Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants

Wang, Na; Pei, Haiwei; Xiang, Wenzhou; Li, Tao; Lin, Songyi; Wu, Jiayi; Chen, Zishuo; Wu, Houbo; Li, Chuanmao; Wu, Hao

doi:10.3390/foods12142652

Cited by 9 publications

(8 citation statements)

References 79 publications

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“…Wang et al . (2023) reported that FRAP of A. platensis varied when it was extracted using different solvents, but ethanol/water fraction showed a higher FRAP activity than hexane and ethyl acetate fractions. Another study showed similar results that FRAP of S. platensis extracted using polar solvent assisted with microwave was higher than those extracted using non‐polar solvent (Esquivel‐Hernández et al ., 2017).…”

Section: Resultsmentioning

confidence: 99%

Colour characteristics, phenolic content and antioxidant activity of Spirulina platensis soaked in basil (Ocimum basilicum) leaves extract

Wijayanti,

Agustini,

Benjakul

2024

Int J of Food Sci Tech

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SummarySpirulina platensis has been known as a super food with a high nutrition and biological activity. The limited addition of S. platensis into the food is due to its unpleasant odour. S. platensis was soaked in basil (Ocimum basilicum) leaves extract (BLE) to decrease the unpleasant odour and its antioxidant activities after soaking were unknown. This study aimed to evaluate the effect of ratio S. platensis with BLE on colour and sensory characteristics and to determine the total phenolic content (TPC) and antioxidant capacity of extracted S. platensis without and with BLE using different solvents. Spirulina platensis was soaked in BLE at a ratio of S. platensis and BLE of 1:3, 1:4, and 1:5 (w/v) for 15 min and then dried at 40°C for 10 h. The resultant powder was analysed for colour and sensory characteristics. The selected S. platensis with the best sensory characteristic was further extracted in water and ethanol and was compared with the S. platensis control (unsoaked in BLE). The ratio of S. platensis and BLE had a significant effect on the colour characteristics (P < 0.05). Spirulina platensis soaked in BLE at a ratio 1:4 had higher L* (35.98), a* (−6.57) and b* values (5.95) than the control. The ratio of S. platensis and BLE at 1:4 had a higher overall sensory value (4.16) than the control (2.35). The water extract of S. platensis unsoaked and soaked in BLE showed a higher TPC (1.178 and 1.667 mg GAE g−1 extract, respectively), DPPH, ABTS and metal chelating, but lower in FRAP compared with the ethanol extract. Spirulina platensis soaked in BLE which were extracted in water and ethanol had higher ORAC (63.27 and 56.33 mmol Teq g−1 extract, respectively) than the control. Spirulina platensis soaked in BLE increased its sensory value while maintaining the antioxidant capacity. Therefore, it can potentially be added in more quantities to various foods as functional foods.

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Section: Resultsmentioning

confidence: 99%

Colour characteristics, phenolic content and antioxidant activity of Spirulina platensis soaked in basil (Ocimum basilicum) leaves extract

Wijayanti,

Agustini,

Benjakul

2024

Int J of Food Sci Tech

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“…Microalgae exhibit abundance in pigments, phenols, polysaccharides, proteins, essential fatty acids, vitamins, and other bioactive nutrients of high value, thereby presenting a potent repository of natural antioxidants. Notably, while the prevailing market for natural antioxidants predominantly relies on land plants, antioxidants derived from microalgae are presently not widely available [ 11 ]. Nevertheless, microalgae hold promise as a prospective source of natural antioxidant products due to their greater yield compared to terrestrial plants and the controllability of their culture conditions [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Light Influences the Growth, Pigment Synthesis, Photosynthesis Capacity, and Antioxidant Activities in Scenedesmus falcatus

Songserm,

Nishiyama,

Sanevas

2024

Scientifica

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Light plays a significant role in microalgae cultivation, significantly influencing critical parameters, including biomass production, pigment content, and the accumulation of metabolic compounds. This study was intricately designed to optimize light intensities, explicitly targeting enhancing growth, pigmentation, and antioxidative properties in the green microalga, Scenedesmus falcatus (KU.B1). Additionally, the study delved into the photosynthetic efficiency in light responses of S. falcatus. The cultivation of S. falcatus was conducted in TRIS-acetate-phosphate medium (TAP medium) under different light intensities of 100, 500, and 1000 μmol photons m−2·s−1 within a photoperiodic cycle of 12 h of light and 12 h of dark. Results indicated a gradual increase in the growth of S. falcatus under high light conditions at 1000 μmol photons m−2·s−1, reaching a maximum optical density of 1.33 ± 0.03 and a total chlorophyll content of 22.67 ± 0.2 μg/ml at 120 h. Conversely, a slower growth rate was observed under low light at 100 μmol photons m−2·s−1. However, noteworthy reductions in the maximum quantum yield (Fv/Fm) and actual quantum yield (Y(II)) were observed under 1000 μmol photons m−2·s−1, reflecting a decline in algal photosynthetic efficiency. Interestingly, these changes under 1000 μmol photons m−2·s−1 were concurrent with a significant accumulation of a high amount of beta-carotene (919.83 ± 26.33 mg/g sample), lutein (34.56 ± 0.19 mg/g sample), and canthaxanthin (24.00 ± 0.38 mg/g sample) within algal cells. Nevertheless, it was noted that antioxidant activities and levels of total phenolic compounds (TPCs) decreased under high light at 1000 μmol photons m−2·s−1, with IC50 of DPPH assay recorded at 218.00 ± 4.24 and TPC at 230.83 ± 86.75 mg of GAE/g. The findings suggested that the elevated light intensity at 1000 μmol photons m−2·s−1 enhanced the growth and facilitated the accumulation of valuable carotenoid pigment in S. falcatus, presenting potential applications in the functional food and carotenoid industry.

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“…Consequently, there has been considerable scienti c interest in identifying safe and natural exogenous antioxidant alternatives (Wang et al 2023).…”

Section: Introductionmentioning

confidence: 99%

“…For some time, terrestrial plants have been recognised as sources rich in antioxidants such as carotenoids, phenols and vitamins (Ferdous and Yusof 2021; Wang et al 2023). More recently, microalgae have emerged as potent and sustainable alternative sources with the capacity to produce diverse bioactive metabolites (Zhong and Shahidi 2015; Wang et al 2023). Several microalgal species such as Chlorella sp., Arthrospira sp.…”

Section: Introductionmentioning

confidence: 99%

Cytoprotective Effects of Microalgal Extracts on H 2 O 2 -Induced Oxidative Stress in Human Lung Adenocarcinoma A549 Cell Line

Herbert,

Touzet,

Campion

2024

Preprint

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The body's innate defences often fall short in combating oxidative stress in cells, highlighting the importance of integrating antioxidants from natural sources for preventative healthcare and nutrition. Microalgae-derived compounds like carotenoids and EPA-rich fatty acids play a critical role in protecting cells from oxidative stress. This study evaluated the antioxidant and cytoprotective properties of diethyl ether (Et2O) extracts prepared from the biomass of the microalgae Cyanophora paradoxa, Tetraselmis chuii and Phaeodactylum tricornutum, which were supplemented to human lung cancer cells (A549) experiencing oxidative stress. The extracts of T. chuii and C. paradoxa exhibited higher antioxidant content (123 and 150 µmol Trolox equivalent mg/Recovered Dried Material (RDM)) as compared to P. tricornutum when analysed using the FC assay. All extracts (150 µg mL− 1) demonstrated protective effects by attenuating H2O2-induced oxidative stress in A549 cells, as confirmed by the MTS (≤ 105% increase in cell growth) and DCF-DA assays (≤ 58% decrease in ROS production). RT-qPCR analysis revealed significant changes in the expression of apoptosis regulatory genes, with a decrease in the pro-apoptotic BAX gene (< 50%) and an increase in the anti-apoptotic Bcl-2 gene expression (> 200%), when cells were treated with extracts compared to cells treated with medium only after exposure to H2O2. These findings suggest that the microalgal extracts successfully mitigated the H2O2-induced oxidative stress in the A459 cells, likely attributable to their inherent antioxidative and apoptosis regulating properties. These attributes, which appear to restore homeostasis, present promising avenues for use in cosmetics and therapeutic contexts.

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Rapid Screening of Microalgae as Potential Sources of Natural Antioxidants

Cited by 9 publications

References 79 publications

Colour characteristics, phenolic content and antioxidant activity of Spirulina platensis soaked in basil (Ocimum basilicum) leaves extract

Colour characteristics, phenolic content and antioxidant activity of Spirulina platensis soaked in basil (Ocimum basilicum) leaves extract

Light Influences the Growth, Pigment Synthesis, Photosynthesis Capacity, and Antioxidant Activities in Scenedesmus falcatus

Cytoprotective Effects of Microalgal Extracts on H 2 O 2 -Induced Oxidative Stress in Human Lung Adenocarcinoma A549 Cell Line

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