ANP) have been identified in the clone D384 derived from the human astrocytoma cell line G-CCM. Unrelated peptides such as angiotensin II, vasopressin and bradykinin did not compete for these sites. Of the atrial natriuretic peptides studied, both the human and rat ANP competed equally, while peptides with either C- or N-terminal residue missing or with no internal -S-S-bond either competed less effectively or did not compete at all. Human ANP stimulated the cells to increase their intracellular level of cyclic GMP in a time- and dose-dependent manner with maximum stimulation being approached but not reached at concentrations of 1 mumol/l. These results support both the notion that ANP has an important functional role within the brain and the concept of neurotransmitter/neuromodulator communication between neurones and glia.

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Rat atrial natriuretic peptide elevates the level of cyclic GMP in astroglia-rich brain cell cultures

Cited by 38 publications

References 3 publications

entaaification of Atrial Natriuretic Factor Receptor of Neuroblastoma N4TG1 Cells: Binding Characteristics and Photoaffinity Labeling

entaaification of Atrial Natriuretic Factor Receptor of Neuroblastoma N4TG1 Cells: Binding Characteristics and Photoaffinity Labeling

Atrial Natriuretic Factors Stimulate Accumulation and Efflux of Cyclic GMP in C6–2B Rat Glioma and PC12 Rat Pheochromocytoma Cell Cultures

Specific binding of atrial natriuretic peptide increases cyclic GMP levels in human astrocytoma cells

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