Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (<i>Danio rerio</i>)

Cong, Lin; Wang, Zeguan; Chai, Yuming; Hang, Wei; Shang, Chunfeng; Yang, Wenbin; Bai, Lu; Du, Jiulin; Wang, Kai; Wen, Quan

doi:10.1101/131532

Cited by 51 publications

(77 citation statements)

References 53 publications

(1 reference statement)

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“…In our laboratory, we have shown the application of LFM for whole-brain Ca 2+ imaging in small organisms like C. elegans and zebrafish larvae (Prevedel et al 2014). Recently, other variants of LFM have also been used to demonstrate whole-brain Ca 2+ imaging of freely swimming larval zebrafish (Cong et al 2017) and Drosophila (Aimon et al 2017). …”

Section: Categorization Of Current Microscopy Methods Used For Ca2mentioning

confidence: 99%

A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity

Weisenburger

Vaziri

2018

Annu. Rev. Neurosci.

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The mammalian brain is a densely interconnected network that consists of millions to billions of neurons. Decoding how information is represented and processed by this neural circuitry requires the ability to capture and manipulate the dynamics of large populations at high speed and resolution over a large area of the brain. While there has been a rapid increase in use of optical approaches in the neuroscience community over the last two decades, most microscopy approaches lack the ability to record the activity of all neurons comprising a functional network across the mammalian brain at relevant temporal and spatial resolution. In this review, we survey the recent development in the optical calcium imaging technologies in this regard and provide an overview of the strengths and limitations of each modality and their potential for scalability. We provide a guidance from a biological user perspective that is driven by the typical biological applications and sample conditions. We also discuss the potential for future advances and synergies that could be obtained through hybrid approaches or other modalities.

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Section: Categorization Of Current Microscopy Methods Used For Ca2mentioning

confidence: 99%

A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity

Weisenburger

Vaziri

2018

Annu. Rev. Neurosci.

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“…With advanced microfluidic systems to capture and recapture larval zebrafish, one can conduct more intense imaging on the same fish to achieve higher temporal resolution while minimizing the perturbations to the fish [28]. Using newly developed movable platform to track zebrafish larvae and novel volume imaging system, live imaging of cellular circadian rhythm on free-moving zebrafish will become possible [29]. In summary, our single-cell circadian reporter zebrafish line will have broad applications in circadian research.…”

Section: Discussionmentioning

confidence: 99%

Single-cell in vivo imaging reveals light-initiated circadian oscillators in zebrafish

Wang

Yang

et al. 2019

Preprint

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Circadian clock is a cell-autonomous time-keeping mechanism established gradually during embryonic development. Here we generated a transgenic zebrafish line carrying a destabilized fluorescent protein driven by the promoter of a core clock gene, nr1d1, to report in vivo circadian rhythm at the single-cell level. By time-lapse imaging of this fish line, we observed the sequential initiation of the reporter expression starting at photoreceptors in pineal gland then spreading to cells in other brain regions. Even within pineal gland, we found heterogeneous onset of nr1d1 expression in which each cell undergoes circadian oscillation superimposed over cell-type specific developmental trajectory. Furthermore, we found that single-cell expression of nr1d1 showed synchronous circadian oscillation under light-dark cycle. Remarkably, singlecell oscillations were lost in animals raised under constant darkness while developmental trend still persists. It suggests that light exposure in early clock development initializes cellular clocks rather than synchronizes existing individual oscillators as previously believed.

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“…Remarkably, we are already able to record from the majority of the neurons, at cellular resolution, in two animals during free behavior, C. elegans [67,68] and another invertebrate, Hydra [69], though progress is being made toward similar capabilities in adult Drosophila [70,71], larval Drosophila [72], and larval zebrafish [73]. For C. elegans , real-time quantification of movement drives the motion of a motorized stage that re-positions the worm directly under the neural imaging scope [67,68].…”

Section: Dissecting Circuits For Sensorimotor Behaviorsmentioning

confidence: 99%

Quantifying behavior to solve sensorimotor transformations: advances from worms and flies

Calhoun

Murthy

2017

Current Opinion in Neurobiology

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The development of new computational tools has recently opened up the study of natural behaviors at a precision that was previously unachievable. These tools permit a highly quantitative analysis of behavioral dynamics at timescales that are well matched to the timescales of neural activity. Here we examine how combining these methods with established techniques for estimating an animal’s sensory experience presents exciting new opportunities for dissecting the sensorimotor transformations performed by the nervous system. We focus this review primarily on examples from Caenorhabditis elegans and Drosophila melanogaster – for these model systems, computational approaches to characterize behavior, in combination with unparalleled genetic tools for neural activation, silencing, and recording, have already proven instrumental for illuminating underlying neural mechanisms.

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Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

Cited by 51 publications

References 53 publications

A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity

A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity

Single-cell in vivo imaging reveals light-initiated circadian oscillators in zebrafish

Quantifying behavior to solve sensorimotor transformations: advances from worms and flies

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