Rare carotenoids, (3R)-saproxanthin and (3R,2′S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidative activities

Abstract

We isolated three orange or yellow pigment-producing marine bacteria, strains 04OKA-13-27 (MBIC08261), 04OKA-17-12 (MBIC08260), and YM6-073 (MBIC06409), off the coast of Okinawa Prefecture in Japan. These strains were classified as novel species of the family Flavobacteriaceae based on their 16S rRNA gene sequence. They were cultured, and the major carotenoids produced were purified by chromatographic methods. Their structures were determined by spectral data to be (3R)-saproxanthin (strain 04OKA-13-27), (3R,2'S)-myxol (strain YM6-073), and (3R,3'R)-zeaxanthin (strains YM6-073 and 04OKA-17-12). Saproxanthin and myxol, which are monocyclic carotenoids rarely found in nature, demonstrated significant antioxidative activities against lipid peroxidation in the rat brain homogenate model and a neuro-protective effect from L-glutamate toxicity.

Hydroxy-3,4-dehydro-apo-8′-lycopene and methyl hydroxy-3,4-dehydro-apo-8′-lycopenoate, novel C30 carotenoids produced by a mutant of marine bacterium Halobacillus halophilus

“…[7][8][9] These studies have reported the isolation of rare carotenoids, (3R)-saproxanthin and myxol, from Flavobacteriaceae, 7 and novel glyco-C 30 carotenoids (diapolycopenedioic acid xylosyl esters A, B, and C from Rubritalea squalenifaciens 8 and methyl glucosyl-3,4-dehydroapo-8¢-lycopenoate from Planococcus maritimus 9 ).…”

Elevated seawater temperature causes a microbial shift on crustose coralline algae with implications for the recruitment of coral larvae

“…Similarly, diseased corals and sponges host a much greater abundance of Bacteroidetes than their healthy counterparts (Frias-Lopez et al, 2002;Pantos and Bythell, 2006;Webster et al, 2008b). The closest relative to the Bacteroidetes sequence that became highly abundant at 32 1C was a novel marine Flavobacteria with anti-oxidative properties (Shindo et al, 2007). The Bacteroidetes are diverse and abundant in the marine ecosystem (O'Sullivan et al, 2006) and are major utilizers of high-molecular mass dissolved organic matter (Cottrell and Kirchman, 2000).…”

Diapolycopenedioic Acid Xylosyl Esters A, B, and C, Novel Antioxidative Glyco-C30-carotenoic Acids Produced by a New Marine Bacterium Rubritalea squalenifaciens

“…Recently, we have initiated the analysis of novel or rare types of carotenoids from a new colored marine bacterium, which was classified as belonging to a new species by 16S rRNA analyses [7]. Rubritalea squalenifaciens (strain HOact23 T ; MBIC08254 T ) is a rare marine bacterium belonging to subdivision 1 of Verrucomicrobia, a Gramnegative, heterotrophic mesophile, which was isolated from the marine sponge Halichondria okdai.…”