Rapid, selective and stable HaloTag-<i>Lb</i>ADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

Döbber, Johannes; Pohl, Martina; Ley, Steven V.; Musio, Biagia

doi:10.1039/c7re00173h

Cited by 36 publications

(25 citation statements)

References 25 publications

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“…This set-up shows the potential of NADP(H) recycling for continuous operated systems within biocatalytic processes. Recently, also Döbber et al used the HaloTag-immobilization strategy for the continuous biocatalytic production of chiral alcohols and epoxides, 87 and for a continuous enzymatic cascade towards a vicinal chiral diol. 88 A recent publication correspondingly dealing with the cofactor regeneration in continuously operated biocatalytic reactions was published by Hartley et al in late 2019.…”

Section: Selected Examples Of Continuously Operated Meso-scale Reactomentioning

confidence: 99%

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

2020

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Section: Selected Examples Of Continuously Operated Meso-scale Reactomentioning

confidence: 99%

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

2020

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“…Furthermore, enzyme immobilization might increase enzyme stability,– for example, against higher temperatures or pH changes, and, in selected cases, can even enable the use of enzymes in organic solvents or neat substrate systems as well . However, immobilization might also negatively affect enzyme activity, or can furthermore lead to mass transport limitations . Additionally, immobilization is a cost‐driving factor for many applications in biocatalysis .…”

Section: Factors To Consider When Designing and Choosing Systems For mentioning

confidence: 99%

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

2019

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Multi‐step biocatalytic reactions have gained increasing importance in recent years because the combination of different enzymes enables the synthesis of a broad variety of industrially relevant products. However, the more enzymes combined, the more crucial it is to avoid cross‐reactivity in these cascade reactions and thus achieve high product yields and high purities. The selective control of enzyme activity, i.e., remote on‐/off‐switching of enzymes, might be a suitable tool to avoid the formation of unwanted by‐products in multi‐enzyme reactions. This review compiles a range of methods that are known to modulate enzyme activity in a stimulus‐responsive manner. It focuses predominantly on in vitro systems and is subdivided into reversible and irreversible enzyme activity control. Furthermore, a discussion section provides indications as to which factors should be considered when designing and choosing activity control systems for biocatalysis. Finally, an outlook is given regarding the future prospects of the field.

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“…By immobilizing biocatalysts, the stability towards organic solvents often can be improved significantly . In this context, the superabsorber‐based encapsulation of enzymes or whole cells in a packed bed reactor, has recently been investigated among many other systems based on, for example, carrier‐immobilization or immobilization in alginate beads , …”

Section: Introductionmentioning

confidence: 99%

Heterogeneous Catalysts “on the Move”: Flow Chemistry with Fluid Immobilised (Bio)Catalysts

Adebar

Gröger

2020

Eur J Org Chem

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As both, continuous synthesis and (bio-)catalysis gained increasing interest in research as well as for industrial applications, ways for merging these fields enables novel opportunities for modern sustainable process development. In this contribution, an alternative approach for the application of immobilized enzymes in continuous flow processes is presented utilizing heterogeneous biocatalysts as a mobile phase. Based on superabsorber-entrapped enzymes and whole cells as a "fluid heterogeneous phase", a segmented hydrogel/organic

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Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

Abstract: A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described.

Cited by 36 publications

References 25 publications

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

The rise of continuous flow biocatalysis – fundamentals, very recent developments and future perspectives

Stimulus‐Responsive Regulation of Enzyme Activity for One‐Step and Multi‐Step Syntheses

Heterogeneous Catalysts “on the Move”: Flow Chemistry with Fluid Immobilised (Bio)Catalysts

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