Rasayans and non-rasayans herbs: Future immunodrug - Targets

Doshi, Gaurav Mahesh; Une, Hemant Devidas; Shanbhag, Pradnya Palekar

doi:10.4103/0973-7847.120506

Cited by 30 publications

(16 citation statements)

References 22 publications

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“…Vetiver grass is also grown for the production of oil which is used in perfumery and aromatherapy [ 15 ]. Imperata cylindrica L., commonly known as thatch grass or cogon grass, possess immunomodulatory, neuroprotective, anticancer, and antioxidant activities [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan

Fatima

Kanwal

Mahmood

2018

BMC Complement Altern Med

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BackgroundOxidative stress as well as bacterial and fungal infections are common source of diseases while plants are source of medication for curative or protective purposes. Hence, aim of study was to compare the pharmacological potential of seven grass species in two different solvents i.e. ethanol and acetone.MethodsPreliminary phytochemical tests were done and antioxidant activities were evaluated using ELISA and their IC50 values and AAI (%) were recorded. ANOVA was used for statistical analyses. DNA damage protection assay was done using p1391Z plasmid DNA and DNA bands were analyzed. Antimicrobial activity was done via disc diffusion method and MIC and Activity Index were determined. Cytotoxic activity was carried out using the brine shrimps’ assay and LC50 values were calculated using probit analysis program.ResultsPhytochemical studies confirmed the presence of secondary metabolites in most of the plant extracts. Maximum antioxidant potential was revealed in DiAEE, DiAAE (AAI- 54.54% and 43.24%) and DaAEE and DaAAE (AAI- 49.13% and 44.52%). However, PoAEE and PoAAE showed minimum antioxidant potential (AAI- 41.04% and 34.11%). SaSEE, DiAEE and ElIEE showed very little DNA damage protection activity. In antimicrobial assay, DaAEE significantly inhibited the growth of most of the microbial pathogens (nine microbes out of eleven tested microbes) among ethanol extracts while DaAAE and ImCAE showed maximum inhibition (eight microbes out of eleven tested microbes) among acetone plant extracts. However, PoAEE and PoAAE showed least antimicrobial activity. F. oxysporum and A. niger were revealed as the most resistant micro-organisms. ImCEA and ImCAE showed maximum cytotoxic potential (LC50 11.004 ppm and 7.932 ppm) as compared to the other plant extracts.ConclusionFodder grasses also contains a substantial phenols and flavonoids contents along with other secondary metabolites and, hence, possess a significant medicinal value. Ethanol extracts showed more therapeutic potential as compared to the acetone extracts. This study provides experimental evidence that the selected species contains such valuable natural compounds which can be used as medicinal drugs in future.

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Section: Introductionmentioning

confidence: 99%

Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan

Fatima

Kanwal

Mahmood

2018

BMC Complement Altern Med

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“…[3][4][5] Medicinal and local plants researches in the field of immunology have brought an immense breakthrough by influencing the system as well as playing their role as an immunoadjuvants, immunosuppressant and immunostimulants. 6 India's cultural heritage system has put its roots in supporting Glycyrrhiza glabra, Uncaria tomentosa, Tinospora cordifolia, Allium asativum, Aloe vera, Angrographis paniculata, Asparagus racemosus, Azadirachta indica, Curcuma longa, Nycanthes arbortristis, Ocimmum sanctum, Panax ginseng, Pylanthus embilica, Withania somnifera, Hibiscus esculentum, Mangifera indica, Piper betel and Saraca indica with immunomodulatory perspectives etc.. 2,7 Albizia lebbeck (AL, commonly known as Shirish) has been comprehensively recommended to remove toxins from the body. The plant is a deciduous woody tree, approx.12-21 m in height, having pale bark with glabrous young shoots.…”

Section: Introductionmentioning

confidence: 99%

Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts

Doshi¹,

Pawar²,

Chavda³

2016

Phcogj

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Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 ± 102.78, 597.33 ± 85.35, 426.66 ± 53.98) and DTH Delayed-Type Hypersensitivity reaction (0.225±0.01, 0.21 ± 0.01, 0.23 ± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5×10 9) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 μg/ml, 20 μg/ml, 40 μg/ml, 80 μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

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“…The classical medicines reported from the selected plant are Kushmanda avaleha, Vasakhanda, Khanda and Rasayana. [2][3][4] Due to its rasayana property, it is beneficial in improving immune protection and is advised during the degenerative phase of life around 45 years in both the sexes. [5] The fruit contains good amount of proteins, enzymes, vitamin B 1 and C, flavonoid C-glycoside, terpenes, phenolic acids and free sugars such as glucose, rhamnose, mannitol, uronic acid and some trace metals which are beneficial in treatment of various diseases such as diabetes, cancer, inflammatory disorders, convulsions and infections.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic studies on Benincasa hispida (thunb.) Cogn. Seed extract scrutinized by HPLC and HPTLC

Une¹,

Doshi²

2014

Phcog J.

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Background: Benincasa hispida (Thunb.) Cogn. is an extensive climbing annual herb in an agricultural country like India. Lupeol, a constituent of this species, has been reported to possess good amount of pharmacological potential. Objective: In the current studies, the research team focused on determining the percentage of the lupeol present in the extract of Benincasa hispida seeds by chromatographic techniques. Materials and Methods: Shade-dried seeds of Benincasa hispida were subjected to soxhlet extraction followed by scrutinization of the lupeol contents by HPTLC and HPLC methods after carrying out preliminary phytochemical screening for the constituents present in the extract. Results: The extraction yield was found to be 1.2% (w/w). Phytochemical screening of the extract revealed the presence of carbohydrates, glycosides, alkaloids, fixed oils and fats, tannins phenolic compounds, steroids and flavonoids. The amount of lupeol present in the seeds extract was found to be 0.47% w/w (HPTLC)and 6.85% w/v (HPLC) by HPTLC quantification and HPLC analysis respectively. Research studies showed a peak which coincided with the peak of standard lupeol signifying the presence of lupeol in the extract. Conclusion: The extract contains significant amount of lupeol.

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Rasayans and non-rasayans herbs: Future immunodrug - Targets

Cited by 30 publications

References 22 publications

Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan

Evaluation of biological potential of selected species of family Poaceae from Bahawalpur, Pakistan

Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts

Chromatographic studies on Benincasa hispida (thunb.) Cogn. Seed extract scrutinized by HPLC and HPTLC

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