Rapid serological test for diagnosis of chlamydial ocular infections.

Darougar, S; Treharne, J D; Minassian, DC; El-Sheikh, H.; Dines, R. J.; Jones, Barrie R.

doi:10.1136/bjo.62.8.503

Cited by 45 publications

(8 citation statements)

References 10 publications

(8 reference statements)

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“…Still, the increasing evidence of the persistent and chronic character of chlamydia infections (Beatty et al 1994) motivates to test the hypothesis of their pathogenic role in VKC. The reason for selecting serology as a diagnostic method was, besides the availability of the material, the documented rise in circulating IgG in acute paratrachoma (Darougar et al 1978;Herrmann et al 1991) and in chronic trachoma (Holland et al 1993). In view of these data, we believe that our negative findings concerning serum antibody titers to C. trachomatis are relevant.…”

Section: Discussionmentioning

confidence: 78%

Vernal keratoconjunctivitis in a Stockholm ophthalmic centre ‐ epidemiological, functional, and immunologic investigations

Montan

Ekström²,

Hedlin³

et al. 1999

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To classify a cohort of 62 patients with vernal keratoconjunctivitis (VKC) in immunologic, functional, and epidemiological terms. Methods: A retrospective chart review was conducted to establish the patients' ethnic origin and to ascertain the results of standard in vitro and in vivo testing for atopic allergy. The latter data were compared with tear Phadiatop A , an allergy screening test, in 31 subjects. Further subgroup analyses included methacholine bronchial provocations, serum screening for chlamydial antibodies and epidemiological calculations of the observed prevalence of VKC in the Stockholm area in 1994. Results: Thirty-seven subjects (59.7%) were sensitised to common allergens. No additional allergic subjects were diagnosed with the tear Phadiatop A test. The serology for ocular chlamydial disease was negative. Only 6 out of 17 subjects displayed bronchial reactivity to methacholine of whom 4 had a history of asthma. VKC was clearly more common in individuals with an Asian and African origin. Conclusions: Sensitivity to allergens is a strong determinant for the disease but in a large proportion of the subjects this immunologic abnormality is absent. The varying prevalence of the condition in different ethnic groups indicates a genetic predisposing factor.

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Section: Discussionmentioning

confidence: 78%

Vernal keratoconjunctivitis in a Stockholm ophthalmic centre ‐ epidemiological, functional, and immunologic investigations

Montan

Ekström²,

Hedlin³

et al. 1999

Acta Ophthalmologica Scandinavica

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“…Recent studies have shown that the modified micro-immunofluorescence (micro-IF) test for the detection of different immunoglobulin classes of type-specific antichlamydial antibody can provide information on the prevalence of these infections (Treharne et al, 1977a). Studies on ocular chlamydial infections have shown that the detection of different immunoglobulin classes of type-specific chlamydial antibody in eye secretions (tear fluids) as well as in sera can be used successfully for diagnosis (Treharne et al, 1977b;Darougar et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of chlamydial infection of the cervix.

Treharne¹,

Darougar²,

Simmons³

et al. 1978

Sexually Transmitted Infections

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A rapid serodiagnostic test for the presumptive diagnosis of chlamydial infection of the cervix has been developed. The method used is based on the modified micro-immunofluorescence test using pooled chlamydial antigens and the detection of different immunoglobulin classes of chlamydial antibody in sera and cervical secretions. The presence of IgG chlamydial antibody at a level of 1/64, or IgM antibody at a level of 1/8 or greater, or both in sera and IgG or IgA antibody at a level of 1/8 or more or both in cervical secretions was closely associated with the isolation of Chlamydia trachomatis and non-specific genital infection. In general, serodiagnosis was three to nine times more sensitive than cultural methods, and the detection of IgG chlamydial antibody in cervical secretions alone provided the most sensitive of the serological tests. This sensitive, low-cost, rapid, and simple serodiagnostic test for the presumptive diagnosis of chlamydial infection of the cervix, coupled with transportation of specimens by post, offers advantages over conventional isolation techniques for the routine diagnosis and management of chlamydial genital infections.

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“…Studies of tear immunglobulins using the same animal model, however, revealed a change by three weeks after infection from a predominance of IgM to either IgG or IgA [10]. Antichlamydial IgG and IgA were also the predominant immunoglobulin types (with levels of IgG greater than those of IgA) detected in the tears of patients with active trachoma [11,12]. In the present study, IgG and IgA, with small amounts of IgM, were also found in the subepithelial conjunctival tissue and occasionally IgG and IgA were seen on the epithelial surface.…”

Section: Discussionmentioning

confidence: 99%

Conjunctival lymphocyte subsets in trachoma

et al. 1988

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Monoclonal antibodies were used to assess the lymphocyte populations in conjunctival biopsy specimens obtained from patients with trachoma (active or inactive) undergoing tarsotomy for the correction of trachoma-induced entropion and in three control patients. Peroxidase-labelled monoclonal antibodies OKT4 (identifies T-helper/inducer lymphocytes), OKT8 (identifies T-suppressor/cytotoxic lymphocytes), OKIal (identifies B-lymphocytes) and antisera specific for IgG, IgA and IgM were used to identify lymphocyte subpopulations and immunoglobulins in the conjunctival biopsy specimens. When grouped by disease activity, conjunctival tissue specimens revealed predominant T-helper/inducer lymphocytes in the substantia propria of patients with active trachoma while inactive trachoma patients had predominant T-suppressor/cytotoxic lymphocytes in the conjunctival biopsy specimens. B-lymphocytes were seen in moderate numbers in all conjunctival biopsy specimens from patients with active trachoma and all specimens from active cases stained for IgG, IgM, and IgA. Immunoglobulin staining was strongest with IgG and IgM.

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Rapid serological test for diagnosis of chlamydial ocular infections.

Cited by 45 publications

References 10 publications

Vernal keratoconjunctivitis in a Stockholm ophthalmic centre ‐ epidemiological, functional, and immunologic investigations

Vernal keratoconjunctivitis in a Stockholm ophthalmic centre ‐ epidemiological, functional, and immunologic investigations

Rapid diagnosis of chlamydial infection of the cervix.

Conjunctival lymphocyte subsets in trachoma

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