Rapid transformation and plant regeneration of sorghum (Sorghum bicolor L.) mediated by altruistic Baby boom and Wuschel2

Nelson‐Vasilchik, Kimberly; Hague, Joel; Tilelli, Michael; Kausch, Albert P.

doi:10.1007/s11627-021-10243-8

Cited by 11 publications

(8 citation statements)

References 48 publications

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“…Alternative approaches have been reported to exclude MR expression late in development. These approaches rely on transient expression of MRs without integration in combination with selection of the T-DNA of interest (Hoerster et al, 2020;Aregawi et al, 2021;Nelson-Vasilchik et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

et al. 2022

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Plant transformation is a bottleneck for the application of gene editing in plants. In Zea mays (maize), a breakthrough was made using co-transformation of the morphogenic transcription factors BABY BOOM (BBM) and WUSCHEL (WUS) to induce somatic embryogenesis. Together with adapted tissue culture media, this was shown to increase transformation efficiency significantly. However, use of the method has not been reported widely, despite a clear need for increased transformation capacity in academic settings. Here, we explore use of the method for the public maize inbred B104 that is widely used for transformation by the research community. We find that only modifying tissue culture media already boosts transformation efficiency significantly and can reduce the time in tissue culture by 1 month. On average, production of independent transgenic plants per starting embryo increased from 1 to 4% using BIALAPHOS RESISTANCE (BAR) as a selection marker. In addition, we reconstructed the BBM-WUS morphogenic gene cassette and evaluated its functionality in B104. Expression of the morphogenic genes under tissue- and development stage-specific promoters led to direct somatic embryo formation on the scutellum of zygotic embryos. However, eight out of ten resulting transgenic plants showed pleiotropic developmental defects and were not fertile. This undesirable phenotype was positively correlated with the copy number of the morphogenic gene cassette. Use of constructs in which morphogenic genes are flanked by a developmentally controlled Cre/LoxP recombination system led to reduced T-DNA copy number and fertile T0 plants, while increasing transformation efficiency from 1 to 5% using HIGHLY-RESISTANT ACETOLACTATE SYNTHASE as a selection marker. Addition of a CRISPR/Cas9 module confirmed functionality for gene editing applications, as exemplified by editing the gene VIRESCENT YELLOW-LIKE (VYL) that can act as a visual marker for gene editing in maize. The constructs, methods, and insights produced in this work will be valuable to translate the use of BBM-WUS and other emerging morphogenic regulators (MRs) to other genotypes and crops.

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Section: Discussionmentioning

confidence: 99%

Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

et al. 2022

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“…Elevated expression of BBM-like PLT gene members, such as PLT1, PLT2, PLT3, and PLT7, has been demonstrated to induce somatic embryogenesis. Notably, PLT1 and PLT3 were found to recover early-stage embryo defects of plt2 bbm mutants, suggesting a redundant and overlapping role of BBM and BBM-like genes in Boutilier et al, 2002;Srinivasan et al, 2007;Deng et al, 2009;Heidmann et al, 2011;Florez et al, 2015;Mookkan et al, 2017;Mookkan et al, 2017;Yavuz et al, 2020;Nelson-Vasilchik et al, 2022;Qi et al, 2022;Khanday et al, 2023;Xiao et al, 2023, Chen et…”

Section: Ap2/erf Familymentioning

confidence: 99%

“…Aregawi et al, 2020;Hoerster et al, 2020;Peterson et al, 2021;Aregawi et al, 2022;Beyene et al, 2022, Xu et al, 2022b, Nelson-Vasilchik et al, 2022Johnson et al, 2023;Wang et al, 2023, …”

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Multifaceted roles of transcription factors during plant embryogenesis

Yuan,

Kagale,

Ferrie

2024

Front. Plant Sci.

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Transcription factors (TFs) are diverse groups of regulatory proteins. Through their specific binding domains, TFs bind to their target genes and regulate their expression, therefore TFs play important roles in various growth and developmental processes. Plant embryogenesis is a highly regulated and intricate process during which embryos arise from various sources and undergo development; it can be further divided into zygotic embryogenesis (ZE) and somatic embryogenesis (SE). TFs play a crucial role in the process of plant embryogenesis with a number of them acting as master regulators in both ZE and SE. In this review, we focus on the master TFs involved in embryogenesis such as BABY BOOM (BBM) from the APETALA2/Ethylene-Responsive Factor (AP2/ERF) family, WUSCHEL and WUSCHEL-related homeobox (WOX) from the homeobox family, LEAFY COTYLEDON 2 (LEC2) from the B3 family, AGAMOUS-Like 15 (AGL15) from the MADS family and LEAFY COTYLEDON 1 (LEC1) from the Nuclear Factor Y (NF-Y) family. We aim to present the recent progress pertaining to the diverse roles these master TFs play in both ZE and SE in Arabidopsis, as well as other plant species including crops. We also discuss future perspectives in this context.

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“…Nevertheless, the possibility of generation of transformed quinoa plants through Agrobacterium -mediated transformation is not a vague reality. This technique can be improved further by using the booster genes such as WUSCHEL , BABY BOOM and LEAFY COTYLEDON1 which have been previously known to improve the transformation efficiency in other crops like maize and sorghum ( Nelson-Vasilchik et al, 2022 ). The de novo induction of meristems could also be an alternative approach along with the expression of booster genes to avoid complications in tissue-culture strategies.…”

Section: Genome Editing For Underutilized Dryland Crops: Progress And...mentioning

confidence: 99%

CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects

Sharma

Palakolanu²,

Bhattacharya³

et al. 2022

Front. Genet.

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Technologies and innovations are critical for addressing the future food system needs where genetic resources are an essential component of the change process. Advanced breeding tools like “genome editing” are vital for modernizing crop breeding to provide game-changing solutions to some of the “must needed” traits in agriculture. CRISPR/Cas-based tools have been rapidly repurposed for editing applications based on their improved efficiency, specificity and reduced off-target effects. Additionally, precise gene-editing tools such as base editing, prime editing, and multiplexing provide precision in stacking of multiple traits in an elite variety, and facilitating specific and targeted crop improvement. This has helped in advancing research and delivery of products in a short time span, thereby enhancing the rate of genetic gains. A special focus has been on food security in the drylands through crops including millets, teff, fonio, quinoa, Bambara groundnut, pigeonpea and cassava. While these crops contribute significantly to the agricultural economy and resilience of the dryland, improvement of several traits including increased stress tolerance, nutritional value, and yields are urgently required. Although CRISPR has potential to deliver disruptive innovations, prioritization of traits should consider breeding product profiles and market segments for designing and accelerating delivery of locally adapted and preferred crop varieties for the drylands. In this context, the scope of regulatory environment has been stated, implying the dire impacts of unreasonable scrutiny of genome-edited plants on the evolution and progress of much-needed technological advances.

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Rapid transformation and plant regeneration of sorghum (Sorghum bicolor L.) mediated by altruistic Baby boom and Wuschel2

Cited by 11 publications

References 48 publications

Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

Multifaceted roles of transcription factors during plant embryogenesis

CRISPR for accelerating genetic gains in under-utilized crops of the drylands: Progress and prospects

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