Rare actinobacteria: a potential source of bioactive polyketides and peptides

Bundale, Sunita; Singh, Jaya; Begde, Deovrat; Nashikkar, Nandita; Upadhyay, Avinash

doi:10.1007/s11274-019-2668-z

Cited by 23 publications

(13 citation statements)

References 29 publications

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“…One of the main strategies in the search for new sources of bioactive compounds is the isolation of non-streptomy cete actinobacteria; also known as rare actinobacteria which are often difficult to isolate and cultivate; from underex plored and uncommon habitats (Baltz 2017;Bundale et al 2019;Goodfellow et al 2018). In this context, the Sahara is a good example of the extreme habitats that is unfavorable for life, apart from microbial life that has become adapted to the harsh conditions.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Meklat

Bouras

Mokrane

et al. 2020

Geomicrobiology Journal

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The Sahara, one of the most extreme environments on Earth, constitutes an unexplored source of alkalitolerant actinobacteria. ln this worl<, we studied the diversity of alkalitolerant actinobacteria in various soils collected from different regions of the Algerian Sahara. A total of 29 alkalitolerant actinobacterial strains were isolated by using a complex agar medium. The diversity of these acti nobacteria was evaluated using a polyphasic approach, which included morphological, chemotaxo nomic, physiological (numerical taxonomy) and 16S rRNA gene analyses. The isolates which were assigned to the genus Nocardiopsis, shared relatively low 16S rRNA gene sequences similarities compared to closely related species suggesting that they belonged to putatively new species. Ali of the strains were tested for antibiotic activity against a broad range of microorganisms and screened for genes encoding polyketide synthases and non-ribosomal peptide synthetases and found to have the potential to produce secondary metabolites. Consequently, the study supports the view that extrerne environments contain many nove! actinobacteria, which represent an unex plored source for the discovery of biologically active compounds.

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Section: Introductionmentioning

confidence: 99%

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Meklat

Bouras

Mokrane

et al. 2020

Geomicrobiology Journal

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“…In contrast to other extensively studied actinobacteria, such as Streptomyces , a large number of minor genera and species that could have the potential to be the source of novel chemical entities of pharmaceutical interest have not yet been cultivated under laboratory conditions [ 3 ]. The Kribbella genus belongs to this group of less exploited actinobacteria, also referred to as “rare actinobacteria” [ 16 ]. This genus, which was originally identified as part of the genus Nocardioides in 1989 and reclassified in 1999 as a new genus [ 17 , 18 ], constitutes a potential producer of some new active antimicrobials.…”

Section: Resultsmentioning

confidence: 99%

Kribbellichelins A and B, Two New Antibiotics from Kribbella sp. CA-293567 with Activity against Several Human Pathogens

et al. 2022

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Current needs in finding new antibiotics against emerging multidrug-resistant superbugs are pushing the scientific community into coming back to Nature for the discovery of novel active structures. Recently, a survey of halophilic actinomyectes from saline substrates of El Saladar del Margen, in the Cúllar-Baza depression (Granada, Spain), led us to the isolation and identification of 108 strains from the rhizosphere of the endemic plant Limonium majus. Evaluation of the potential of these strains to produce new anti-infective agents against superbug pathogens was performed through fermentation in 10 different culture media using an OSMAC approach and assessment of the antibacterial and antifungal properties of their acetone extracts. The study allowed the isolation of two novel antibiotic compounds, kribbellichelin A (1) and B (2), along with the known metabolites sandramycin (3), coproporphyrin III (4), and kribelloside C (5) from a bioassay-guided fractionation of scaled-up active extracts of the Kribbella sp. CA-293567 strain. The structures of the new molecules were elucidated by ESI-qTOF-MS/MS, 1D and 2D NMR, and Marfey’s analysis for the determination of the absolute configuration of their amino acid residues. Compounds 1–3 and 5 were assayed against a panel of relevant antibiotic-resistant pathogenic strains and evaluated for cytotoxicity versus the human hepatoma cell line HepG2 (ATCC HB-8065). Kribbellichelins A (1) and B (2) showed antimicrobial activity versus Candida albicans ATCC-64124, weak potency against Acinetobacter baumannii MB-5973 and Pseudomonas aeruginosa MB-5919, and an atypical dose-dependent concentration profile against Aspergillus fumigatus ATCC-46645. Sandramycin (3) confirmed previously reported excellent growth inhibition activity against MRSA MB-5393 but also presented clear antifungal activity against C. albicans ATCC-64124 and A. fumigatus ATCC-46645 associated with lower cytotoxicity observed in HepG2, whereas Kribelloside C (5) displayed high antifungal activity only against A. fumigatus ATCC-46645. Herein, we describe the processes followed for the isolation, structure elucidation, and potency evaluation of these two new active compounds against a panel of human pathogens as well as, for the first time, the characterization of the antifungal activities of sandramycin (3).

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“…In recent years, it has been reported that Streptomyces strains isolated from rhizosphere soil of chickpea, Humulus lupulus and olive plants, which had multiple growth-promoting properties of producing IAA, secreting siderophores, dissolving phosphate, fixing nitrogen, and inhibiting pathogens (Sreevidya et al 2016;Özdemir et al 2019;Dede et al 2020), could promote the growth of tomatoes, eucalyptus and wheat were isolated from leaf, stem, and root samples of 15 tea cultivars, the dominant bacteria was Streptomyces, and IAA production and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activities were recorded in 93.5% and 21.7% of all isolates respectively, the results indicated that endophytic actinomycetes from tea plants also had the potential to promote plant growth (Shan et al 2018). However, rare actinobacteria containing growth-promoting properties has seldom been reported (Bundale et al 2019). Tsukamurella sp.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Han

Zhang

2021

Preprint

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Phosphate Solubilizing Microorganisms widely exist in plant rhizosphere soil, but report about the P solubilization and multiple growth-promoting properties of rare actinomycetes are scarce. In this paper, a phosphate solubilizing Tsukamurella tyrosinosolvens P9 strain was isolated from the rhizosphere soil of tea plants. Phosphorus-dissolving abilities of this strain were different under different carbon and nitrogen sources, the soluble phosphorus content was 442.41 mg/L with glucose and potassium nitrate as nutrient sources. The secretion of various organic acids, such as lactic acid, maleic acid, oxalic acid, etc, was the main mechanism for P solubilization and pH value in culture was very significant negative correlation with soluble P content. In addition, this strain had multiple growth-promoting characteristics with 37.26 μg/mL of IAA and 72.01% of siderophore relative content. Under pot experiments, P9 strain improved obviously the growth of peanut seedlings. The bacterial communities of peanut rhizoshpere soil were assessed after inoculated with P9 strain. It showed that there was no significant difference in alpha-diversity indices between the inoculation and control groups, but the P9 treatment group changed the composition of bacterial communities, which increased the relative abundance of beneficial and functional microbes, which relative abundances of Chitinophagaceae and Beijerinckiaceae at the family level, and of Flavihumibacter , Ramlibacter and Microvirga at the genus level, were all siginificant increased. Specially, Tsukamurella tyrosinosolvens were only detected in the rhizosphere of the inoculated group. This study not only founded growth-promoting properties of T . tyrosinosolvens P9 strain and its possible phosphate solublizing mechanism, but also expected to afford an excellent strain resource in biological fertilizers.

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Rare actinobacteria: a potential source of bioactive polyketides and peptides

Cited by 23 publications

References 29 publications

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Kribbellichelins A and B, Two New Antibiotics from Kribbella sp. CA-293567 with Activity against Several Human Pathogens

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

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Rare actinobacteria: a potential source of bioactive polyketides and peptides

Cited by 23 publications

References 29 publications

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Isolation, Classification and Antagonistic Properties of Alkalitolerant Actinobacteria from Algerian Saharan Soils

Kribbellichelins A and B, Two New Antibiotics from Kribbella sp. CA-293567 with Activity against Several Human Pathogens

Identification&nbsp;of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens&nbsp;Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

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Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting