Rapid semiquantitative estimation of benzene hexachloride by micro‐t.l.c.

Abstract

The relationship between the spot concentration and the resolution of different components in commercial hexachlorocyclohexane with micro‐ and macro‐thin layer chromatography has been studied. Of the different spot concentrations investigated, only 0.025 μg on micro‐t.l.c. and 1.0 μg on macro‐t.l.c. gave two clear spots which can be made use of for the rapid semiquantitative estimation of benzene hexachloride in insecticide formulations. The application of this technique has been further investigated on different commercial benzene hexachloride dust formulations and the error ranged between 2 and 5%.