2009
DOI: 10.1073/pnas.0911674106
|View full text |Cite
|
Sign up to set email alerts
|

Rapid β-lactam-induced lysis requires successful assembly of the cell division machinery

Abstract: ␤-lactam antibiotics inhibit penicillin binding proteins (PBPs) involved in peptidoglycan synthesis. Although inhibition of peptidoglycan biosynthesis is generally thought to induce cell lysis, the pattern and mechanism of cell lysis can vary substantially. ␤-lactams that inhibit FtsI, the only division specific PBP, block cell division and result in growth as filaments. These filaments ultimately lyse through a poorly understood mechanism. Here we find that one such ␤-lactam, cephalexin, can, under certain co… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

5
116
2

Year Published

2011
2011
2023
2023

Publication Types

Select...
6
2

Relationship

1
7

Authors

Journals

citations
Cited by 108 publications
(123 citation statements)
references
References 35 publications
5
116
2
Order By: Relevance
“…However, in SS996 host strains, these growing SS996 cells become more susceptible to antibiotic killing in ampicillin-selective media due to the fact that ampicillin and other β-lactam antibiotics kill only dividing cells [26]. This increased susceptibility to ampicillin was By enhancing the survival of the host cells in these unfavourable circumstances/growth conditions, the hok/sok locus would subsequently enhance the propagation of the resistance elements carried on the plasmid.…”
Section: Discussionmentioning
confidence: 99%
“…However, in SS996 host strains, these growing SS996 cells become more susceptible to antibiotic killing in ampicillin-selective media due to the fact that ampicillin and other β-lactam antibiotics kill only dividing cells [26]. This increased susceptibility to ampicillin was By enhancing the survival of the host cells in these unfavourable circumstances/growth conditions, the hok/sok locus would subsequently enhance the propagation of the resistance elements carried on the plasmid.…”
Section: Discussionmentioning
confidence: 99%
“…While treatment with relatively low levels of the FtsI-specific ␤-lactam cephalexin (10 g/ml) inhibits division, treatment with higher levels (50 g/ml) or with less-specific ␤-lactams like ampicillin results in rapid cell lysis from lesions that emanate from septa (14, 22, 40, 41, 53, 57, 60). Previous studies indicate that this rapid lysis is dependent upon all of the essential cell division proteins, including FtsN, as well as the LytM factors and the amidases (14,60). AmiA appeared to be the most important amidase for rapid lysis (14), suggesting that miscoordinated AmiA activation at the septum by EnvC may be the primary lytic determinant.…”
Section: Discussionmentioning
confidence: 99%
“…Curiously, an AmiB Ϫ AmiC Ϫ double mutant does not have a strong cell separation phenotype (14), suggesting that amidase localization is dispensable for cell separation. This indicates that AmiA can largely facilitate cell separation on its own even though it does not specifically accumulate at the division site.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Cells were grown into early exponential phase and transferred onto agarose pads for phase-contrast imaging on an inverted Nikon TE2000-E microscope. Details of the imaging acquisition were described previously (76). Computational analysis of single-cell size is implemented in MATLAB.…”
Section: Methodsmentioning
confidence: 99%