Rapid Ultrasound Optical Clearing of Human Light and Dark Skin

Genina, Elina A.; Surkov, Yury I.; Serebryakova, Isabella A.; Bashkatov, Alexey N.; Tuchin, Valery V.; Zharov, Vladimir P.

doi:10.1109/tmi.2020.2989079

Cited by 22 publications

(19 citation statements)

References 49 publications

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“…OCA is not only also widely used for current laser-related diagnosis and treatment, but also used for ultrasound imaging and laser ablation. 9,12,18,19 In this study, we determined whether light scattering reducing substances could increase the treatment efficiency using a 1064 nm diode laser that could be applied to various intracutaneous diseases and musculoskeletal diseases.…”

Section: Discussionmentioning

confidence: 99%

The Effect of an Optical Clearing Agent on Tissue Prior to 1064-nm Laser Therapy

Youn

2021

Medical Lasers

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Section: Discussionmentioning

confidence: 99%

The Effect of an Optical Clearing Agent on Tissue Prior to 1064-nm Laser Therapy

Youn

2021

Medical Lasers

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“…These agents are commonly known as SOCAs (skin optical clearing agents) and generally adjust skin refractive indexes and induce packaging of scatterers such as collagen fibers ( Tuchina et al., 2019 ) by dehydration-driven reduction of skin thickness ( Wen et al., 2010 ). Several penetration enhancers are widely used to facilitate their diffusion and improve their clearing power; they can be classified into chemical penetration enhancers such as propylene glycol, DMSO ( Zaytsev et al., 2020 ), ethanol ( Zaytsev et al., 2020 ), azone ( Zhao et al., 2016 ), dimethyl sulfoxide ( Genina et al, 2020 ), oleic acid ( Zaytsev et al., 2020 ), and thiazone ( Zaytsev et al., 2020 ) (the last four ones achieve penetration enhancement by dissolving Stratum Corneum lipids) ( Zaytsev et al., 2020 ), and physical penetration enhancers, such as ultrasounds (sonophoresis) ( Genina et al, 2020 ), microneedles ( Yoon et al., 2010 ), lasers (FLMA, fractional laser microablation) ( Genina et al., 2020a ), pneumatic pressure ( Damestani et al., 2014 ), heat ( Damestani et al., 2014 ), or abrasive instruments (microdermabrasion) ( Genina et al, 2020 ). Different combinations of penetration enhancers have been applied to improve the efficacy of several clearing agents.…”

Section: Translational Applications Of Tissue Clearing and 3d Imagingmentioning

confidence: 99%

“…Glycerol has been applied to in vivo human skin with prior Stratum Corneum removal ( Fox et al., 2009 ), and 40% glucose solutions have been injected subcutaneously inducing long term increased transparency of in vivo human dermis ( Tuchin et al., 2001 ). Recently, a novel skin optical clearing method named MOUSE (Microdermabrasion, Oleic acid, and UltraSound Effect) has been successfully applied to human skin in vivo ( Genina et al, 2020 ), further increasing light penetration depth to 800 μm. Additionally, and as mentioned in section Tumor Tissue, optical clearing agents have been applied in vivo to human melanoma to image microvascular networks ( Pires et al., 2016 ).…”

Section: Optical Clearing and 2d And 3d Imaging As A Pathology Diagnomentioning

confidence: 99%

Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease

et al. 2020

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Summary Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.

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“…OCT is a noninvasive imaging technique based on low‐coherence interferometry. OCT enables label‐free, high‐speed 3D imaging of living tissue at cellular resolution, and has been increasingly adapted in clinical diagnosis and biomedical research (Ali et al., 2017; Genina et al., 2020; Katkar, Tadinada, Amaechi, & Fried, 2018). Combined with optical clearing, OCT exhibits 3D visualization of mouse embryonic structures with an imaging depth of >5 mm, and also enables 3D microvascular mapping in large tissue volumes (Choi, Maga, Kim, & Wang, 2020).…”

Section: Microscopy For Imaging Cleared Tissuesmentioning

confidence: 99%

Applications of tissue clearing in the spinal cord

Tian

2020

Eur J of Neuroscience

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The locomotor networks in the spinal cord coordinate muscle contractions and govern limb movements, ensuring postural body stability and smooth locomotion at different speeds. Dissecting the organization and connection of spinal motor networks is crucial to interpret their functional roles and thus can help to design more specific interventions for motor system diseases. Traditional two‐dimensional (2D) histological sectioning is inadequate to accurately dissect complex architecture of the spinal cord as it provides only partial spatial information about spinal neural circuits. It is particularly difficult for quantitative analysis of axon regeneration after injury with 2D tissue sections, because it shows axonal fragments rather than spatial trajectory of regenerating axons. Therefore, three‐dimensional (3D) imaging and analysis are extremely necessary for investigations of spinal cord structure and function. Although 3D spatial structure of the spinal cord can be reconstructed by serial sectioning, this approach is laborious and prone to image distortion. The recently emerging tissue clearing technique enables 3D imaging of the entire spinal cord at cellular resolution without tissue sectioning. The development of tissue clearing contributes to revealing the organization and function of spinal circuits and elucidating associated mechanisms underlying certain behaviours in health and disease. In this paper, we give an overview of the current clearing methods and introduce available labelling and imaging techniques as well as data processing software. Finally, we demonstrate the recent applications of tissue clearing in the spinal cord. Tissue clearing technique provides a novel tool for 3D imaging and quantification of the spinal cord, and benefits investigations of structure–function relationship of spinal networks. This review might help researchers to find the potential of tissue clearing in the studies of spinal cord and select appropriate clearing protocol for their experimental schemes.

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Rapid Ultrasound Optical Clearing of Human Light and Dark Skin

Cited by 22 publications

References 49 publications

The Effect of an Optical Clearing Agent on Tissue Prior to 1064-nm Laser Therapy

The Effect of an Optical Clearing Agent on Tissue Prior to 1064-nm Laser Therapy

Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease

Applications of tissue clearing in the spinal cord

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