Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin

Sumida, Mizuki; Hane, Masaya; Yabe, Uichiro; Shimoda, Yasushi; Pearce, Oliver M.T.; Kiso, Makoto; Miyagi, Taeko; Sawada, Makoto; Varki, Ajit; Kitajima, Ken; Sato, Chihiro

doi:10.1074/jbc.m115.638759

Cited by 83 publications

(88 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, Neu5Gc is incorporated into NCmahTg polySia as well as Neu5Ac, and yet apparently it results in no gross defect in brain development. Of course, this does not rule out the possibility that neuronal development might be affected at the cellular level or that the release of brain-derived neurotrophic factor or growth factors bound on polySia might be impaired (19).…”

Section: Resultsmentioning

confidence: 99%

“…PolySia modulates cell-cell interactions, thus playing important roles in neuronal development and regeneration (15)(16)(17). Recently, it was suggested that polySia also serves as a reservoir of growth factors (18,19). Genetic studies have revealed that variation in a gene encoding the enzyme that biosynthesizes polySia, ST8SIA2, could be a risk factor of psychiatric disorders such as schizophrenia, autism spectrum, and bipolar disorder (20 -23).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Physiological Exploration of the Long Term Evolutionary Selection against Expression of N-Glycolylneuraminic Acid in the Brain

Naito-Matsui

Davies

Takematsu

et al. 2017

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Edited by Amanda J. FosangAll vertebrate cell surfaces display a dense glycan layer often terminated with sialic acids, which have multiple functions due to their location and diverse modifications. The major sialic acids in most mammalian tissues are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), the latter being derived from Neu5Ac via addition of one oxygen atom at the sugar nucleotide level by CMP-Neu5Ac hydroxylase (Cmah). Contrasting with other organs that express various ratios of Neu5Ac and Neu5Gc depending on the variable expression of Cmah, Neu5Gc expression in the brain is extremely low in all vertebrates studied to date, suggesting that neural expression is detrimental to animals. However, physiological exploration of the reasons for this long term evolutionary selection has been lacking. To explore the consequences of forced expression of Neu5Gc in the brain, we have established brain-specific Cmah transgenic mice. Such Neu5Gc overexpression in the brain resulted in abnormal locomotor activity, impaired object recognition memory, and abnormal axon myelination. Brain-specific Cmah transgenic mice were also lethally sensitive to a Neu5Gc-preferring bacterial toxin, even though Neu5Gc was overexpressed only in the brain and other organs maintained endogenous Neu5Gc expression, as in wild-type mice. Therefore, the unusually strict evolutionary suppression of Neu5Gc expression in the vertebrate brain may be explained by evasion of negative effects on neural functions and by selection against pathogens.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Physiological Exploration of the Long Term Evolutionary Selection against Expression of N-Glycolylneuraminic Acid in the Brain

Naito-Matsui

Davies

Takematsu

et al. 2017

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Desialylation on the cell surface by sialidase can change cell surface properties quickly and dramatically. Neu1 is also contributed to the desialylation on the cell surface in the central nervous system [11]. Neu3, plasma-membrane-bound sialidase, cleaves sialic acid bound to the major brain gangliosides that are necessary for memory function.…”

Section: Discussionmentioning

confidence: 99%

“…Since exogenous sialidase extracellularly applied to the hippocampus influences memory and synaptic plasticity [9, 10], endogenous sialidase activity in the extracellular space would also affect the hippocampal memory processing. In the case of inflammatory response in the central nervous system, sialylation level with PSA on microglial cells was regulated by exovesicular sialidase [11]. Mammalian sialidase isozymes are designated as Neu1, Neu2, Neu3 and Neu4.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of Sialidase in Long-Term Potentiation at Mossy Fiber-CA3 Synapses and Hippocampus-Dependent Spatial Memory

et al. 2016

View full text Add to dashboard Cite

Sialic acid bound to glycans in glycolipids and glycoproteins is essential for synaptic plasticity and memory. Sialidase (EC 3.2.1.18), which has 4 isozymes including Neu1, Neu2, Neu3 and Neu4, regulates the sialylation level of glycans by removing sialic acid from sialylglycoconjugate. In the present study, we investigated the distribution of sialidase activity in rat hippocampus and the role of sialidase in hippocampal memory processing. We previously developed a highly sensitive histochemical imaging probe for sialidase activity, BTP3-Neu5Ac. BTP3-Neu5Ac was cleaved efficiently by rat Neu2 and Neu4 at pH 7.3 and by Neu1 and Neu3 at pH 4.6. When a rat hippocampal acute slice was stained with BTP3-Neu5Ac at pH 7.3, mossy fiber terminal fields showed relatively intense sialidase activity. Thus, the role of sialidase in the synaptic plasticity was investigated at mossy fiber terminal fields. The long-term potentiation (LTP) at mossy fiber-CA3 pyramidal cell synapses was impaired by 2,3-dehydro-2-deoxy-N-acetylneuraminic acid (DANA), a sialidase inhibitor. DANA also failed to decrease paired-pulse facilitation after LTP induction. We also investigated the role of sialidase in hippocampus-dependent spatial memory by using the Morris water maze. The escape latency time to reach the platform was prolonged by DANA injection into the hippocampal CA3 region or by knockdown of Neu4 without affecting motility. The results show that the regulation of sialyl signaling by Neu4 is involved in hippocampal memory processing.

show abstract

Biochemical Characterization and Analyses of Polysialic‐Acid‐Associated Carrier Proteins and Genes in Piglets during Neonatal Development

et al. 2017

View full text Add to dashboard Cite

show abstract

Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin

Cited by 83 publications

References 55 publications

Physiological Exploration of the Long Term Evolutionary Selection against Expression of N-Glycolylneuraminic Acid in the Brain

Physiological Exploration of the Long Term Evolutionary Selection against Expression of N-Glycolylneuraminic Acid in the Brain

Role of Sialidase in Long-Term Potentiation at Mossy Fiber-CA3 Synapses and Hippocampus-Dependent Spatial Memory

Biochemical Characterization and Analyses of Polysialic‐Acid‐Associated Carrier Proteins and Genes in Piglets during Neonatal Development

Contact Info

Product

Resources

About