Rare sugar d-psicose protects pancreas β-islets and thus improves insulin resistance in OLETF rats

Hossain, Akram; Yamaguchi, Fuminori; Matsunaga, Toru; Hirata, Yuko; Kamitori, Kazuyo; Dong, Yan; Sui, Li; Tsukamoto, Ikuko; Ueno, Masaki; Tokuda, Masaaki

doi:10.1016/j.bbrc.2012.07.135

Cited by 54 publications

(71 citation statements)

References 26 publications

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“…In all our studies, we have observed striking differences in the histological findings pertaining to the morphology of the pancreas islets of Langerhans between D-allulose-fed and control animals (Hossain et al, 2012;Hossain et al, 2015). Briefly, islets of OLETF control rats were observed to be large, disorganized with irregular shapes, and separated into clusters by connective tissue bands, with expansion into adjacent exocrine tissues.…”

Section: Anti-hyperglycemic and Anti-inflammatory Effects Of D-allulomentioning

confidence: 70%

“…weeks (Hossain et al, 2012). To elucidate the islet architecture and -cell loss in a long-term chronic disease study, we performed both hematoxylin and eosin staining and immunofluorescence staining using anti-insulin and anti-glucagon antibodies.…”

Section: Anti-hyperglycemic and Anti-inflammatory Effects Of D-allulomentioning

confidence: 99%

“…Therefore, it is of prime importance to look for effective therapeutic interventions for both the prevention and treatment of diabetes and its complications (Takeuchi et al, 1996). The rare sugar D-allulose has attracted much attention for its promising anti-hyperglycemic and anti-hyperlipidemic effects in experimental studies (Matsuo et al, 1999;Matsuo and Izumori, 2006;Matsuo and Izumori, 2009;Hossain et al, 2011;Hossain et al, 2012;Hossain et al, 2015).…”

Section: Clinical Trials Of D-allulose On Normal and Borderline Diabementioning

confidence: 99%

“…2006; Yagi and Matsuo, 2009;Hossain et al, 2011;Chung et al, 2012;Hossain et al, 2012;Ochiai et al, 2013;Ochiai et al, 2014) and humans . Because obesity is known to cause insulin resistance, leading to T2DM, D-allulose could be useful in maintaining a healthy body weight and preventing the onset of diabetes.…”

mentioning

confidence: 99%

“…In another study, 5% D-allulose in the diet lowered the overall carcass fat content, and specifically reduced the abdominal adipose tissue weight with no difference in body weight gain in comparison to sucrose and D-fructose (Matsuo and Izumori, 2006). In T2DM model Collectively, D-allulose appears to have anti-obesity potential, which is perhaps due to diminished food intake (Hossain et al, 2011;Chung et al, 2012;Hossain et al, 2012;Ochiai et al, 2013;Ochiai et al, 2014). Diminished body weight and lowered carcass fat have also been observed when rats were pair-fed high-sucrose diets with or without D-allulose (Ochiai et al, 2014).…”

mentioning

confidence: 99%

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Rare sugar d-allulose: Potential role and therapeutic monitoring in maintaining obesity and type 2 diabetes mellitus

Hossain

Yamaguchi

Matsuo

et al. 2015

Pharmacology & Therapeutics

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117

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Section: Anti-hyperglycemic and Anti-inflammatory Effects Of D-allulomentioning

confidence: 70%

Section: Anti-hyperglycemic and Anti-inflammatory Effects Of D-allulomentioning

confidence: 99%

Section: Clinical Trials Of D-allulose On Normal and Borderline Diabementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Rare sugar d-allulose: Potential role and therapeutic monitoring in maintaining obesity and type 2 diabetes mellitus

Hossain

Yamaguchi

Matsuo

et al. 2015

Pharmacology & Therapeutics

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158

117

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Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp.

et al. 2021

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D-Allulose has potential as a low-calorie sweetener which can suppress fat accumulation. Several enzymes capable of D-allulose production have been isolated, including D-tagatose 3-epimerases. Here, we report the isolation of a novel protein from Methylomonas sp. expected to be a putative enzyme based on sequence similarity to ketose 3-epimerase. The synthesized gene encoding the deduced ketose 3-epimerase was expressed as a recombinant enzyme in Escherichia coli, and it exhibited the highest enzymatic activity toward L-ribulose, followed by D-ribulose and D-allulose. The X-ray structure analysis of L-ribulose 3-epimerase from Methylomonas sp. (MetLRE) revealed a homodimeric enzyme, the first reported structure of dimeric Lribulose 3-epimerase. The monomeric structure of MetLRE is similar to that of homotetrameric L-ribulose 3-epimerases, but the short C-terminal a-helix of MetLRE is unique and different from those of known L-ribulose 3 epimerases. The length of the C-terminal a-helix was thought to be involved in tetramerization and increasing stability; however, the addition of residues to MetLRE at the C terminus did not lead to tetramer formation. MetLRE is the first dimeric L-ribulose 3-epimerase identified to exhibit high relative activity toward D-allulose. D-Allulose (alternative name D-psicose) is a rare sugar, and its physiological functions, such as altering the blood glucose level, suppressing fat accumulation, and use as a low-calorie sweetener [1-9], have been focused on as a promising functional food ingredient. In Japan, a low-calorie syrup containing D-allulose, Rare Sugar Sweet Ò (Matsutani Chemical Industry Co., Ltd., Hyogo, Japan) [10], is commercially available and its labeling as a functional food was recently approved. In addition, the U.S. Food and Drug Administration (FDA) stated that 'it allows the low-calorie sweetener allulose to be excluded from total and added sugar counts on Nutrition and Supplement Facts labels when used as an ingredient' (FDA In Brief, April, 2019). AbbreviationsAgDAE, D-allulose 3-epimerase from Arthrobacter globiformis; AtDAE, D-allulose 3-epimerase from Agrobacterium tumefaciens; CcDAE, D-allulose 3-epimerase from Clostridium cellulolyticum; DAE, D-allulose 3-epimerase; his_MetLRE long2, his_MetLRE with nine additional residues (TAIKTIELH) at the C terminus and with three mutations (Y9I, Y37F, Y286L); his_MetLRE, N-terminal his-tagged MetLRE; his_MetLRE3, his_MetLRE with three mutations (Y9I, Y37F, and Y286L); LRE, L-ribulose 3-epimerase; MetLRE, L-ribulose 3-epimerase from Methylomonas sp.; MlLRE, L-ribulose 3-epimerase from Mesorhizobium loti; PcDTE, D-tagatose 3-epimerases from Pseudomonas cichorii.

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Biosynthesis of rare ketoses through constructing a recombination pathway in an engineered Corynebacterium glutamicum

Yang

Zhu

et al. 2014

Biotech & Bioengineering

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Rare sugars have various known biological functions and potential for applications in pharmaceutical, cosmetics, and food industries. Here we designed and constructed a recombination pathway in Corynebacterium glutamicum, in which dihydroxyacetone phosphate (DHAP), an intermediate of the glycolytic pathway, and a variety of aldehydes were condensed to synthesize rare ketoses sequentially by rhamnulose-1-phosphate aldolase (RhaD) and fructose-1-phosphatase (YqaB) obtained from Escherichia coli. A wild-type strain harboring this artificial pathway had the ability to produce D-sorbose and D-psicose using D-glyceraldehyde and glucose as the substrates. The tpi gene, encoding triose phosphate isomerase was further deleted, and the concentration of DHAP increased to nearly 20-fold relative to that of the wild-type. After additional optimization of expression levels from rhaD and yqaB genes and of the fermentation conditions, the engineered strain SY6(pVRTY) exhibited preferable performance for rare ketoses production. Its yield increased to 0.59 mol/mol D-glyceraldehyde from 0.33 mol/mol D-glyceraldehyde and productivity to 2.35 g/L h from 0.58 g/L h. Moreover, this strain accumulated 19.5 g/L of D-sorbose and 13.4 g/L of D-psicose using a fed-batch culture mode under the optimal conditions. In addition, it was verified that the strain SY6(pVRTY) meanwhile had the ability to synthesize C4, C5, C6, and C7 rare ketoses when a range of representative achiral and homochiral aldehydes were applied as the substrates. Therefore, the platform strain exhibited the potential for microbial production of rare ketoses and deoxysugars.

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Rare sugar d-psicose protects pancreas β-islets and thus improves insulin resistance in OLETF rats

Cited by 54 publications

References 26 publications

Rare sugar d-allulose: Potential role and therapeutic monitoring in maintaining obesity and type 2 diabetes mellitus

Rare sugar d-allulose: Potential role and therapeutic monitoring in maintaining obesity and type 2 diabetes mellitus

Crystal structure of a novel homodimeric l‐ribulose 3‐epimerase from Methylomonus sp.

Biosynthesis of rare ketoses through constructing a recombination pathway in an engineered Corynebacterium glutamicum

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