Raspberry Ketone Promotes the Differentiation of C3H10T1/2 Stem Cells into Osteoblasts

Takata, Tsutomu; Morimoto, Chie

doi:10.1089/jmf.2013.2763

Cited by 14 publications

(5 citation statements)

References 42 publications

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“…The multipotent stem cell line C3H10T1/2, which has the potential to be differentiated into adipocytes, chondrocytes, osteocytes or myocytes [24], is a widely used model to study the modulations of stem cell fate under nutritional and environmental influence [25–29]. Consistent with the effects of parabens on 3T3-L1 adipocyte differentiation [14], butylparaben markedly promoted adipogenic differentiation (Fig.…”

Section: Discussionmentioning

confidence: 93%

Methylparaben and butylparaben alter multipotent mesenchymal stem cell fates towards adipocyte lineage

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Heal

et al. 2017

Toxicology and Applied Pharmacology

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Paraben esters and their salts are widely used as preservatives in cosmetics, personal care products, pharmaceuticals, and foods. We previously reported that parabens promoted adipocyte differentiation in vitro and increased adiposity but suppressed serum marker of bone formation in vivo. Here, we investigated the effects of parabens (methylparaben and butylparaben) on modulating cell fate of multipotent stem cell line C3H10T1/2. Both parabens modulated adipogenic, osteogenic, and chondrogenic differentiation of C3H10T1/2 cells in vitro. Butylparaben markedly promoted adipogenic differentiation, but suppressed osteogenic and chondrogenic differentiation whereas methylparaben showed similar but less pronounced effects. Moreover, butylparaben, but not methylparaben, was shown to activate peroxisome proliferator-activated receptor (PPAR) γ whereas neither of the paraben was shown to activate glucocorticoid receptor (GR) responsive reporter in C3H10T1/2 cells. The adipogenic effects of butylparaben were significantly attenuated by PPARγ knockdown, but not by GR knockdown. In contrast, paraben’s effects on osteoblast differentiation were affected by both knockdowns. Collectively, the results demonstrate opposing effects of parabens on adipogenic and osteoblastogenic/chondrogenic differentiation of multipotent stem cells. In light of the recent findings that parabens are detected in human placenta and milk, our studies provide rationales to study paraben exposure during early development of life in the future.

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Section: Discussionmentioning

confidence: 93%

Methylparaben and butylparaben alter multipotent mesenchymal stem cell fates towards adipocyte lineage

Overby

Heal

et al. 2017

Toxicology and Applied Pharmacology

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“…This cell line was chosen for its maintained capacity to differentiate into different tissues such as bone, cartilage, adipose tissue, etc. , Shea demonstrated that BMP2 treatment of C3H10T1/2 mesenchymal stem cells induces both chondrogenesis and osteogenesis. Takata used them to evaluate the potential of a specific ketone found in raspberries to promote osteogenesis. In the present study, it should be specified that the C3H10T1/2 were seeded on the scaffold in the absence of any differentiation factor to avoid any guided differentiation toward bone lineage that could be only to the result of the presence of BMP2.…”

Section: Discussionmentioning

confidence: 99%

Poly(ε-caprolactone)/Hydroxyapatite 3D Honeycomb Scaffolds for a Cellular Microenvironment Adapted to Maxillofacial Bone Reconstruction

Garcia

Hébraud

Duval

et al. 2018

ACS Biomater. Sci. Eng.

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The elaboration of biomimetic materials inspired from the specific structure of native bone is one the main goal of tissue engineering approaches. To offer the most appropriate environment for bone reconstruction, we combined electrospinning and electrospraying to elaborate an innovative scaffold composed of alternating layers of polycaprolactone (PCL) and hydroxyapatite (HA). In our approach, the electrospun PCL was shaped into a honeycomb-like structure with an inner diameter of 160 μm, capable of providing bone cells with a 3D environment while ensuring the material biomechanical strength. After 5 days of culture without any differentiation factor, the murine embryonic cell line demonstrated excellent cell viability on contact with the PCL-HA structures as well as active colonization of the scaffold. The cell differentiation, as tested by RT-qPCR, revealed a 6-fold increase in the expression of the RNA of the Bglap involved in bone mineralization as compared to a classical 2D culture. This differentiation of the cells into osteoblasts was confirmed by alkaline phosphatase staining of the scaffold cultivated with the cell lineage. Later on, organotypic cultures of embryonic bone tissues showed the high capacity of the PCL-HA honeycomb structure to guide the migration of differentiated bone cells throughout the cavities and the ridge of the biomaterial, with a colonization surface twice as big as that of the control. Taken together, our results indicate that PCL-HA honeycomb structures are biomimetic supports that promotes in vitro osteocompatibility, osteoconduction, and osteoinduction and could be suitable for being used for bone reconstruction in complex situations such as the repair of maxillofacial defects.

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“…Raspberry volatiles are important for the perception of sensory quality (odor, flavor) and for mold resistance [ 13 ], and some are claimed to have nutraceutical properties [ 28 , 29 ], although the nutritional role of volatile compounds is still very controversial. Fruit volatile compounds are influenced by numerous factors including cultivar variation, climate, soil, ripeness, and many other variables [ 11 , 19 , 30 ].…”

Section: Volatile Compounds In Raspberrymentioning

confidence: 99%

Volatile Compounds of Raspberry Fruit: From Analytical Methods to Biological Role and Sensory Impact

2015

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Volatile compounds play a key role in the formation of the well-recognized and widely appreciated raspberry aroma. Studies on the isolation and identification of volatile compounds in raspberry fruit (Rubus idaeus L.) are reviewed with a focus on aroma-related compounds. A table is drawn up containing a comprehensive list of the volatile compounds identified so far in raspberry along with main references and quantitative data where available. Two additional tables report the glycosidic bond and enantiomeric distributions of the volatile compounds investigated up to now in raspberry fruit. Studies on the development and evolution of volatile compounds during fruit formation, ripening and senescence, and genetic and environmental influences are also reviewed. Recent investigations showing the potential role of raspberry volatile compounds in cultivar differentiation and fruit resistance to mold disease are reported as well. Finally a summary of research done so far and our vision for future research lines are reported.

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Raspberry Ketone Promotes the Differentiation of C3H10T1/2 Stem Cells into Osteoblasts

Cited by 14 publications

References 42 publications

Methylparaben and butylparaben alter multipotent mesenchymal stem cell fates towards adipocyte lineage

Methylparaben and butylparaben alter multipotent mesenchymal stem cell fates towards adipocyte lineage

Poly(ε-caprolactone)/Hydroxyapatite 3D Honeycomb Scaffolds for a Cellular Microenvironment Adapted to Maxillofacial Bone Reconstruction

Volatile Compounds of Raspberry Fruit: From Analytical Methods to Biological Role and Sensory Impact

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