Rapid simultaneous determination of baclofen and its γ-hydroxy metabolite in urine by high-performance liquid chromatography with ultraviolet detection

Wuis, E.W.; Beijsterveldt, L E C van; Dirks, Rita; Vree, T. B.; Kleyn, E. Van Der

doi:10.1016/0378-4347(87)80176-7

Cited by 21 publications

(5 citation statements)

References 3 publications

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“…The minimum detectable concentration was 0.02mg.1-1. High urinary baclofen concentrations were determined, using the rapid direct-UV method [7]. Plasma protein binding in a number of plasma samples was measured at room temperature by filtration through EMIT | Free level filters (Syva, Pale Alto, USA).…”

Section: Analysis Of Plasma and Urinementioning

confidence: 99%

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Wuis

Dirks

Vree

et al. 1990

Pharmaceutisch Weekblad Scientific Edition

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The pharmacokinetics of racemic baclofen as determined from plasma and urine data in six spastic patients treated with individualized oral doses, 30-80 mg daily, are presented. Peak plasma concentrations were achieved 1.9 h (+/- 0.7) after a dose. The fluctuation in the plasma concentration was great, ranging from 188 to 439%. The total body clearance averaged 175 ml.min-1 (+/- 44), plasma protein binding 35% (+/- 6). Baclofen was for the greater part excreted unchanged by the kidney, 65% (+/- 16). Its apparent renal equalled the creatinine clearance. The contribution of the renal clearance to the total body clearance can explain the previously described toxicity when renal impairment is present. The results agree with earlier reports on single doses in healthy subjects.

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Section: Analysis Of Plasma and Urinementioning

confidence: 99%

Pharmacokinetics of baclofen in spastic patients receiving multiple oral doses

Wuis

Dirks

Vree

et al. 1990

Pharmaceutisch Weekblad Scientific Edition

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show abstract

“…To date, a number of analytical methods have been developed for determination of baclofen including gas chromatography (GC) [14][15][16][17][18], high-performance liquid chromatography (HPLC) [19][20][21][22][23][24][25][26][27], capillary electrophoresis (CE) [28], and liquid chromatography-tandem mass spectrometry (LC/MS/MS) [29][30][31]. However, most of the methods are not sensitive enough to be utilized for pharmacokinetic studies or require complex sample preparation procedures such as derivatization, liquid-liquid extraction, ion-pair extraction, solid phase extraction, ultra filtration.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry determination of baclofen in various biological samples and application to a pharmacokinetic study

Kim

Shin

et al. 2013

Journal of Chromatography B

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“…Although many methods based on gas chromatographyliquid chromatography (GC-LC) [13,14], HPLC [15][16][17][18], or MS [19,20] have been reported for the determination of baclofen in biologic fluids, a few successful enantioselective methods for baclofen analysis in biologic materials exist. Spahn et al [8] developed an enantiospecific HPLC method to determine S(+)-naproxen-derivatized baclofen enantiomers in human plasma, urine, and CSF; Sioufi et al [9] employed a chiral capillary column to separate N-heptafluorobutyryl isobutyl ester derivatives of baclofen enantiomers in human plasma and urine; and Chiang et al [10,11] described an enantiospecific method based on capillary electrophoretic (CE) separation of naphthalene-2,3-dicarboxaldehyde (NAD)-derivatized baclofen enatiomers.…”

Section: Discussionmentioning

confidence: 99%