Rapid, simple and sensitive high-performance liquid chromatographic method for detection and determination of acyclovir in human plasma and its use in bioavailability studies

Development and validation of a new reversed-phase ion pairing liquid chromatographic method with fluorescence detection for penciclovir analysis in plasma and aqueous humor

“…Conventional liquid-liquid extraction (LLE) could not be used since penciclovir is very polar (log P = −2.03) [47]. Direct injection after proteins precipitation with either an organic solvent or an acid was described for penciclovir related compounds such as aciclovir and ganciclovir [14][15][16][17][18][19][20][21][22][23][24][25][26][27]. However, injection of strongly acid solutions reduces analytical column lifetime, and leads to numerous late-eluting peaks disturbing the analytical procedure [22].…”

“…In order to enhance method selectivity and retention factor of penciclovir, an ion-pairing agent, sodium 1-octanesulfonate, was added to the mobile phase. In previous studies [14,24,28,[30][31][32][33], ionpairing agents (sodium octane-, heptane-and dodecylsulfonate) were often used at concentrations between 5 and 10 mM for nucleoside analogues. In our case, 5 mM sodium octanesulfonate allowed adequate retention of penciclovir and ganciclovir (k = 2.3 and 5.9, respectively).…”

Development and Validation of LC-MS/MS Method for the Analysis of P-Toluenesulfonicacid, In Antiviral Drug Acyclovir

“…Some of the analytical methods have been reported for acyclovir such as luorescence [18][19][20] direct UV [21][22][23][24] and HPLC-MS [25]. In that numerous HPLC methods have been reported for estimation of acyclovir in pharmaceutical formulations has been reported [26][27][28][29][30][31][32][33][34]. Among chromatographic techniques; the reversed-phase (RP) HPLC was widely used for the analysis.…”

Determination of acyclovir in horse plasma and body fluids by high‐performance liquid chromatography combined with fluorescence detection and heated electrospray ionization tandem mass spectrometry

“…For the pretreatment of plasma samples, some authors have used deproteinizing agents such as perchloroacetic acid followed by centrifugation and injection of the supernatant Stagni et al, 2004;Bangaru et al, 2000;Boulieu et al, 1997;Pham-Huy et al, 1999;Peh and Yuen, 1997;Jankowski et al, 1998). Some published studies describe the use of solid-phase extraction, resulting in extracts that are free of impurities and with high rates of drug recovery (Fernández et al, 2003;Poirier et al, 1999;Svensson et al, 1997;Kishino et al, 2002;Trottet et al, 2005).…”