Rapid Separation and Determination of 12 Kinds Phenolic Acids and Isoflavone in Iris Pseudoacorus Based on Ultra-performance Convergence Chromatography

Abstract

A method was developed for the rapid separation and determination of 12 kinds phenolic acids and isoflavones in Iris pseudoacorus based on Ultra performance convergene chromatography. The compounds were separated on a Waters Acquity UPC2 HSS(100mm×3. 0mm,1. 7μm) column at 30 °C by using CO2 and acetonitrile as the mobile phase at a flow rate of 0. 4mL/min, and then analyzed by a UV detector at wavelength of 270nm, the whole analysis progress was only 36 min. The results showed that the limits of detection (LOD) and the limits of quantitation (LOQ) of 12 compounds were between 0. 051 and 0. 18 μg/mL, and 0. 19 and 0. 63μg/mL, respectively. The spiked recoveries were between 95. 81% and 98. 73%, and the relative standard deviations (RSD) were between 3. 20 and 4. 77(n=6). This method is simple,fast,accurate and reproducible, and the result is reliable. The method is applicable for the determination of 12 phenolic acids and isoflavones in Iris pseudozcorus.