RASSF8-mediated transport of Echinoid via the exocyst promotes<i>Drosophila</i>wing elongation and epithelial ordering

Chan, Eunice HoYee; Zhou, Yanxiang; Aerne, Birgit L.; Holder, Maxine; Weston, Anne; Barry, David J.; Collinson, Lucy; Tapon, Nicolas

doi:10.1242/dev.199731

Cited by 2 publications

(1 citation statement)

References 101 publications

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“…In cluster 2, RASSF8 is provital for embryonic development in Drosophila , where it is involved in pupal wing cell hexagonal packing promoting wing elongation and epithelial ordering ( Chan et al, 2021 ). RASSF8 plays a role in cell growth, regulation of the Wnt and NFκB pathways, and cell-cell adhesion ( Lock et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Gao

Zhang

et al. 2021

Front. Genet.

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Granulosa cells (GCs) are decisive players in follicular development. In this study, the follicle tissues and GCs were isolated from the goose during the peak-laying period to perform hematoxylin-eosin staining and RNA-seq, respectively. Moreover, the dynamic mRNA and lncRNA expression profiles and mRNA-lncRNA network analysis were integrated to identify the important genes and lncRNAs. The morphological analysis showed that the size of the GCs did not significantly change, but the thickness of the granulosa layer cells differed significantly across the developmental stages. Subsequently, 14,286 mRNAs, 3,956 lncRNAs, and 1,329 TUCPs (transcripts with unknown coding potential) were detected in the GCs. We identified 37 common DEGs in the pre-hierarchical and hierarchical follicle stages, respectively, which might be critical for follicle development. Moreover, 3,089 significant time-course DEGs (Differentially expressed genes) and 13 core genes in 4 clusters were screened during goose GCs development. Finally, the network lncRNA G8399 with CADH5 and KLF2, and lncRNA G8399 with LARP6 and EOMES were found to be important for follicular development in GCs. Thus, the results would provide a rich resource for elucidating the reproductive biology of geese and accelerate the improvement of the egg-laying performance of geese.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Gao

Zhang

et al. 2021

Front. Genet.

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The cell adhesion molecule Echinoid promotes tissue survival and separately restricts tissue overgrowth inDrosophilaimaginal discs

Spitzer

Sun

Rodríguez-Vargas

et al. 2023

Preprint

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The interactions that cells inDrosophilaimaginal discs have with their neighbors are known to regulate their ability to survive. In a screen of genes encoding cell surface proteins for gene knockdowns that affect the size or shape of mutant clones, we found that clones of cells with reduced levels ofechinoid(ed) are fewer, smaller, and can be eliminated during development. In contrast, discs composed mostly ofedmutant tissue are overgrown. We find thatedmutant tissue has lower levels of the anti-apoptotic protein Diap1 and has increased levels of apoptosis which is consistent with the observed underrepresentation ofedmutant clones and the slow growth ofedmutant tissue. The eventual overgrowth ofedmutant tissue results not from accelerated growth, but from prolonged growth resulting from a failure to arrest growth at the appropriate final size. Ed has previously been shown to physically interact with multiple Hippo-pathway components and it has been proposed to promote Hippo pathway signaling, to exclude Yorkie (Yki) from the nucleus, and restrain the expression of Yki-target genes. We did not observe changes in Yki localization inedmutant tissue and found decreased levels of expression of several Yorkie-target genes, findings inconsistent with the proposed effect of Ed on Yki. We did, however, observe increased expression of several Yki-target genes in wild-type cells neighboringedmutant cells, which may contribute to elimination ofedmutant clones. Thus,edhas two distinct functions: an anti-apoptotic function by maintaining Diap1 levels, and a function to arrest growth at the appropriate final size. Both of these are unlikely to be explained by a simple effect on the Hippo pathway.

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RASSF8-mediated transport of Echinoid via the exocyst promotesDrosophilawing elongation and epithelial ordering

Cited by 2 publications

References 101 publications

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

Genome-Wide Gene Expression Profiles Reveal Distinct Molecular Characteristics of the Goose Granulosa Cells

The cell adhesion molecule Echinoid promotes tissue survival and separately restricts tissue overgrowth inDrosophilaimaginal discs

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