Rapid, single-step differentiation of equid herpesviruses 1 and 4 from clinical material using the polymerase chain reaction and virus-specific primers

Lawrence, Glenda; Gilkerson, James R.; Love, Daria N.; Sabine, Margaret; Whalley, J. M.

doi:10.1016/0166-0934(94)90066-3

Cited by 73 publications

(57 citation statements)

References 24 publications

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“…The samples were diagnosed as EHV-1 infected by restriction fragment length polymorphism of viral DNA [5], PCR for detection of the EHV-1 gC gene [18], or immunohistochemical staining. One hundred eighty-one samples were homogenates of lung or thymus collected from equine fetuses from 109 cases of sporadic abortions or epizootic abortion outbreaks in which no simultaneous neurological disease was registered.…”

Section: )mentioning

confidence: 99%

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Tsujimura

Oyama

Katayama

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G 2254 ) in the Hidaka district-a major horse breeding area in Japan-we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G 2254 strains. This prevalence is lower than those observed in the U.S

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Section: )mentioning

confidence: 99%

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Tsujimura

Oyama

Katayama

et al. 2011

The Journal of Veterinary Medical Science

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“…The presence of EHV-1 DNA was confirmed both in the material obtained directly from the aborted foal placenta as well as in the material from the 6 th passage of the obtained virus isolate on the RK13 cell line using the species-specific polymerase chain reaction (PCR) as described by Lawrence et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine Herpesvirus 1 and changes caused by serial passage in vitro

Molínková

2012

Acta Vet. Brno

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The aim of this work was to isolate the abortogenic virus strain of Equine Herpesvirus 1 representing the current infection situation in the Czech Republic, describe it at the molecular level with accent on genes coding viral glycoproteins and observe the changes caused by the passaging of the virus on a cell culture. In 2009, an isolate of equine herpesvirus 1 was obtained from an abortion case in a mare from a herd affected by abortion storm. The virus identification was performed using the PCR method. The virus was isolated on the RK 13 cell line and after 6 passages in vitro the stability of sequences of selected sections of the virus genome was assessed and compared with the original field isolate. The virus sequences were also compared with known sequences of the abortogenic reference virus strain (V592) and with other known viral strains. One point mutation in a nucleotide sequence coding glycoprotein G was found, distinguishing the field isolate from V592. One point mutation in the gene for glycoprotein C was passage-induced. It was noted that the virus during the passage on RK13 cell line in the monitored sections was stable and is a suitable starting material for next experiments. EHV-1, viral abortion in mares, glycoproteins, genom

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“…Amplification of viral DNA by PCR can be further enhanced by hybridisation with viral specific DNA probes (Lawrence et al, 1994;Welch et al, 1992;Kirisawa et al, 1993) and also by restriction endonuclease digestion (Allen et al, 1983;Morris and Field, 1988;Palfi and Christensen, 1995). However, the extraction of viral DNA from samples may also lead to the co-purification of PCR inhibitors (Zerbini et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Originally EHV-1 was designated as rhinopneumonitis virus, however examination of restriction endonuclease DNA fingerprints by Studdert et al (1981) showed two distinct virus subtypes namely EHV-1 and EHV-4. While EHV-4 is restricted to the respiratory epithelium and lymph nodes draining the lung, EHV-1 is distinct in that it spreads systemically resulting in post-respiratory complications including abortion and paralysis (Wagner et al, 1992;Lawrence et al, 1994). Due to the ability of EHV-1 to cause 'abortion storms' in mares after fetal infection (Studdert and Blackney 1979;Crabb and Studdert, 1995) diagnosis of EHV-1 must be rapid and sensitive so that early intervention policies, aimed at reducing the effect of virus spread, can be put in place (Ballagi-Pordany et al, 1990;Sharma et al, 1992;Lawrence et al, 1994;Gupta et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

The development of a competitive PCR–ELISA for the detection of equine herpesvirus-1

Daly

Doyle

2003

Journal of Virological Methods

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Equine herpesvirus-1 (EHV-1) infection is of significant animal welfare and economic importance. Yet, no standardised molecular techniques are available for diagnosis or confirmation of viral infection. The purpose of this study was to develop a standardised and quantitative assay system for the reliable detection of EHV-1 infection which was capable of eliminating the likelihood of false negative results. A region within the EHV-1 glycoprotein B gene was amplified by polymerase chain reaction (PCR), cloned and subjected to site-directed mutagenesis to generate a control plasmid, amplifiable by identical primers to wild type EHV-1, yet capable of detection by an alternate dinitrophenylated oligonucleotide probe in a PCR Á/ELISA system. A competitive PCR Á/ELISA system which can control for the presence of PCR inhibitors and which is capable of detecting 63 genome equivalents of EHV-1 has been developed. EHV-1 presence in infected equine tissue and cell culture material was demonstrated using this system. The entire assay can be completed within one working day and facilitates multiple sample analysis. The availability of a robust, competitive PCR Á/ELISA system for the detection of EHV-1 will facilitate the rapid and sensitive detection of EHV-1 and offers the potential for eliminating the occurrence of abortion storms in stud farms. #

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Rapid, single-step differentiation of equid herpesviruses 1 and 4 from clinical material using the polymerase chain reaction and virus-specific primers

Cited by 73 publications

References 24 publications

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine Herpesvirus 1 and changes caused by serial passage in vitro

The development of a competitive PCR–ELISA for the detection of equine herpesvirus-1

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