Rapsų įvairovės tyrimai molekuliniais metodais

Abstract

Oilseed rape (Brassica napus L.) is grown all over the world, and in Lithuania its plots are expanding every year. It is used for oil, feed, fuel, etc. Genetic variability of oilseed rape in Lithuania was analysed employing RAPD molecular markers. Three different random Lithuanian regions in Kėdainiai, Vilkaviškis and Jurbarkas were selected for collecting oilseed rape leaf samples. 'Baldur' (A) leaves were collected in the Kėdainiai region, 'Olano' (O) in the Vilkaviškis region and 'SW Pastell' (P), 'Remy' (R), 'Banjo' (B) in the Jurbarkas region. Nine primers were screened for their ability to produce polymorphic patterns. Eight primers which gave reproducible and distinct amplification products were selected for evaluation of diversity in five oilseed rape cultivars. The test primers generated different polymorphic fragments ranging from 1 to 10 per reaction, with an average of 6.75 bands per primer -in total 54 polymorphic amplification products in the range of 250-1750 bp. Dendrograms based on UPGMA cluster analysis confirmed the suitability of all the primers for the further analysis and showed a significant genetic variation among individuals of different cultivars.

The influence of β-alanine derivative products on spring oilseed rape yield and oil quality

“…DNA amplification was carried out in PCR tubes; the total reaction volume was 25 µl. DNA amplification was performed using a thermocycler Veriti® 96-Well Thermal Cycler (Applied Biosystems, USA) programmed to 1 cycle at 94°C for 5 min, following 35 cycles at 94°C for 30 s, at 42-44°C for 1 min, at 72°C for 1 min, final extension at 72°C for 1 min, and kept at 4°C (Jodinskienė et al, 2008). After amplification, PCR products were separated by electrophoresis in 1% agarose gel.…”

Genetic differentiation among sexually compatible relatives of Brassica napus L.

“…Their results demonstrated high efficiency of these markers for monitoring intra-specific genetic diversity among B. napus (distinguishing between winter and spring varieties) and inter-specific diversity. Investigations of B. napus genetic diversity by RAPD method were successfully applied in distinguishing between different varieties in Lithuania (JODINSKIENE et al, 2008). The occurrence and frequency of gene flow from oil varieties of B. napus (oilseed rape) and R. raphanistrum (wild radish) was assessed by development of crop-specific transposable element (SINE) markers (PRIETO et al, 2005).…”