Rapid tool for identification of bacterial strains using Fourier transform infrared spectroscopy on genomic DNA

Abstract

Aims We developed a new rapid and reliable method for identifying bacteria using a combination of Fourier transform infrared (FT‐IR) spectroscopy of bacterial genomic DNA and multivariate analysis. Methods and Results FT‐IR spectra of genomic DNA from four type strains of Pseudomonas spp., three type strains of Escherichia spp. and two type strains of Bacillus spp. were analysed in the 4000–400 cm−1 region. Spectral differences were found in the frequency regions of N–H stretching (amide I), C=O stretching vibrations (amide II) and PO2− ionized asymmetric and symmetric stretching. Partial least squares discriminant analysis of the FT‐IR spectra showed that the microbial strains could be discriminated by hierarchical clustering analysis. Conclusions FT‐IR spectral analysis of bacterial genomic DNA has potential for the rapid identification of bacteria at the genus and species levels. Significance and Impact of the Study This study reports a new bacterial identification method using multivariate analysis of FT‐IR spectra of bacterial genomic DNA.

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