Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry

Condina, Mark R.; Dilmetz, Brooke A; Bazaz, Sajad Razavi; Meneses, Jon; Warkiani, Majid Ebrahimi; Hoffmann, Peter

doi:10.1039/c9lc00152b

Cited by 58 publications

(38 citation statements)

References 49 publications

(49 reference statements)

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“…These microfluidic techniques are grounded on the unique characteristics of microscale flow phenomena and have recently gained prominence as efficient tools for the control and focusing of microbeads. Amongst existing microfluidic systems, inertial microfluidics has experienced massive growth in many applications ranging from cell separation 7,8 , cytometry 9,10 , multiplexed bio-assays 11,12 , and also fluid mixing 13 . Despite great advantages of inertial microfluidics, the commercial impact and scalability of this technology have been restricted due to fabrication issues.…”

mentioning

confidence: 99%

3D Printing of Inertial Microfluidic Devices

Bazaz

Rouhi

Raoufi

et al. 2020

Sci Rep

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141

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Inertial microfluidics has been broadly investigated, resulting in the development of various applications, mainly for particle or cell separation. Lateral migrations of these particles within a microchannel strictly depend on the channel design and its cross-section. Nonetheless, the fabrication of these microchannels is a continuous challenging issue for the microfluidic community, where the most studied channel cross-sections are limited to only rectangular and more recently trapezoidal microchannels. As a result, a huge amount of potential remains intact for other geometries with cross-sections difficult to fabricate with standard microfabrication techniques. In this study, by leveraging on benefits of additive manufacturing, we have proposed a new method for the fabrication of inertial microfluidic devices. In our proposed workflow, parts are first printed via a high-resolution DLP/SLA 3D printer and then bonded to a transparent PMMA sheet using a double-coated pressure-sensitive adhesive tape. Using this method, we have fabricated and tested a plethora of existing inertial microfluidic devices, whether in a single or multiplexed manner, such as straight, spiral, serpentine, curvilinear, and contraction-expansion arrays. Our characterizations using both particles and cells revealed that the produced chips could withstand a pressure up to 150 psi with minimum interference of the tape to the total functionality of the device and viability of cells. As a showcase of the versatility of our method, we have proposed a new spiral microchannel with right-angled triangular cross-section which is technically impossible to fabricate using the standard lithography. We are of the opinion that the method proposed in this study will open the door for more complex geometries with the bespoke passive internal flow. Furthermore, the proposed fabrication workflow can be adopted at the production level, enabling large-scale manufacturing of inertial microfluidic devices.

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confidence: 99%

3D Printing of Inertial Microfluidic Devices

Bazaz

Rouhi

Raoufi

et al. 2020

Sci Rep

Self Cite

141

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“…where U max indicates the maximum fluid velocity, ρ is the fluid density, µ denotes the dynamic viscosity of the fluid, C L is the lift force coefficient, D h represents the channel hydraulic diameter, a is the particle diameter, and De is Dean number. Considering these equations, a particle (or cell) of the diameter a experiences F L and F d different uniquely determined by the a [11]. As schematically shown in Figure 1, the larger cells (prostate cancer cells) are focussed at the inner wall (where its height is purpose-designed to be smaller than that of the outer wall), whereas the smaller cells and debris drift to the outer wall.…”

Section: Spiral Microfluidic Devicementioning

confidence: 99%

“…Recently, isolation of tumour cells from biological samples via microfluidic technology has gained significant attention. Among the existing microfluidic modalities, inertial microfluidics based on a spiral microchannel device configuration has experienced a massive growth as exemplified by the separation of CTCs and circulating fetal trophoblasts [7][8][9][10], identification of bacterial spoilage from beers [11], blood fractionation [12], enrichment of circulating head and neck tumour cells [13], and separation of microalgae [14]. The technique is attractive for its cost-effectiveness and high throughput.…”

Section: Introductionmentioning

confidence: 99%

Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics

Rzhevskiy

Bazaz

Ding

et al. 2019

Cancers

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During the last decade, isolation of circulating tumour cells via blood liquid biopsy of prostate cancer (PCa) has attracted significant attention as an alternative, or substitute, to conventional diagnostic tests. However, it was previously determined that localised forms of PCa shed a small number of cancer cells into the bloodstream, and a large volume of blood is required just for a single test, which is impractical. To address this issue, urine has been used as an alternative to blood for liquid biopsy as a truly non-invasive, patient-friendly test. To this end, we developed a spiral microfluidic chip capable of isolating PCa cells from the urine of PCa patients. Potential clinical utility of the chip was demonstrated using anti-Glypican-1 (GPC-1) antibody as a model of the primary antibody in immunofluorescent assay for identification and detection of the collected tumour cells. The microchannel device was first evaluated using DU-145 cells in a diluted Dulbecco’s phosphate-buffered saline sample, where it demonstrated >85 (±6) % efficiency. The microchannel proved to be functional in at least 79% of cases for capturing GPC1+ putative tumour cells from the urine of patients with localised PCa. More importantly, a correlation was found between the amount of the captured GPC1+ cells and crucial diagnostic and prognostic parameter of localised PCa—Gleason score. Thus, the technique demonstrated promise for further assessment of its diagnostic value in PCa detection, diagnosis, and prognosis.

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“…10 5 cells/min could thereby be separated using five different outlets. In a different experiment by Condina et al 45 , spiral microchannels were used to separate beer spoilage bacteria from yeast for subsequent identification using mass spectrometry. Separation efficiencies of >90% were reached at a flow rate of 1.5 ml/min.…”

Section: Removal Of Contaminants In Microalgae Cell Culturesmentioning

confidence: 99%

Spiral microfluidic devices for cell separation and sorting in bioprocesses

2019

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Inertial microfluidic systems have been arousing interest in medical applications due to their simple and cost-efficient use. However, comparably small sample volumes in the microliter and milliliter ranges have so far prevented efficient applications in continuous bioprocesses. Nevertheless, recent studies suggest that these systems are well suited for cell separation in bioprocesses because of their facile adaptability to various reactor sizes and cell types. This review will discuss potential applications of inertial microfluidic cell separation systems in downstream bioprocesses and depict recent advances in inertial microfluidics for bioprocess intensification. This review thereby focusses on spiral microchannels that separate particles at a moderate Reynolds number in a laminar flow (Re < 2300) according to their size by applying lateral hydrodynamic forces. Spiral microchannels have already been shown to be capable of replacing microfilters, extracting dead cells and debris in perfusion processes, and removing contaminant microalgae species. Recent advances in parallelization made it possible to process media on a liter-scale, which might pave the way toward industrial applications.

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Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry

Abstract: Microfluidics and MALDI-TOF MS is a rapid, high-throughput, and accurate method for the identification of beer spoilage bacteria.

Cited by 58 publications

References 49 publications

3D Printing of Inertial Microfluidic Devices

3D Printing of Inertial Microfluidic Devices

Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics

Spiral microfluidic devices for cell separation and sorting in bioprocesses

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