Rapid, simultaneous determination of lopinavir and ritonavir in human plasma by stacking protein precipitations and salting-out assisted liquid/liquid extraction, and ultrafast LC–MS/MS

Myasein, Francis; Kim, Elaine; Zhang, Jun; Wu, Huaiqin; El-Shourbagy, Tawakol A.

doi:10.1016/j.aca.2009.08.010

Cited by 71 publications

(33 citation statements)

References 17 publications

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“…LLE with water-miscible organic solvents had so far been reported for analysis of biological samples [23,24,33]. It can improve the recovery and applicability and greatly reduce the extraction time.…”

Section: Salting Out Assisted Liquid-liquid Extractionmentioning

confidence: 99%

Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry

Song

Ediage

et al. 2013

Journal of Chromatography A

104

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(2013). Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry. Journal of Chromatography A, 1292Chromatography A, , 111-120. 10.1016Chromatography A, /j.chroma.2012 AbstractDirect determination of urinary mycotoxins is a better approach to assess individual's exposure than the indirect estimation from average dietary intakes. In this study, a new analytical method was developed and validated for simultaneous analysis of aflatoxin B 1 , deoxynivalenol, fumonisin B 1 , ochratoxin A, zearalenone and T2 toxin and their metabolites in pig urine. In total 12 analytes were selected. A salting-out assisted liquid-liquid extraction procedure was used for sample preparation. High performance liquid chromatography tandem mass spectrometry was used for the separation and detection of all the analytes. The extraction recoveries were in a range of 70% to 108%, with the intra-day relative standard deviation and inter-day relative standard deviation of lower than 25% for most of the compounds at 3 different concentration levels. Meanwhile method bias for all the analytes did not exceed 20%. The method limits of quantification ranged from 0.07 ng mL -1 for ochratoxin A to 3.3 ng mL -1 for deoxynivalenol. Matrix effect was evaluated in this study and matrixmatched calibration was used for quantification. The developed method was also validated for human urine as an extension of its application. Finally, the developed method was applied in a pilot study to analyze 28 pig urine samples. Deoxynivalenol, aflatoxin B 1 , fumonisin B 1 and ochratoxin A were detected in these samples.

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Section: Salting Out Assisted Liquid-liquid Extractionmentioning

confidence: 99%

Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry

Song

Ediage

et al. 2013

Journal of Chromatography A

104

View full text Add to dashboard Cite

show abstract

“…It uses water-miscible organic solvents that, generally, have low toxicity and small amounts of salt that cause little environmental pollution. Additionally, this method has the advantages of simplicity and sensitivity and uses less solvents, and the product is compatible for subsequent analysis by HPLC (Myasein, Kim, Zhang, Wu, & Tawakol, 2009;Cai et al, 2007). In SALLME, a glass centrifuge tube is often used as the extraction device.…”

Section: Introductionmentioning

confidence: 99%

A phase separation method for analyses of fluoroquinones in meats based on ultrasound-assisted salt-induced liquid–liquid microextraction and a new integrated device

Wang

Gao

et al. 2015

Meat Science

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Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted, saltinduced, liquid-liquid microextraction for determination of five fluoroquinones in meats by HPLC analysis. The novel integrated device consisted of three simple HDPE (high density polyethylene) parts that were used to separate the solvent from the aqueous solution prior to retrieving the extractant. The extraction parameters were optimized using the response surface method based on central composite design: 589 μL of acetone solvent, pH 2.1, 4.1 min extraction time and 3.5 g of Na 2 SO 4 . The limits of detection were 0.056-0.64 μg kg −1 and recoveries were 87.2-110.6% for the five fluoroquinones in muscle tissue from fish, chicken, pork and beef. This method is easily constructed from inexpensive materials, extraction efficiency is high, and the approach is compatible with HPLC analysis. Thus, it has excellent prospects for sample pre-treatment and analysis of fluoroquinones in meat samples.

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“…It uses water-miscible organic solvents that, generally, have low toxicity and small amounts of salt that cause little pollution. Additionally, this method has the advantages of being simple and sensitive as well as using less solvents, and the product is compatible for subsequent analysis by HPLC (Cai et al, 2007;Myasein, Kim, Zhang, Wu, & Tawakol, 2009). In SALLME, a glass centrifuge tube is often used as the extraction device.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a phase separation based magnetic-stirring salt-induced liquid–liquid microextraction method for determination of fluoroquinolones in food

Gao

Wang

et al. 2015

Food Chemistry

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a b s t r a c tHerein, we developed a novel integrated apparatus to perform phase separation based on magneticstirring, salt-induced, liquid-liquid microextraction for determination of five fluoroquinolones in animal-based foods by HPLC analysis. The novel integrated apparatus consisted of three simple HDPE (high density polyethylene) parts that were used to separate the solvent from the aqueous solution prior to retrieving the extractant. The extraction parameters were optimized using the response surface method based on central composite design: 791 lL of acetone solvent, 2.5 g of Na 2 SO 4 , pH 1.7, 3.0 min of stir time, and 5.5 min centrifugation. The limits of detection were 0.07-0.53 lg kg À1 and recoveries were 91.6-105.0% for the five fluoroquinolones from milk, eggs and honey. This method is easily constructed from inexpensive materials, extraction efficiency is high, and the approach is compatible with HPLC analysis. Thus, it has excellent prospects for sample pre-treatment and analysis of fluoroquinolones in animal-based foods.

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Rapid, simultaneous determination of lopinavir and ritonavir in human plasma by stacking protein precipitations and salting-out assisted liquid/liquid extraction, and ultrafast LC–MS/MS

Cited by 71 publications

References 17 publications

Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry

Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry

A phase separation method for analyses of fluoroquinones in meats based on ultrasound-assisted salt-induced liquid–liquid microextraction and a new integrated device

Optimization of a phase separation based magnetic-stirring salt-induced liquid–liquid microextraction method for determination of fluoroquinolones in food

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