Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain

Price, Martin L.; Butler, Larry G.

doi:10.1021/jf60214a034

Cited by 693 publications

(417 citation statements)

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“…For each period, samples were pooled and oven dried at 608C for 72 h. Samples were milled and stored in plastic bags for further analyses. All the samples were analyzed to determine their dry matter (DM), ash, ether extract, CP (AOAC, 1980), NDF, ADF (Van Soest et al, 1991), ADF-N (Van Soest et al, 1991;AOAC, 2000), condensed tannins (Price et al, 1978) and total phenol (Price, 1977) content. The chemical composition of maize, grass and foliage is presented in Table 2.…”

Section: Fodder Intake and Metabolic Cost Of Parasites In Hair Sheepmentioning

confidence: 99%

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

Retama-Flores

Torres-Acosta

Sandoval-Castro

et al. 2012

Animal

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This trial evaluated the effect of maize supplementation on the ingestive behavior, nutrient intake and the resilience against gastrointestinal nematode (GIN) infection of hair sheep in a silvopastoral system containing tropical grasses and legume trees. In addition, it attempted to determine the metabolic cost of the natural GIN infection in supplemented and non-supplemented animals. Twenty-nine 3-month-old lambs (male and female), raised nematode free, were allocated to four groups: I-NS (infected, not supplemented, n = 8), I-S (infected, supplemented with maize at 1.5% live weight (LW), n = 7), T-NS (treated with moxidectin 0.2 mg/kg LW every 28 days, and not supplemented, n = 7) and T-S (treated with moxidectin and supplemented with maize at 1.5% LW, n = 7). During the 70-day trial, fodder intake, fodder selection, LW change (LWC), red blood cell counts (RBC), hemoglobin (Hb), hematocrit (Ht) and eggs per gram of feces (EPG) were measured every 14 days. Supplement consumption was recorded daily. Metabolizable energy (ME) and protein (MP) consumption from the feeds were estimated. Maize supplementation helped to improve the resilience of hair sheep lambs against GIN infections. The I-S and T-NS groups showed similar LWC, RBC, Hb and Ht (P > 0.05) and both were higher than those in the I-NS group (P < 0.05). No difference was found in EPG between the I-NS and the I-S groups (P > 0.05). No effect of sex was observed in the different variables. Although all groups showed low dry matter intake (DMI) (< 2% LW), supplemented groups (T-S and I-S) showed higher total DMI (fodder + maize; P < 0.05), hence higher ME and MP intakes than the non-supplemented groups (T-NS and I-NS). All groups showed similar fodder selection patterns. The estimated metabolic cost of parasitism was ME = 0.70 MJ/day and MP = 9.2 g/day in the I-S animals. Meanwhile, the cost in the I-NS animals was ME = 1.46 MJ/day and MP = 12.71 g/day. Maize supplementation was an economically viable strategy to control GIN compared with no intervention.

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Section: Fodder Intake and Metabolic Cost Of Parasites In Hair Sheepmentioning

confidence: 99%

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

Retama-Flores

Torres-Acosta

Sandoval-Castro

et al. 2012

Animal

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“…Esta prueba se basa en la reducción de los iones Fe 3+ a Fe 2+ , por los polifenoles mostrando una coloración azul, formando el complejo ferrocianuro-ferroso, registrándose una máxima absorbancia a 720 nm teniendo como ventaja: a) La rapidez y simplicidad de la prueba debido a la formación del color azul, lo que ofrece sensibilidad y versatilidad para la determinación espectrofotométrica, b) Los resultados son directamente medidos del contenido de polifenoles solubles (PRICE;BUTLER, 1977), obteniéndose en el extracto acuoso de la cáscara seca pintón de camu-camu, mayor contenido de polifenoles. Se realizó mediante el método de Azul de Prussian, reportado por Price y Butler (1977).…”

Section: Cuantificación De Polifenoles Totalesunclassified

Antocianinas, ácido ascórbico, polifenoles totales y actividad antioxidante, en la cáscara de camu-camu (Myrciaria dubia (H.B.K) McVaugh)

Villanueva-Tiburcio¹,

Condezo-Hoyos²,

Asquieri³

2010

Ciênc. Tecnol. Aliment.

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“…Method according Price and Butler (1977). In this method, phenolate anion is oxidised to phenolate radical and at the same time hexacyanoferrite (ferricyanide) ion is reduced to hexacyanoferrate (ferrocyanide) forming Prussian blue ) was modified to a semi micro-scale (reaction volume 1 ml).…”

Section: Chemicalsmentioning

confidence: 99%

Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods

Stratil¹,

Kubáň²,

Fojtová³

2008

Czech J. Food Sci.

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Stratil P., Kubáň V., Fojtová J. (2008): Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods. Czech J. Food Sci., 26: 242-253.Folin-Ciocalteu reagent (FCM) and Price and Butler method (PBM) were used for spectrophotometric determination of the total content of phenolic compounds in 29 wines (8 white, 21 red). The average contents of phenolic compounds determined by FCM and PBM were 108 (90-119) and 105 (90-129) for white wines, and 1545 (874-2262) and 547 (306-816) mg/l of gallic acid equivalents (GAE) for red wines, respectively. The reason for the lower PBM values in red wines is the higher reactivity in PBM of phenolic compounds, especially of gallic acid generally used as a standard in the above methods. The higher reactivity of the standard means that the measured absorbance of the sample responds to a lower concentration. The average total antioxidant activities determined by TEAC (Trolox Equivalent Antioxidant Capacity), FRAP (Ferric Reducing Antioxidant Power), and DPPH (using diphenyl-p-picrylhydrazyl radical) were 5.14 (4.30-6.14), 1.43 (0.86-2.14), and 0.71 (0.61-0.81) of Trolox equivalents (TE) and 26.44 (13. 9-34.4), 9.43 (4.92-13.9), and 5.52 (2.91-8.62) mmol/l TE for white and red wines, respectively. Almost the same molar absorptivities with TEAC and DPPH methods were found while with FRAP method it was somewhat higher (about 1.56-times). The ratio of the values determined by FRAP and DPPH methods for white and red wines were 2.0 and 1.7, respectively. The TEAC values were 2.8-and 4.8-fold higher than those determined by FRAP and DPPH methods, respectively. The radical ABTS •+ used in TEAC method is therefore the most reactive and responds to the highest number of hydroxyl groups of the phenolic compounds of wines.

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Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain

Cited by 693 publications

References 10 publications

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

Maize supplementation of Pelibuey sheep in a silvopastoral system: fodder selection, nutrient intake and resilience against gastrointestinal nematodes

Antocianinas, ácido ascórbico, polifenoles totales y actividad antioxidante, en la cáscara de camu-camu (Myrciaria dubia (H.B.K) McVaugh)

Comparison of the phenolic content and total antioxidant activity in wines as determined by spectrophotometric methods

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