Rapid somatic embryogenesis from the nucelli of monoembryonic mango varieties

Jana, M.; Nadgauda, R. S.; Rajmohan, K.; Mascarenhas, A. F.

doi:10.1007/bf02632120

Cited by 12 publications

(4 citation statements)

References 5 publications

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“…Also, due to lack of vascular connection with the parent plant, nucellus is considered free of pathogens, and therefore the plants having a nucellar origin would be disease-free. Among fruit trees, somatic embryogenesis from nucellus has been reported in mango (Litz et al, 1982(Litz et al, , 1984Litz, 1984;Jana et al, 1994), citrus (Rangan et al, 1968(Rangan et al, , 1969Button and Bornman, 1971;Kochba et al, 1972;Button et al, 1974;Navarro et al, 1985;Perez et al, 1998), grape (Mullins and Srinivasan, 1976), redcurrant (Zatyko et al, 1975) and apple (Eichholtz et al, 1979). Recently, induction of somatic embryogenesis has been reported from the nucellar tissue of cashewnut (Ananthakrishnan et al, 1999), using liquid medium for the induction of somatic embryos.…”

Section: Resultsmentioning

confidence: 94%

Induction of somatic embryogenesis in cashewnut (Anacardium occidentale L.)

Gogte

Nadgauda

2000

In Vitro Cell.Dev.Biol.-Plant

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Somatic embryogenesis was induced in callus cultures derived from nucellar tissue of cashewnut (Anacardium occidentale L.). Callus was obtained from nucellar tissue after 3 wk of culture on semisolid Murashige and Skoog (MS) basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 5 mM) ϩ gibberellic acid (GA 3 , 15 mM) ϩ N 6 -benzyladenine (BA, 5 mM). This callus gave rise to an embryogenic mass after 9 wk on maintenance medium containing 2, 4-D (10 mM) ϩ GA 3 (15 mM) ϩ 4% sucrose ϩ 0.5% activated charcoal ϩ 10% coconut water (CW) ϩ 0.05% casein hydrolysate (CH). The embryogenic mass, after transfer to medium supplemented with 2,4-D (5 mM) ϩ GA 3 (30 mM) ϩ 4% sucrose ϩ 0.5% activated charcoal ϩ 10% CW ϩ 0.05% CH, gave rise to somatic embryos. The developmental stages of somatic embryos were observed using light and stereo microscopes. Histological study of somatic embryo development was also carried out. The present study would be useful for clonal propagation, and variety improvement in cashewnut, which is essential due to its increasing demand and export potential.

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Section: Resultsmentioning

confidence: 94%

Induction of somatic embryogenesis in cashewnut (Anacardium occidentale L.)

Gogte

Nadgauda

2000

In Vitro Cell.Dev.Biol.-Plant

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“…The results of the present study indicate that explants of small size fruits gave significantly the highest number of callused explants and primary somatic embryos compared to medium size (Table 2). In another study, nucellar tissues from immature mango fruit of size 2.5 to 4.5 cm long were used to induce somatic embryogenesis in cultivar Alphonso (Deore et al, 2000) while rapid production of somatic embryos with normal morphology and germination were achieved in monoembryonic mango cultivars Alphonso, Mundan and Baneshan from the nucellar explants (Jana et al, 1994). An interaction between explants size and cultivars significantly affected on the number of explants callused.…”

Section: Effect Of Explants Size On Callus and Primary Somatic Embryomentioning

confidence: 99%

Nucellar embryogenesis and plantlet regeneration in monoembryonic and polyembryonic mango (Mangifera indica L.) cultivars

Shukla¹,

Al-Busaidi²,

Al-Blushi³

et al. 2016

Afr. J. Biotechnol.

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Biotic and abiotic stress particularly fungal diseases and salinity are major challenges facing mango cultivations in Oman. Micropropagation technique for multiplying disease resistant and salinity tolerant elite cultivars could be utilized to replace dead and infected plants in mango orchards but standardize in-vitro regeneration protocol via somatic embryogenesis is prerequisite. Nucellar tissues from immature mango fruits of monoembryonic cultivars Alphonso, Amrapali, Dashehari and Zafran, and polyembryonic cultivars Carabao and Turpentine were used as explants to induce somatic embryogenesis plantlets. Gamborg's B5 macronutrients, Murashige and Skoog micronutrients, iron source, vitamins and organics were used as standard basal media for all types of media used at each stage of somatic embryo development and regeneration. Induction medium 2 containing 2 mg/l 2,4-Dichlorophenoxyacetic acid and 0.5 mg/l 6-Benzylaminopurine were induced highest percentage of primary somatic embryos for Alphonso (22.08%) while induction medium 3 having 1 mg/l 2,4-Dichlorophenoxyacetic acid with sucrose 60 gm/l and induction medium 1 containing 1 mg/l 2,4-Dichlorophenoxyacetic acid and 0.25 mg/l 6-Benzylaminopurine induced highest percentage of primary somatic embryos in Carabao (29.17%) and Turpentine (42.71%) respectively. Maximum somatic embryo germination were achieved in germination medium 2 containing 0.1 mg/l Indole-3-acetic acid and 0.5 mg/l Gibberellic acid for Alphonso (7.34%) and Turpentine (3.34%) while for Carabao (18.59%) in germination medium 1 which does not contain any plant growth regulators. Germinated plantlets are surviving well in ex-vitro conditions after 4 months of transfer to greenhouse and survival rate of 66.66% for Alphonso, 26.68% for Carabao and 49.16% for Turpentine was obtained.

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“…Somatic embryogenesis has been demonstrated in both polyembryonic (Litz et al, 1982;Dewald et al, 1989a,b) and monoembryonic mango (Litz, 1984;Jana et al, 1994;Thomas, 1999). The efficiency of regeneration from nucellar tissue of mango has been shown to be genotype-dependent in both polyembryonic (Litz et al, 1982) and monoembryonic cultivars (Litz, 1984;Jana et al, 1994;Litz et al, 1998).…”

mentioning

confidence: 96%

“…The efficiency of regeneration from nucellar tissue of mango has been shown to be genotype-dependent in both polyembryonic (Litz et al, 1982) and monoembryonic cultivars (Litz, 1984;Jana et al, 1994;Litz et al, 1998).…”

mentioning

confidence: 99%