Rapid techniques for diagnostic and epidemiological studies of rotavirus infection

Giordano, Miguel O.; Basnec, S.N.; Nates, Silvia; Bennun, Fernando Ruben; Depetris, Ariel

doi:10.1016/0166-0934(91)90085-e

Cited by 7 publications

(3 citation statements)

References 13 publications

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“…In 1978, Muchinik et al conducted the first study to determine the frequency of rotavirus in patients with acute diarrhea admitted to the "Ricardo Gutiérrez" Children's Hospital of Buenos Aires (15)(16)(17). Since then, a variety of other studies developed by several groups have evaluated different aspects of rotavirus epidemiology in different settings (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35).…”

mentioning

confidence: 99%

Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina

Ja¹,

Nates²,

Nr³

et al. 1998

Rev Panam Salud Publica

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mentioning

confidence: 99%

Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina

Ja¹,

Nates²,

Nr³

et al. 1998

Rev Panam Salud Publica

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“…PAGE. In order to confirm the presence of the virus, roots of DAS-ELISA positive plants were analyzed with polyacrylamide gel electrophoresis (PAGE) for the detection of RNA according to the Giordano et al protocol (11) modified by Giménez Pecci (8). Positive and negative controls were DAS-ELISA-positive maize and DAS-ELISA-negative wheat plants, respectively.…”

Section: Methodsmentioning

confidence: 99%

Wheat: A New Natural Host for the Mal De Río Cuarto Virus in the Endemic Disease Area, Río Cuarto, Córdoba Province, Argentina

et al. 1998

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The fijivirus known as “Mal de Río Cuarto” that affects corn is endemic to the area of Río Cuarto, Cordoba Province, Argentina. One of the preferred species for the development of its vector, the insect Delphacodes kuscheli Fennah, is wheat. In this area, wheat plants with deformed leaves, spikes and spikelets, shortened internodes, leaves with serrated borders, and sterile spikelets were detected, suggesting the possibility that Mal de Río Cuarto Virus could also be infecting this crop. Samples originating in Río Cuarto, Sampacho, and La Carlota (Córdoba Province) that showed symptoms, were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polyacrylamide gel electrophoresis, and electron microscopy, confirming, for the first time, the occurrence of the disease in wheat. The frequency of the disease was assessed in random samples from 14 wheat plots located in the Department of Río Cuarto (Córdoba Province). The samples were analyzed using the DASELISA immunoenzymatic technique, and the disease was detected in the majority of the fields assessed, with levels of incidence that ranged between 2.5 and 24%. We must be aware of the presence of this virus in the wheat crop, where it appears to play a double role in the epidemiology of the disease, acting both as a virus reservoir and as a preferred host for the development of populations of the vector virus, D. kuscheli.

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“…En 1978, Muchinik et al realizaron el primer estudio para determinar la frecuencia de rotavirus en pacientes con diarrea aguda que ingresaron al Hospital de Niños "Ricardo Gutiérrez" de Buenos Aires (15,16). Desde entonces, una serie de estudios adicionales realizados por varios grupos han evaluado distintos aspectos de la epidemiología de los rotavirus en diferentes medios (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35).…”

Section: Introductionunclassified

En anticipación de una vacuna antirrotavirus: revisión de estudios epidemiológicos sobre la diarrea por rotavirus en la Argentina

Gómez¹,

Nates²,

Castagnaro³

et al. 1998

Rev Panam Salud Publica

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Rapid techniques for diagnostic and epidemiological studies of rotavirus infection

Cited by 7 publications

References 13 publications

Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina

Anticipating rotavirus vaccines: review of epidemiologic studies of rotavirus diarrhea in Argentina

Wheat: A New Natural Host for the Mal De Río Cuarto Virus in the Endemic Disease Area, Río Cuarto, Córdoba Province, Argentina

En anticipación de una vacuna antirrotavirus: revisión de estudios epidemiológicos sobre la diarrea por rotavirus en la Argentina

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