Rapidly-Deposited Polydopamine Coating via High Temperature and Vigorous Stirring: Formation, Characterization and Biofunctional Evaluation

Abstract

Polydopamine (PDA) coating provides a promising approach for immobilization of biomolecules onto almost all kinds of solid substrates. However, the deposition kinetics of PDA coating as a function of temperature and reaction method is not well elucidated. Since dopamine self-polymerization usually takes a long time, therefore, rapid-formation of PDA film becomes imperative for surface modification of biomaterials and medical devices. In the present study, a practical method for preparation of rapidly-deposited PDA coating was developed using a uniquely designed device, and the kinetics of dopamine self-polymerization was investigated by QCM sensor system. It was found that high temperature and vigorous stirring could dramatically speed up the formation of PDA film on QCM chip surface. Surface characterization, BSA binding study, cell viability assay and antibacterial test demonstrates that the polydopamine coating after polymerization for 30 min by our approach exhibits similar properties to those of 24 h counterpart. The method has a great potential for rapid-deposition of polydopamine films to modify biomaterial surfaces.

Ultrasensitive microfluidic analysis of circulating exosomes using a nanostructured graphene oxide/polydopamine coating

“…For comparison, it was reported that the conventional processes under static or shaking conditions required >24 h to form a PDA coating thicker than 60 nm. 37, 38 The microfluidic deposition rate was comparable with that of a rapid PDA coating method using vigorous stirring at 60 °C. 38 In contrast to the dynamic microfluidic coating, we conducted static dopamine polymerization in microchannels for 10 h, which deposited a much thinner PDA film (<50 nm).…”

“…36, 37 However, most existing PDA coating methods are slow and require tens of hours to produce relatively thick surface coatings. 37, 38 Compared to these methods, our microfluidic coating approach markedly expedites the PDA deposition kinetics, which could promote the greater application of this promising coating material. 37, 38 …”

“…37, 38 Compared to these methods, our microfluidic coating approach markedly expedites the PDA deposition kinetics, which could promote the greater application of this promising coating material. 37, 38 …”

Probing polydopamine adhesion to protein and polymer films: microscopic and spectroscopic evaluation

“…1) [25,[44][45][46]. AFM probes were APTES-functionalised in a manner equivalent to Hong et al [47] and Lyubchenko et al [48], to act as a primer to further functionalisation; the probes were left in APTES vapour for at least 30 min.…”

Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

“…8 Recently, the polydopamine¯lm, which is a great binding media for surface modi¯cation on the inorganic and organic surfaces, 9 is pointed to be an economical and convenient solution for rapid surface functionalization. 10 The reported surface modi¯cation method for label free biosensors is usually operated by immersing the sensor chip in the dopamine aqueous solution to coat the polydopamine¯lm on the sensing surface using dopamine self-polymerization. 11,12 The polydopaminē lms are not only coated on the sensing surface of SPR chip but also the other surface due to good adhesion of dopamine, and the polydopamine¯lm on the surfaces of SPR chips apart from the sensing surface should be removed to avoid the adverse effect on measurement before it is attached to the experimental system.…”