RAS, FLT3, and C-KIT mutations in immunophenotyped canine leukemias

Canine lymphomas: DNA changes in tumour genes

“…The RAS genes (N, K and H) encode proteins important in cell signal transduction. Although missense mutations in N-RAS gene were detected by Usher et al (2009) in 25 % dogs with acute lymphoid leukaemia, our results indicated that N-RAS gene activation in canine lymphoma is rarely, like as considered Mayr et al (2002).…”

Detection of Mutations in Selected Proto-Oncogenes of Canine Lymphoma

“…3b) in the intron region behind exon 20, in 242 nt position. In the sec- has already been observed in dogs [13] and does not affect the significance of the codon. Our results agree with those of G i a n t i n et al [3] according to which the occurrence of mutations in canine lymphoma C-KIT gene is rare.…”

“…In relation to the damage in the C-KIT gene, cases have been described of acute myeloid leukaemia [13] and adipose cell tumours [6]. L o n d o n et al [6] reported the occurrence of intragene tandem duplications in exons 11 and 12 associated with tumour diseases of mastocytes.…”

“…L o n d o n et al [6] reported the occurrence of intragene tandem duplications in exons 11 and 12 associated with tumour diseases of mastocytes. In dogs with acute myeloid leukaemia diagnosis, U s h e r et al [13] found silent mutations in exon 8, codon 425, and a number of point mutations resulting in changes in AMK sequence in the exon 17. In the aggressive canine T-cell lymphomas, G i a n t i n et al [3] In our study we observed no change in genetic code in comparison with reference sequence, which is in agreement with observations of M a y r et al [7] who reported that activation of proto-oncogene NRAS in canine lymphoma is rare.…”

“…Mutations in proto-oncogenes C-KIT, FLT3 and NRAS have been described in association with a number of haematological malignancies including lymphomas. The frequency of these mutations in canine lymphomas has not been specified [13].…”