Rapid UPLC–ESI–MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

Prokudina, E.A.; Havlı́cěk, Libor; Al‐Maharik, Nawaf; Lapčı́k, Oldřich; Strnad, Miroslav; Grúz, Jiří

doi:10.1016/j.jfca.2011.12.001

Cited by 56 publications

(43 citation statements)

References 29 publications

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“…For each molecule, the two most abundant fragments were selected as Q candidates. The fragmentation pathways of these two molecules appeared to be similar to those reported by Antignac et al (2003) and Prokudina et al (2012).…”

Section: Methods Developmentsupporting

confidence: 79%

“…For equol detection, MS detection techniques are the most widely used because they are very sensitive and can detect very low analyte content in biological fluids (Setchell and Clerici 2010a, b). LC-MS is now a fast-moving field, with many new detection and chromatographic techniques being developed over the past decade aimed at achieving higher sensitivity and reducing analysis time (Gumustas et al 2013;Prokudina et al 2012;Wu et al 2004). Currently UPLC ® -MS/MS analysis appears to be the fastest method; it is easiest to implement and produces reliable results.…”

Section: Introductionmentioning

confidence: 99%

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Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow’s milk

Daems

Jasselette

Romnee

et al. 2015

Dairy Sci. & Technol.

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Cow's milk can be used as a potential source of equol in the human diet. In order to study human intake, however, it is necessary to develop a reliable and sensitive analytical method. This paper reports on the validation of an analytical method using ultra-performance liquid chromatography coupled with a tandem mass spectrometry detector to quantify the equol in commercial milks (raw, whole, semi-skimmed, and skimmed milk). The equol was initially released using enzymatic hydrolysis, and it was then extracted using a double liquid/liquid extraction. The analytical method produced a linear calibration curve with a high correlation coefficient (R 2 ≥0.996) between 5 and 1,000 ng.mL -1 . Good intra-and inter-day precision (≤5.3% and≤5.2%, respectively) and accuracy (≤8.6%) were achieved. The recovery rate differed slightly among the different types of milk, ranging between 60.6± 1.09% and 82.3±5.21%. Good method repeatability was observed (≤15%). There was neither matrix effect nor carry-over effect, and the sample extracts were stable for at least 7 days of storage at -21 °C and 5 °C. The method proved to be specific, sensitive, precise, and accurate and was used for the first time to quantify the equol content in Belgian commercial cow's milk. In all the samples analyzed, equol was present at a concentration ≥10 ng.mL -1 and had a significantly higher content in organic than in conventional milk. The study also found that the mean concentrations of equol were similar for each type of commercial conventional cow's milk.

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Section: Methods Developmentsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow’s milk

Daems

Jasselette

Romnee

et al. 2015

Dairy Sci. & Technol.

View full text Add to dashboard Cite

show abstract

“…Several studies describe UPLC as being more sensitive than HPLC in the detection and quantification of antioxidant compounds from natural fi matrices such as ascorbic acid [203], vitamin E [204], procyanidins [205] and isoflavonoids [206]. fl High performance liquid chromatography-electrospray ionization mass spectrometry (HPLC/ESI-MS) is another chromatographic technique recently used for the detection and quantification of individual antifi oxidants [90].…”

Section: Chromatographic Techniquesmentioning

confidence: 99%

Determination of Antioxidant Compounds in Foodstuff

et al. 2016

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“…The separated isoflavones including aglycones, glycosides and glycoside malonates, were individually analyzed and identified by their molecular ions and characteristic fragment ion peaks using LC-MS under MS and MS/MS mode, in comparison with the standard isoflavones (Wu et al 2003). Prokudina et al (2012) used UPLC-ESI-MS/MS for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A, daidzin, genistin, ononin, sissotrin), 2 prenylated Isoflavonoids(osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarin (scopoletin). The application of this method was also demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and quantified (Prokudina et al 2012).…”

Section: Lc-ms/ms Analysis Of Isoflavonoidsmentioning

confidence: 99%

“…Prokudina et al (2012) used UPLC-ESI-MS/MS for the quantitative and qualitative analysis of 26 phenolic compounds in plant material, including 15 isoflavonoids (daidzein, genistein, isoformononetin, formononetin, prunetin, biochanin A, daidzin, genistin, ononin, sissotrin), 2 prenylated Isoflavonoids(osajin, pomiferin), 5 flavones (apigenin, kaempferol, quercetin, quercetin-3-glucoside, rutin), 4 flavanones (hesperetin, naringenin, naringenin-7-glucoside, naringin), a coumestan (coumestrol) and a coumarin (scopoletin). The application of this method was also demonstrated by the analysis of mung bean (Vigna radiata) sprout extract, in which 14 of the 26 analytes were detected and quantified (Prokudina et al 2012). Klejdus et al (2010) developed a hyphenated ultrasound assisted super critical fluid extraction method for the extraction and determination of isoflavones in sea and freshwater algae and cyanobacteria.…”

Section: Lc-ms/ms Analysis Of Isoflavonoidsmentioning

confidence: 99%

Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry

et al. 2015

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Isoflavonoids are the biologically active secondary metabolites of plants that are being used for several health promoting and restoring effects mediated through different pathways. Isoflavonoids are structurally similar to estrogens due to which also known as phytoestrogens and have shown potent estrogenic and anti-estrogenic activity. Association with large biological activity lead to the need of rapid, sensitive and precise quantitation of different isoflavonoids in different plant extracts, food materials and biological matrices as the biological activities mainly depends on the quantities and nature of isoflavonoids present in them. The characterisation, standardisation and quantification of herbal extracts or food products require techniques that are highly selective, sensitive and also provide mass measurement precisions and structural information. Liquid chromatography with tandem mass spectroscopy has made it possible to analyse and characterize several constituents and their metabolites in a single run along with high selectivity and sensitivity. In this review, we have summarised the application of LC-MS/MS for the identification and quantification of isoflavonoids reported for various plant extracts and food products along with their general extraction procedures and factors affecting extraction providing a view towards the conditions used for their analysis. The most suitable and widely acceptable extraction solvent system for the isoflavonoids is methanol or ethanol in combination with water ranging from 40 to 60 % organic solvent based on the type of tissues and the isoflavonoids to be extracted by different extraction techniques. ESI ionization with Q-TOF MS was the most useful detection system for the characterisation and quantification of the diverse isoflavonoids with molecular insights.

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Rapid UPLC–ESI–MS/MS method for the analysis of isoflavonoids and other phenylpropanoids

Cited by 56 publications

References 29 publications

Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow’s milk

Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow’s milk

Determination of Antioxidant Compounds in Foodstuff

Phytochemical analysis of isoflavonoids using liquid chromatography coupled with tandem mass spectrometry

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