Rapid spectroscopic confirmation of the presence of polystyrene in polymer particles of mixed composition

Crawford, Kevin D.; Hughes, Kenneth D.

doi:10.1002/(sici)1099-0488(19980430)36:6<999::aid-polb8>3.0.co;2-b

Cited by 1 publication

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References 5 publications

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“…Other groups have applied fluorescence spectroscopy for the characterization of single molecules, which were separated from a trapped polystyrene sphere by means of a liquid flow. The amount of analyte present in the investigated particle could thus be determined 39–41…”

Section: Introductionmentioning

confidence: 99%

Identification of Biotic and Abiotic Particles by Using a Combination of Optical Tweezers and In Situ Raman Spectroscopy

et al. 2004

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A highly versatile setup, which introduces an optical gradient trap into a Raman spectrometer, is presented. The particular configuration, which consists of two lasers, makes trapping independent from the Raman excitation laser and allows a separate adjustment of the trapping and excitation wavelengths. Thus, the excitation wavelength can be chosen according to the needs of the application. We describe the successful application of an optical gradient trap on transparent as well as on reflective, metal-coated microparticles. Raman spectra were recorded from optically trapped polystyrene beads and from single biological cells (e.g., erythrocytes, yeast cells). Also, metal-coated microparticles were trapped and used as surface enhanced Raman spectroscopy (SERS) substrates for tests on yeast cells. Furthermore, the optical gradient trap was combined with a SERS fiber probe. Raman spectra were recorded from trapped red blood cells using the SERS fiber probe for excitation.

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Section: Introductionmentioning

confidence: 99%