Rapid serotyping of enteropathogenic Yersinia Enterocolitica strains by co-agglutination

Fernández-Lago, Luis; Rodriguez-Nebreda, M.S.; Chordi, A

doi:10.1016/0769-2609(88)90108-1

Annales de l'Institut Pasteur / Microbiologie

1988

DOI: 10.1016/0769-2609(88)90108-1

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Rapid serotyping of enteropathogenic Yersinia Enterocolitica strains by co-agglutination

Luis Fernández-Lago

M.S. Rodriguez-Nebreda

A Chordi

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1991

1992

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Cited by 2 publications

(1 citation statement)

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“…In recent years several authors have reported a marked increase in the frequency of infections due to Yersinia enterocolitica (12). Symptoms commonly ascribed to these infections include acute ileitis and mesenteric lymphadenitis, in rare cases complicated by septicemia (36).…”

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confidence: 99%

Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay

et al. 1991

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An enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide (S-LPS) as the antigen was used to analyze the antibody response in rabbits orogastrically and intravenously infected with virulent (plasmid-bearing) Yersinia enterocolitica O9 strains (pYV+) and with the avirulent (plasmid-cured) derivatives (pYV-). A significative response of immunoglobulin G (IgG), IgA, and IgM antibodies against the S-LPS antigen was evident in sera from the rabbits orogastrically infected with pYV+ strains. This immune response was stronger and persisted longer than those obtained with the corresponding pYV- strains. In contrast, few differences were observed in the titers and evolution of IgG, IgA, and IgM antibodies against the S-LPS antigen in rabbits intravenously infected with pYV+ and pYV- strains. These results suggest that the necessity of the virulence plasmid for the establishment of infection by Y. enterocolitica serotype O9 is conditioned by the infection route used. When the S-LPS ELISA was compared with the radial immunodiffusion test using the native hapten as the antigen, the results showed that the ELISA technique was more sensitive. However, only those sera obtained between 2 and 8 weeks postinfection from rabbits intravenously infected with plasmid-bearing strains were positive in the radial immunodiffusion test.

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Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay

et al. 1991

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A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody

Vizcaı́no

Fernández-Lago

1992

Research in Microbiology

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Rapid serotyping of enteropathogenic Yersinia Enterocolitica strains by co-agglutination

Cited by 2 publications

References 33 publications

Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay

Class-specific immune response to Yersinia enterocolitica serotype O9 antigens as determined by enzyme-linked immunosorbent assay

A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody

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