RASSF9 promotes NSCLC cell proliferation by activating the MEK/ERK axis

Yuan, Jun; Ju, Qianqian; Zhu, Jie; Jiang, Yun; Yang, Xuechao; Liu, Xiaoyu; Ma, Jinyu; Sun, Cheng; Shi, Jiahai

doi:10.1038/s41420-021-00583-0

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…MAPK-mediated signal transduction is a key factor affecting cell fate processes [ 59 ]. As the core module of the MAPK signal cascade, the MAPK-ERK1/2 signaling pathway is highly conservative [ 60 ]. The extracellular signals are transmitted into the nucleus by ERK1/2 and trigger modifications in the expression of certain proteins in cells [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway

Zheng

Chen

et al. 2023

Animals

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As a member of the neurotrophic family, brain-derived neurotrophic factor (BDNF) provides a key link in the physiological process of mammalian ovarian follicle development, in addition to its functions in the nervous system. The emphasis of this study lay in the impact of BDNF on the proliferation of porcine follicular granulosa cells (GCs) in vitro. BDNF and tyrosine kinase B (TrkB, receptor of BDNF) were detected in porcine follicular GCs. Additionally, cell viability significantly increased during the culture of porcine GCs with BDNF (100 ng/mL) in vitro. However, BDNF knockdown in GCs decreased cell viability and S-phase cells proportion—and BDNF simultaneously regulated the expression of genes linked with cell proliferation (CCND1, p21 and Bcl2) and apoptosis (Bax). Then, the results of the receptor blocking experiment showed that BDNF promoted GC proliferation via TrkB. The high-throughput sequencing showed that BDNF also regulated the expression profiles of miRNAs in GCs. The differential expression profiles were obtained by miRNA sequencing after BDNF (100 ng/mL) treatment with GCs. The sequencing results showed that, after BDNF treatment, 72 significant differentially-expressed miRNAs were detected—5 of which were related to cell process and proliferation signaling pathways confirmed by RT-PCR. Furthermore, studies showed that BDNF promoted GCs’ proliferation by increasing the expression of CCND1, downregulating miR-127 and activating the ERK1/2 signal pathway. Moreover, BDNF indirectly activated the ERK1/2 signal pathway by downregulating miR-127. In conclusion, BDNF promoted porcine GC proliferation by increasing CCND1 expression, downregulating miR-127 and stimulating the MAPK-ERK1/2 signaling cascade.

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Section: Discussionmentioning

confidence: 99%

The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway

Zheng

Chen

et al. 2023

Animals

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“…Elucidating the complex pathogenesis of lung cancer is of great significance for drug development and improvement of the survival rate, which is a promising study area. Aiming at gene alterations, including epidermal growth factor receptor mutation, Kirsten rat sarcoma viral homolog mutation ( 7 , 38 ), epigenetic regulation ( 39 , 40 ) and tumor microenvironment regulation ( 41 ), as well as uncontrolled signaling pathways such as the MEK/ERK signaling pathway ( 42 ) and PI3K/AKT signaling pathway ( 43 ), numerous drugs have been developed and used for the treatment of lung cancer ( 44 , 45 ). In addition, current research has found that certain novel genes are involved in the occurrence and development of lung cancer; for example, MG53 suppressed tumor progression by modulating G3BP2 activity in NSCLC ( 32 ), which is a potential therapeutic target for lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SERPINA3 suppresses tumor progression by modulating SPOP/NF‑κB in lung cancer

et al. 2023

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The pathogenesis mechanism of lung cancer is very complex, with high incidence and mortality. Serpin family A member 3 (SERPINA3) expression levels were reduced in the sera of patients with lung cancer and may be a candidate diagnostic and prognostic survival biomarker in lung cancer, as previously reported. However, the detailed biological functions of SERPINA3 in the pathogenesis of lung cancer remain unknown. In the present study, it was aimed to explore the effects of SERPINA3 on the occurrence of lung cancer. SERPINA3 expression was assessed using bioinformatics database analysis and experimental detection. Then, the biological effects of SERPINA3 were investigated in a cell culture system and a xenograft model of human lung cancer. The potential regulatory mechanism of SERPINA3 in lung cancer was explored by data-independent acquisition mass spectrometry (DIA-MS) detection and further validated by western blotting (WB). The results indicated that SERPINA3 expression levels were significantly downregulated in lung cancer tissues and cell lines. At the cellular level, it was revealed that overexpressed SERPINA3 inhibited cell growth, proliferation, migration and invasion and promoted the apoptosis of lung cancer cells. Moreover, overexpressed SERPINA3 enhanced the sensitivity of lung cancer cells to osimertinib. In vivo , a xenograft model of human lung cancer was established with BALB/c nude mice. After the injection of A549 cells, the tumor growth of the tumor-bearing mice in the SERPINA3-overexpressing group increased more slowly, and the tumor volume was smaller than that in the empty-vector group. Mechanistically, a total of 65 differentially expressed proteins were identified. It was found that the speckle-type POZ protein (SPOP) was significantly upregulated in SERPINA3-overexpressing H157 cells using DIA-MS detection and analysis. WB validation showed that SPOP expression increased, and NF-kappaB (NF-κB) p65 was inhibited in cell lines and tumor tissues of mice when SERPINA3 was overexpressed. The present findings suggest that SERPINA3 is involved in the development of lung cancer and has an antineoplastic role in lung cancer.

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“…Except for KITLG and TMTC3 , the rest of the seven known genes do not appear to be functionally related to chick feather colors ( Table 3 ) [ 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. TSPAN19 was associated with plasma inhibin B levels [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…ALX1 affected craniofacial development and was also closely related to beak shape in Darwin’s finches [ 48 ]. RASSF9 plays a role in regulating tumor proliferation and maintaining epidermal homeostasis [ 49 , 50 , 51 ]. NTS is a neuropeptide that is involved in the regulation of the central nervous system and digestive system and promotes tumor metastasis, etc.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study Identifies Candidate Genes for Stripe Pattern Feather Color of Rhode Island Red Chicks

Shen

Zhou

et al. 2022

Genes

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Feather colors of chickens are not only characteristics of breeds but also as phenotypic markers in chicken breeding. Pure-bred Rhode Island Red (RIR) chicks have a stripe pattern and a non-stripe pattern on the back. The stripe pattern of RIR is generally shown as four longitudinal black stripes on the back and is more likely to appear in females. In this study, we performed a genome-wide association study (GWAS) to identify candidate genes controlling the stripe pattern of RIR chicks, and then, based on physical location and biological functions, quantitative RT-PCR analysis was used to validate the differential expression of candidate genes between stripe pattern and non-stripe pattern back skin tissue. The GWAS showed that a major signal contains 768 significant single nucleotide polymorphisms (SNPs) and 87 significant small insertions-deletions (INDELs) spanning 41.78 to 43.05 Mb (~1.27 Mb) on GGA1, corresponding to 16 genes associated with stripe pattern phenotype. Among these 16 genes, KITLG and TMTC3 could be considered candidate genes as they showed different expressions between back skin tissues of stripe pattern and non-stripe pattern chicks in value (p = 0.062) and the significant level (p < 0.05), respectively. This study provided novel insight into the mechanisms underlying feather pigmentation and stripe formation in RIR chicks.

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RASSF9 promotes NSCLC cell proliferation by activating the MEK/ERK axis

Cited by 5 publications

References 39 publications

The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway

The Mechanisms of BDNF Promoting the Proliferation of Porcine Follicular Granulosa Cells: Role of miR-127 and Involvement of the MAPK-ERK1/2 Pathway

Overexpression of SERPINA3 suppresses tumor progression by modulating SPOP/NF‑κB in lung cancer

Genome-Wide Association Study Identifies Candidate Genes for Stripe Pattern Feather Color of Rhode Island Red Chicks

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