Rapid turnover of intra-host genetic diversity in Zucchini yellow mosaic virus

Simmons, H. E.; Holmes, Edward C.; Stephenson, Andrew G.

doi:10.1016/j.virusres.2010.11.007

Cited by 13 publications

(14 citation statements)

References 57 publications

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“…The methods used for PCR cloning and Sanger sequencing of the coat protein region of the samples SG1 and SG2 are as previously described (Simmons et al, 2011b). …”

Section: Methodsmentioning

confidence: 99%

Zucchini yellow mosaic virus (ZYMV, Potyvirus): Vertical transmission, seed infection and cryptic infections

et al. 2013

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The role played by seed transmission in the evolution and epidemiology of viral crop pathogens remains unclear. We determined the seed infection and vertical transmission rates of zucchini yellow mosaic virus (ZYMV), in addition to undertaking Illumina sequencing of nine vertically transmitted ZYMV populations. We previously determined the seed-to-seedling transmission rate of ZYMV in Cucurbita pepo ssp. texana (a wild gourd) to be 1.6%, and herein observed a similar rate (1.8%) in the subsequent generation. We also observed that the seed infection rate is substantially higher (21.9%) than the seed-to-seedling transmission rate, suggesting that a major population bottleneck occurs during seed germination and seedling growth. In contrast, that two thirds of the variants present in the horizontally transmitted inoculant population were also present in the vertically transmitted populations implies that the bottleneck at vertical transmission may not be particularly severe. Strikingly, all of the vertically infected plants were symptomless in contrast to those infected horizontally, suggesting that vertical infection may be cryptic. Although no known virulence determining mutations were observed in the vertically infected samples, the 5’ untranslated region was highly variable, with at least 26 different major haplotypes in this region compared to the two major haplotypes observed in the horizontally transmitted population. That the regions necessary for vector transmission are retained in the vertically infected populations, combined with the cryptic nature of vertical infection, suggests that seed transmission may be a significant contributor to the spread of ZYMV.

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“…The methods used for PCR cloning and Sanger sequencing of the coat protein region of the samples SG1 and SG2 are as previously described (Simmons et al, 2011b). …”

Section: Methodsmentioning

confidence: 99%

Zucchini yellow mosaic virus (ZYMV, Potyvirus): Vertical transmission, seed infection and cryptic infections

et al. 2013

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“…; Simmons et al. ,b). These methods require either well‐equipped laboratories or complicated procedures.…”

Section: Discussionmentioning

confidence: 99%

“…) and seeds (Simmons et al. ) of cucurbitaceous plants. In addition, ELISA or RT‐PCR was used to study seed transmission rates, and genetic diversity of ZYMV (Simmons et al.…”

Section: Introductionmentioning

confidence: 99%

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Use of Reverse Transcription Loop‐Mediated Isothermal Amplification for the Detection of Zucchini yellow mosaic virus

Kuan

Deng

Huang

et al. 2013

Journal of Phytopathology

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A Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) assay was employed to develop a simple and efficient system for the detection of Zucchini yellow mosaic virus (ZYMV) in squash and melon plants. The RT-LAMP assay took 30 min under isothermal condition at 64°C by employing a set of four primers targeting ZYMV. The sensitivity of RT-LAMP was 10-fold greater than that of the RT-PCR assay in the detection of ZYMV in infected tissues of squash and melon. No reaction was detected from the tissues of healthy plants by either RT-LAMP or RT-PCR assay. The RT-LAMP product of the tested samples can be visualized by staining directly in the tube with SYBR Green I dye. The sensitivity of SYBR Green I staining method is similar to that analyzed by gel electrophoresis. Field-grown squash and melon plants were tested using RT-PCR and RT-LAMP. Both RT-LAMP and PCR could detect ZYMV in symptomatic or symptomless tissues of infected plants. However, the RT-LAMP assay is superior to RT-PCR because it is rapid, simple, and highly sensitive; therefore, RT-LAMP is a useful and practical method for detection of ZYMV in cucurbits.

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“…Given sufficient time, the selection regime in this type of interaction is altered and tends to increase genetic and phenotypic variability. Increased variability promotes the emergence of generalists and the possibility of new ecological opportunities (Best et al, 2010;Gilman et al, 2012;Hall et al, 2011;Paterson et al, 2010;Scanlan et al, 2011;Simmons et al, 2011;Thompson, 2012;Yoder and Nuismer, 2010).…”

Section: Chapter 1 General Introductionmentioning

confidence: 99%

Phylogeny of the subfamily Streblinae (Diptera: Streblidae) and historical host-parasite association

Alcântara¹

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Rapid turnover of intra-host genetic diversity in Zucchini yellow mosaic virus

Cited by 13 publications

References 57 publications

Zucchini yellow mosaic virus (ZYMV, Potyvirus): Vertical transmission, seed infection and cryptic infections

Zucchini yellow mosaic virus (ZYMV, Potyvirus): Vertical transmission, seed infection and cryptic infections

Use of Reverse Transcription Loop‐Mediated Isothermal Amplification for the Detection of Zucchini yellow mosaic virus

Phylogeny of the subfamily Streblinae (Diptera: Streblidae) and historical host-parasite association

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